[1]李 芳,蒋海涛,张 恺,等.双层型脱细胞真皮基质对大鼠伤口愈合的实验研究[J].医学信息,2023,36(02):49-53.[doi:10.3969/j.issn.1006-1959.2023.02.010]
 LI Fang,JIANG Hai-tao,ZHANG Kai,et al.Experimental Study of Double-layer Acellular Dermal Matrix on Wound Healing in Rats[J].Journal of Medical Information,2023,36(02):49-53.[doi:10.3969/j.issn.1006-1959.2023.02.010]
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双层型脱细胞真皮基质对大鼠伤口愈合的实验研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年02期
页码:
49-53
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
Experimental Study of Double-layer Acellular Dermal Matrix on Wound Healing in Rats
文章编号:
1006-1959(2023)02-0049-05
作者:
李 芳蒋海涛张 恺
(1.徐州医科大学口腔医学院,江苏 徐州 221000;2.徐州医科大学附属医院口腔科,江苏 徐州 221000)
Author(s):
LI FangJIANG Hai-taoZHANG Kaiet al.
(1.School of Stomatology,Xuzhou Medical University,Xuzhou 221000,Jiangsu,China;2.Department of Stomatology,Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,Jiangsu,China)
关键词:
双层型脱细胞真皮基质皮肤缺损炎症创面愈合胶原纤维毛细血管
Keywords:
Double-layer acellular dermal matrixSkin defectInflammationWound healingCollagen fibersCapillary
分类号:
R622.1
DOI:
10.3969/j.issn.1006-1959.2023.02.010
文献标志码:
A
摘要:
目的 观察不同型脱细胞真皮基质对大鼠皮肤缺损愈合的影响,比较不同修复材料在皮肤缺损中的应用效果。方法 选取90只SD大鼠(来源于南京紫源生物科技有限公司)于2021年10月-2022年1月在徐州医科大学动物实验中心建立大鼠背部全层皮肤缺损模型,随机分为空白组、对照组及实验组,每组30只。空白组采用凡士林油纱修复,对照组采用单层型脱细胞真皮基质修复,实验组采用双层型脱细胞真皮基质修复,分别于术后第3、6、9天处死实验动物,采用大体观察记录创面愈合情况,将移植区域切除、固定并包埋在石蜡中进行HE染色、Masson染色,镜下观察各组创面愈合面积、炎症细胞密度、血管密度及纤维生长状况,分析创面真皮层的愈合情况。结果 3组大鼠术后第3天背部皮肤创面愈合率比较,差异无统计学意义(P>0.05);实验组和对照组术后第6天创面愈合率高于空白组,差异有统计学意义(P<0.05);实验组术后第9天创面愈合率高于对照组,差异有统计学意义(P<0.05);HE染色显示,术后第3、6天实验组和对照组的炎细胞密度和毛细血管密度均高于空白组,差异有统计学意义(P<0.05);术后第6、9天实验组的炎细胞密度低于对照组,差异有统计学意义(P<0.05);Masson染色显示,3组大鼠术后第3、6、9天成纤维数量均呈现逐渐增多趋势,术后第9天实验组成纤维细胞多于对照组,差异有统计学意义(P<0.05)。结论 相较于凡士林油纱,脱细胞真皮基质可促进上皮增生,加速创面的愈合。此外,双层型脱细胞真皮基质促进创面愈合速率快于单层型脱细胞真皮基质,并在一定程度上可减轻瘢痕的形成,值得临床应用。
Abstract:
Objective To observe the effect of different types of acellular dermal matrix on skin defect healing in rats, and to compare the effects of two kinds of repair materials.Methods Ninety SD rats (from Nanjing Ziyuan Biotechnology Co, LTD.) were selected from October 2021 to January 2022 in the Animal Experimental Center of Xuzhou Medical University to establish a full-layer skin defect model of rats, and were randomly divided into blank group, control group and experimental group, with 30 rats in each group. The blank group was repaired with vaseline gauze, the control group was repaired with single-layer acellular dermal matrix, and the experimental group was repaired with double-layer acellular dermal matrix.The experimental animals were sacrificed at 3 days, 6 days and 9 days after operation respectively, the wound healing was observed and recorded in general. The transplanted area was cut, fixed and embedded in paraffin for HE staining and Masson staining, while the wound healing area, inflammatory cell density, vascular density and fiber growth were observed under microscope in each group to understand the healing of the wound dermis.Results On the 3rd day after operation, there was no significant difference in the wound healing rate of the back skin of rats in each group (P>0.05). On the 6th day after operation, the wound healing rate of the experimental group and the control group was higher than that of the blank group, the difference is statistically significant (P<0.05). On the 9th day after operation, the wound healing rate of the experimental group was higher than that of the control group (P<0.05). HE results showed that the density of inflammatory cells and capillaries in the experimental group and control group were higher than those in the blank group on the 3rd and 6th day after operation , the difference is statistically significant (P<0.05), meanwhile the density of inflammatory cells in the experimental group was lower than that in the control group on the 6th and 9th day after operation, the difference is statistically significant (P<0.05). The results of Masson staining showed that the number of fibroblasts in the three groups increased gradually at 3, 6 and 9 days after operation, and the number of experimental fibroblasts was more than that in the control group at 9 days after operation, the difference is statistically significant (P<0.05).Conclusion Compared with vaseline gauze phase, acellular dermal matrix can promote epithelial hyperplasia and accelerate wound healing. In addition, double-layer acellular dermal matrix can promote wound healing faster than single-layer acellular dermal matrix, and reduce scar formation to a certain extent, which is worthy of clinical promotion.

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更新日期/Last Update: 1900-01-01