[1]赵有亮,付晓云.绣球防风提取物对LPS诱导RAW264.7细胞NO表达研究[J].医学信息,2026,39(01):45-48.[doi:10.3969/j.issn.1006-1959.2026.01.007]
　ZHAO Youliang,FU Xiaoyun.Study on the Effect of Leucas Ciliata Benth. Extract on NO Expression in LPS-induced RAW264.7 Cells[J].Journal of Medical Information,2026,39(01):45-48.[doi:10.3969/j.issn.1006-1959.2026.01.007]
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绣球防风提取物对LPS诱导RAW264.7细胞NO表达研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
39卷
期数:
2026年01期
页码:
45-48
栏目:
中医药信息学
出版日期:
2026-01-01

文章信息/Info

Title:
Study on the Effect of Leucas Ciliata Benth. Extract on NO Expression in LPS-induced RAW264.7 Cells
文章编号:
1006-1959（2026）01-0045-04
作者:
赵有亮; 付晓云
楚雄医药高等专科学校药学院，云南 楚雄 675000
Author(s):
ZHAO Youliang;  FU Xiaoyun
School of Pharmacy, Chuxiong Medical College, Chuxiong 675000, Yunnan, China
关键词:
绣球防风; RAW264.7细胞; 脂多糖; 一氧化氮
Keywords:
Leucas ciliata Benth.;  RAW264.7 cells;  Lipopolysaccharide;  Nitric oxide
分类号:
R285
DOI:
10.3969/j.issn.1006-1959.2026.01.007
文献标志码:
A
摘要:
目的 研究中药绣球防风提取物对脂多糖（LPS）诱导RAW264.7细胞一氧化氮（NO）表达抑制作用及其分子机制。方法 采用CCK-8法检测不同浓度绣球防风提取物对RAW264.7细胞活力的影响，筛选安全有效的药物浓度范围；采用Griess法检测细胞培养上清液中NO释放水平，反映绣球防风提取物对LPS诱导的RAW264.7细胞NO表达的调节作用。结果 200 μg/ml及以内的给药浓度下，绣球防风提取物对RAW264.7细胞活力无明显影响（P＞0.05）；25 μg/ml及以上浓度的绣球防风提取物可显著抑制RAW264.7炎症细胞NO的释放（P＜0.001），且呈现浓度梯度效应。结论 绣球防风提取物可有效抑制LPS诱导的RAW264.7细胞中NO的过量表达，且抑制效果与提取物浓度密切相关。
Abstract:
Objective To investigate the inhibitory effect of the traditional Chinese medicine Leucas ciliata Benth. extract on lipopolysaccharide (LPS)-induced nitric oxide (NO) expression in RAW264.7 cells and its molecular mechanism. Methods The impact of different concentrations of Leucas ciliata Benth. extract on RAW264.7 cell viability was assessed using the CCK-8 assay to determine the safe and effective concentration range. The Griess method was used to measure NO release levels in cell culture supernatants, reflecting the regulatory effect of the Leucas ciliata Benth. extract on LPS-induced NO expression in RAW264.7 cells. Results At concentrations up to 200 μg/ml, Leucas ciliata Benth. extract showed no significant effect on RAW264.7 cell viability (P>0.05). Concentrations of 25 μg/ml and higher significantly inhibited NO release in LPS-stimulated RAW264.7 cells (P<0.001), exhibiting a concentration-dependent effect. Conclusion Leucas ciliata Benth. extract effectively inhibits LPS-induced excessive NO expression in RAW264.7 cells, with the inhibitory effect closely correlated with the concentration of the extract.

参考文献/References:

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更新日期/Last Update: 1900-01-01