[1]胡嘉莉,华 琳.miRNA-mRNA双重表达谱在前列腺癌分子调控机制中的作用[J].医学信息,2022,35(16):18-23.[doi:10.3969/j.issn.1006-1959.2022.16.004]
　HU Jia-li,HUA Lin.Role of miRNA-mRNA Dual Expression Profile in Molecular Regulation of Prostate Cancer[J].Journal of Medical Information,2022,35(16):18-23.[doi:10.3969/j.issn.1006-1959.2022.16.004]
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miRNA-mRNA双重表达谱在前列腺癌分子调控机制中的作用()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年16期
页码:
18-23
栏目:
生物信息学
出版日期:
2022-08-15

文章信息/Info

Title:
Role of miRNA-mRNA Dual Expression Profile in Molecular Regulation of Prostate Cancer
文章编号:
1006-1959（2022）16-0018-06
作者:
胡嘉莉; 华 琳
（首都医科大学生物医学工程学院，北京 100069）
Author(s):
HU Jia-li; HUA Lin
(School of Biomedical Engineering,Capital Medical University,Beijing 100069,China)
关键词:
前列腺癌; miRNA; mRNA; 分子调控机制
Keywords:
Prostate cancer; miRNA; mRNA; Molecular regulation mechanism
分类号:
R737.25
DOI:
10.3969/j.issn.1006-1959.2022.16.004
文献标志码:
A
摘要:
目的 对前列腺癌的mRNA和miRNA的双重表达谱进行生物信息学分析，探讨前列腺癌的分子调控机制并识别与前列腺癌相关的生物标记。方法 从GEO数据库中获取前列腺癌的mRNA和miRNA双重表达谱数据，首先采用差异表达分析提取与前列腺癌相关的上调和下调的miRNA和mRNA，然后通过Pearson相关分析确定最显著上调的和下调的miRNA与其靶向基因的反向调控关系，通过广义典型相关分析（GCCA）探讨miRNA和mRNA的分子调控机制并构建调控网络，识别与前列腺癌相关的生物标记。结果 共发现117个差异表达的miRNA和9054个差异表达的mRNA，其中有15个miRNA和35个mRNA表达下调，20个miRNA和36个mRNA表达上调；Pearson相关性分析显示，miR-137反向调控HOXC4、NPIPL3和WSB1，miR-367反向调控MEFA及TNFAIP6；GCCA分析显示，MED13L的第一主成分权系数在前列腺癌患者中最高，第一主成分权系数较高的TRRAP被证实与前列腺癌高度；miR-29b、miR-488*与前列腺癌高度关联且在miRNA-mRNA的分子调控网络中调控的基因数最多。结论 miR-29b和miR-29b-1*在前列腺癌相关的分子调控机制中有着重要的作用，可以作为潜在的阻止前列腺癌发生和发展的治疗靶向。
Abstract:
Objective To study the dual expression profile of mRNA and miRNA in prostate cancer by bioinformatics analyze, to explore the molecular regulation mechanism of prostate cancer and to identify biomarkers related to prostate cancer.Methods The mRNA and miRNA dual expression profile data of prostate cancer were obtained from GEO database. Firstly, the up-regulated and down-regulated miRNAs and mRNAs related to prostate cancer were extracted by differential expression analysis. Then, the reverse regulation relationship between the most significantly up-regulated and down-regulated miRNAs and their target genes was determined by Pearson correlation analysis. The molecular regulation mechanism of miRNA and mRNA was discussed by generalized canonical correlation analysis (GCCA) and the regulatory network was constructed to identify biomarkers related to prostate cancer.Results A total of 117 differentially expressed miRNAs and 9054 differentially expressed mRNAs were found, of which 15 miRNAs and 35 mRNAs were down-regulated and 20 miRNAs and 36 mRNAs were up-regulated. Pearson correlation analysis showed that miR-137 reversely regulated HOXC4, NPIPL3 and WSB1, and miR-367 reversely regulated MEFA and TNFAIP6; GCCA analysis showed that the first principal component weight coefficient of MED13L was the highest in prostate cancer patients, and TRRAP with higher first principal component weight coefficient was confirmed to be highly associated with prostate cancer. MiR-29b and miR-488* were highly associated with prostate cancer and regulated the most genes in the molecular regulatory network of miRNA-mRNA.Conclusion miR-29 b and miR-29 b-1* play an important role in the molecular regulation mechanism of prostate cancer, and can be used as potential therapeutic targets to prevent the occurrence and development of prostate cancer.

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更新日期/Last Update: 1900-01-01