[1]陈湘姣,杨 豪,梁 辉.右美托咪定对大鼠蛛网膜下腔出血后早期脑损伤的影响[J].医学信息,2022,35(23):61-68.[doi:10.3969/j.issn.1006-1959.2022.23.010]
　CHEN Xiang-jiao,YANG Hao,LIANG Hui.Effect of Dexmedetomidine on Early Brain Injury After Subarachnoid Hemorrhage in Rats[J].Journal of Medical Information,2022,35(23):61-68.[doi:10.3969/j.issn.1006-1959.2022.23.010]
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右美托咪定对大鼠蛛网膜下腔出血后早期脑损伤的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年23期
页码:
61-68
栏目:
论著
出版日期:
2022-12-01

文章信息/Info

Title:
Effect of Dexmedetomidine on Early Brain Injury After Subarachnoid Hemorrhage in Rats
文章编号:
1006-1959（2022）23-0061-08
作者:
陈湘姣; 杨 豪; 梁 辉
（湖南师范大学附属第一医院/湖南省人民医院神经内科，湖南 长沙 410000）
Author(s):
CHEN Xiang-jiao; YANG Hao; LIANG Hui
(Department of Neurology,the First Affiliated Hospital of Hunan Normal University/Hunan Provincial People’s Hospital,Changsha 410000,Hunan,China)
关键词:
蛛网膜下腔出血; 早期脑损伤; 右美托咪定; 自噬; 血脑屏障; 凋亡
Keywords:
Subarachnoid hemorrhage; Early brain injury; Dexmetomidine; Autophagy; Blood-brain barrier; Apoptosis
分类号:
R743.35
DOI:
10.3969/j.issn.1006-1959.2022.23.010
文献标志码:
A
摘要:
目的 探讨右美托咪定（DEX）干预对蛛网膜下腔出血（SAH）后自噬活性的影响以及其在SAH后早期脑损伤中的作用。方法 将48只SD大鼠随机分为4组：Sham组、SAH组、SAH+DEX组、SAH+3-MA组，每组12只，各组再分为两个亚组，每组6只。比较各组神经功能、脑含水量、伊文思蓝含量、细胞凋亡、Beclin-1和LC3-Ⅱ蛋白表达水平及自噬体数目和形态。结果 ①SAH组、SAH+DEX组、SAH+3-MA组造模后24、72 h神经功能评分均低于Sham组，SAH+DEX组、SAH+3-MA组造模后24 h神经功能评分高于SAH组（P＜0.05），但SAH+DEX组与SAH+3-MA组造模后24、72 h神经功能评分比较，差异无统计学意义（P＞0.05）；②SAH组、SAH+DEX组、SAH+3-MA组大鼠造模后24、72 h脑含水量、脑组织EB含量均高于Sham组，SAH组造模后24、72 h脑组织含水量高于SAH+DEX组和SAH+3-MA组（P＜0.05），但SAH+DEX组与SAH+3-MA组造模后24、72 h脑含水量、脑组织EB含量比较，差异无统计学意义（P＞0.05）；③SAH组、SAH+DEX组、SAH+3-MA组造模后24 h脑细胞凋亡率高于Sham组，SAH组造模后24 h脑细胞凋亡率高于SAH+DEX组、SAH+3-MA组（P＜0.05）；各组造模后72 h脑细胞凋亡率比较，差异无统计学意义（P＞0.05）；④透射电子显微镜下可见到大小不等的自噬空泡及自噬小体，其中SAH+DEX组、SAH+3-MA组自噬体数目较SAH组减少，而SAH+DEX组与SAH+3-MA组大鼠脑组织中自噬体数量无明显差异；⑤SAH组、SAH+DEX组、SAH+3-MA组造模后24、72 h Beclin-1蛋白表达量高于Sham组（P＜0.05）；SAH组造模后24、72 h Beclin-1和LC3-Ⅱ蛋白表达量均高于SAH+DEX组及SAH+3-MA组（P＜0.05）；SAH组、SAH+3-MA组造模后24、72 h LC3-Ⅱ蛋白表达量高于Sham组（P＜0.05），但SAH+DEX组和Sham组LC3-Ⅱ蛋白表达量比较，差异无统计学意义（P＞0.05）。结论 DEX可通过抑制细胞自噬及细胞凋亡、保护血脑屏障在SAH后早期脑损伤中起神经保护作用。
Abstract:
Objective To investigate the effect of dexmedetomidine (DEX) intervention on autophagy activity after subarachnoid hemorrhage (SAH) and its role in early brain injury after SAH.Methods Forty-eight SD rats were randomly divided into 4 groups: Sham group, SAH group, SAH+DEX group, SAH+3-MA group, with 12 rats in each group, and each group was divided into two subgroups, with 6 rats in each subgroups. The neurological function, brain water content, Evans blue content, cell apoptosis, Beclin-1 and LC3-II protein expression, autophagosome number and morphology were compared among the groups.Results ①The neurological function scores of SAH group, SAH+DEX group and SAH+3-MA group were lower than those of Sham group at 24 and 72 h after modeling, and the neurological function scores of SAH+DEX group and SAH+3-MA group were higher than those of SAH group at 24 h after modeling (P<0.05), but there was no significant difference in neurological function score between SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling (P>0.05); ②The brain water content and EB content in SAH group, SAH+DEX group and SAH+3-MA group were higher than those in Sham group at 24 and 72 h after modeling, and the brain water content in SAH group was higher than that in SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling (P<0.05), but there was no significant difference in brain water content and EB content between SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling (P>0.05). The apoptosis rate of brain cells in SAH group, SAH + DEX group and SAH + 3-MA group was higher than that in Sham group (P<0.05); ③The apoptosis rate of brain cells in SAH group, SAH + DEX group and SAH + 3-MA group was higher than that in Sham group at 24 h after modeling, and the apoptosis rate of brain cells in SAH group was higher than that in SAH+DEX group and SAH+3-MA group at 24 h after modeling (P<0.05), but there was no significant difference in the apoptosis rate of brain cells at 72 h after modeling in each group (P>0.05); ④Autophagic vacuoles and autophagosomes with different sizes were observed under transmission electron microscope, the number of autophagosomes in SAH+DEX group and SAH+ 3-MA group was less than that in SAH group, while there was no significant difference between SAH+DEX group and SAH+3-MA group; ⑤The expression of Beclin-1 protein in SAH group, SAH+DEX group and SAH+3-MA group was higher than that in Sham group at 24 and 72 h after modeling (P<0.05); The expression of Beclin-1 and LC3-Ⅱ protein in SAH group was higher than that in SAH+DEX group and SAH+3-MA group at 24 and 72 h after modeling (P<0.05); The expression of LC3-Ⅱ protein in SAH group and SAH+3-MA group was higher than that in Sham group at 24 and 72 h after modeling (P<0.05), but there was no significant difference in the expression of LC3-Ⅱ protein between SAH+DEX group and Sham group (P>0.05).Conclusion DEX can play a neuroprotective role in the early stage after subarachnoid hemorrhage by inhibiting autophagy and apoptosis, and protecting the blood-brain barrier.

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更新日期/Last Update: 1900-01-01