[1]其木格,李更生,郭中华,等.小儿复方鸡内金咀嚼片指纹图谱及治疗泄泻的药效学研究[J].医学信息,2026,39(01):32-39.[doi:10.3969/j.issn.1006-1959.2026.01.005]
　Qimuge,LI Gengsheng,GUO Zhonghua,et al.Fingerprint of Child Compound Endothelium Corneum Chewable Tabletand its Pharmacodynamics in Treating Diarrhea[J].Journal of Medical Information,2026,39(01):32-39.[doi:10.3969/j.issn.1006-1959.2026.01.005]
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小儿复方鸡内金咀嚼片指纹图谱及治疗泄泻的药效学研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
39卷
期数:
2026年01期
页码:
32-39
栏目:
中医药信息学
出版日期:
2026-01-01

文章信息/Info

Title:
Fingerprint of Child Compound Endothelium Corneum Chewable Tabletand its Pharmacodynamics in Treating Diarrhea
文章编号:
1006-1959（2026）01-0032-08
作者:
其木格1; 李更生2; 郭中华1; 王晓冰1; 马 爽1
1.河南太龙药业股份有限公司，河南 郑州 450001；2.河南省中医药研究院，河南 郑州 450003
Author(s):
Qimuge1;  LI Gengsheng2;  GUO Zhonghua1;  WANG Xiaobing1;  MA Shuang1
1.Henan Taloph Pharmaceutical Co., Ltd., Zhengzhou 450001, Henan, China;2.Henan Institute of Traditional Chinese Medicine, Zhengzhou 450003, Henan, China
关键词:
小儿复方鸡内金咀嚼片; 高效液相色谱法; 指纹图谱; 药效学研究; 泄泻
Keywords:
Child compound endothelium corneum chewable tablet;  High performance liquid chromatography;  Fingerprint;  Pharmacodynamic study;  Diarrhea
分类号:
R285.5；R256.34
DOI:
10.3969/j.issn.1006-1959.2026.01.005
文献标志码:
A
摘要:
目的 建立小儿复方鸡内金咀嚼片（CCEC）的HPLC指纹图谱检测方法，并研究其对大黄致小鼠脾虚腹泻模型、腹泻主导型肠易激综合征（IBS-D）大鼠模型的影响。方法 采用高效液相色谱法建立CCEC的指纹图谱，对指纹图谱色谱峰进行药味归属并进行峰定位研究；采用国家药典委员会颁布的《中药色谱指纹图谱相似度评价软件系统2012年版》，以15批HPLC图谱色谱峰进行自动匹配，形成共有模式图，标定共有峰，并建立对照图谱R，同时与对照图谱R计算相似度。采用大黄致小鼠脾虚腹泻模型考察其对脾虚腹泻小鼠D-木糖吸收、胃排空及肠推进的影响；采用IBS-D大鼠模型考察其对IBS-D大鼠稀便级数、肠推进的影响。结果 建立了CCEC的指纹图谱，并对其中的色谱峰进行了药味归属，通过峰定位研究共指认出其中6种化学成分，同时建立了15批指纹图谱的共有模式图及对照图谱R，共标定17个共有峰，15批样品与对照图谱R的相似度均＞0.95。在CCEC对脾虚腹泻小鼠模型影响的研究中，与模型组相比，补脾消积口服液组与CCEC各剂量组排便数显著减少（P＜0.01）；与模型组相比，补脾消积口服液组、CCEC高、中剂量组D-木糖含量增加（P＜0.05），胃排空率降低（P＜0.05），肠推进率降低（P＜0.05）。在CCEC对IBS-D大鼠模型影响的研究中，与模型组相比，曲美布汀组和CCEC高剂量组稀便级数以及肠推进率显著降低（P＜0.01）。结论 本研究所建立的HPLC指纹图谱检测方法操作便捷，结果准确，重复性好，可全面有效地评价CCEC的质量。CCEC可以提高脾虚腹泻小鼠D-木糖吸收，抑制脾虚腹泻小鼠胃排空及肠推进；可以抑制IBS-D大鼠的稀便级数、抑制肠推进；CCEC的药效学研究表明其具有治疗泄泻及IBS-D的作用，与其临床应用相符合，为其治疗泄泻的临床应用提供了一定的药效学依据。
Abstract:
Objective To establish HPLC fingerprint detection method of child compound endothelium corneum (CCEC) chewable tablet, and to study its effect on Dahuang induced spleen deficiency diarrhea model in mice and irritable bowel syndrome with diarrhea (IBS-D) model in rats. Methods The fingerprint of CCEC was established by HPLC, and the peak of the fingerprint chromatogram was classified and the peak location was studied. Using the Software System for Similarity Evaluation of TCM Chromatographic Fingerprint issued by the National Pharmacopoeia Committee, 15 batches of HPLC chromatographic peaks were automatically matched to form a common pattern map, the common peaks were calibrated, and the control map R was established, and the similarity was calculated with the control map R. A rat model of spleen deficiency diarrhea induced by Dahuang was used to investigate the effects of CCEC on D-xylose absorption, gastric emptying and intestinal propulsion. A rat model of IBS-D was used to investigate the effects of CCEC on the number of loose stools and intestinal propulsion. Results The fingerprint of CCEC was established, and the chromatographic peaks in it were attributed. A total of 6 chemical components were identified through the peak localization study. At the same time, the common pattern map and the control map R of 15 batches of fingerprints were established, and a total of 17 common peaks were labeled. The similarity between the 15 batches of samples and the control spectrum R was greater than 0.95. In the study of the effect of CCEC on spleen deficiency diarrhea rat model, compared with the model group, the number of defecation in Bupi Xiaoji oral liquid group and CCEC dose group was significantly reduced (P<0.01). Compared with model group, the content of D-xylose in Bupi Xiaoji oral liquid group, CCEC high dose group and CCEC medium dose group was increased (P<0.05), the gastric emptying rate was decreased (P<0.05), and the intestinal propulsion rate was decreased (P<0.05). In the study of the effect of CCEC on IBS-D rat models, the number of loose stools and intestinal propulsion rate in the trimebutine group and the CCEC high dose group were significantly reduced compared with the model group (P<0.01). Conclusion The HPLC fingerprint detection method established in this study is convenient, accurate and reproducible, and can evaluate the quality of CCEC comprehensively and effectively. CCEC can improve D-xylose absorption and inhibit gastric emptying and intestinal propulsion in mice with spleen deficiency diarrhea. It can inhibit the number of loose stools and intestinal propulsion in IBS-D rats. The pharmacodynamic study of CCEC shows that it has the effect of treating diarrhea and IBS-D, which is consistent with its clinical application. This study provides a certain pharmacodynamic basis for its clinical application in the treatment of diarrhea.

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更新日期/Last Update: 1900-01-01