[1]李茗薇,黄 茹,严青秀,等.小鼠卵巢组织冷冻保存和自体移植技术的研究[J].医学信息,2024,37(06):120-124.[doi:10.3969/j.issn.1006-1959.2024.06.020]
　LI Ming-wei,HUANG Ru,YAN Qing-xiu,et al.Study on the Cryopreservation and Autologous Transplantation Technique of Mouse Ovarian Tissues[J].Journal of Medical Information,2024,37(06):120-124.[doi:10.3969/j.issn.1006-1959.2024.06.020]
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小鼠卵巢组织冷冻保存和自体移植技术的研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年06期
页码:
120-124
栏目:
论著
出版日期:
2024-03-15

文章信息/Info

Title:
Study on the Cryopreservation and Autologous Transplantation Technique of Mouse Ovarian Tissues
文章编号:
1006-1959（2024）06-0120-05
作者:
李茗薇; 黄 茹; 严青秀; 等
（1.安徽理工大学第一附属医院/淮南市第一人民医院妇产科，安徽 淮南 232007；2.安徽理工大学医学院，安徽 淮南 232063）
Author(s):
LI Ming-wei; HUANG Ru; YAN Qing-xiu; et al.
(1.Department of Obstetrics and Gynecology,the First Affiliated Hospital of Anhui University of Science and Technology/Huainan First People ’s Hospital,Huainan 232007,Anhui,China;2.School of Medicine,Anhui University of Science and Technology,Huainan 232063,Anhui,China)
关键词:
卵巢; 冷冻保存; 移植; 生育力保护
Keywords:
Ovary; Cryopreservation; Transplantation; Protection of fertility
分类号:
R713.6
DOI:
10.3969/j.issn.1006-1959.2024.06.020
文献标志码:
A
摘要:
目的 验证卵巢冷冻保存移植技术的安全性和有效性，为保护女性生育能力提供临床基础。方法 选取健康性成熟的成年小鼠30只，分为研究A组、研究B组和对照组，每组10只。研究组小鼠麻醉后取出右侧卵巢，将其放入含20%HAS的M2培养基中缓冲，再转移至冷冻保护液中冻存。7 d后复苏，移植于小鼠左侧卵巢位置（左侧卵巢已取出）。对照组小鼠卵巢行原位移植手术。移植术后第5天起每日对3组小鼠进行阴道脱落细胞学检测。术后第10天，研究A组、对照组小鼠与同品系雄鼠合笼，并于合笼第25天取卵巢进行形态学观察。研究B组小鼠在移植第15天取卵巢进行组织学观察。结果 对照组小鼠有完整规律的动情周期。研究A组、研究B组分别有8、7只恢复动情周期。移植后小鼠卵巢体积部分发生变化，10%消失、40%萎缩、50%无变化。移植第15天，卵巢切片可见大量原始卵泡。移植第25天，卵巢切片中卵泡数减少，原始卵泡难寻，可见次级卵泡、成熟卵泡及黄体。移植卵巢切片中的卵泡密度均低于正常卵巢。合笼第22天，研究A组共有2只鼠怀孕并分别产下3只和1只小鼠，产仔数量少于对照组。结论 小鼠卵巢组织冷冻保存和自体原位移植技术可行。
Abstract:
Objective To verify the safety and efficacy of ovarian cryopreservation and transplantation technique, and lay a clinical foundation for the protection of female fertility.Methods Totally 30 healthy sexually mature adult mice were selected and divided into study group A, study group B and control group, with 10 mice in each group. The mice in study groups were anesthetized and then had the right ovaries removed, buffered in a M2 medium containing 20%HAS, and then transferred to cryoprotectant for freezing. The ovaries were resuscitated 7 days later, and the right ovaries were transplanted to the position of left ovaries (the left ovaries had been removed). From the 5th day after transplantation, three groups received exfoliative vaginal cytology on a daily basis. On the 10th days after surgery, the mice in study group A were caged with male mice of the same strain, and their ovaries were removed to observe morphology on the 25th day of co-caging. The mice in study group B had their ovaries removed to observe histology on the 15th day of transplantation.Results The mice in the control group had a complete and regular oestrous cycle. In study group A and study group B, 8 and 7 animals restored estrous cycles, respectively. After transplantation, some mice showed changes in ovarian volume, 10% of the ovaries disappeared, 40% atrophied and 50% didn’t change. On the 15th day of transplantation, a lot of primordial follicles were visible on ovarian sections. On the 25th day of transplantation, the number of follicles on ovarian sections fell, and it was hard to find primordial follicles, and only secondary follicles, mature follicles and corpus luteum were visible. The density of follicles on the sections of transplanted ovaries was lower than that in normal ovaries. On the 22nd day of co-caging, two mice in study group A were pregnant and delivered 3 mice and 1 mouse respectively, the number of litters was lower than that of the control group.Conclusion It is feasible to perform cryopreservation and autologous orthotopic transplantation on the ovarian tissues of mice.

参考文献/References:

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更新日期/Last Update: 1900-01-01