[1]刘 华,康美尼,王兴华,等.替格瑞洛对oxLDL诱导的人脐静脉内皮细胞ICAM-1表达的影响[J].医学信息,2018,31(22):81-84.[doi:10.3969/j.issn.1006-1959.2018.22.022]
　LIU Hua,KANG Mei-ni,WANG Xing-hua,et al.Effect of Ticagrelor on the Expression of ICAM-1 in Human umbilical Vein Endothelial Cells Induced by oxLDL[J].Journal of Medical Information,2018,31(22):81-84.[doi:10.3969/j.issn.1006-1959.2018.22.022]
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替格瑞洛对oxLDL诱导的人脐静脉内皮细胞ICAM-1表达的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年22期
页码:
81-84
栏目:
论著
出版日期:
2018-11-15

文章信息/Info

Title:
Effect of Ticagrelor on the Expression of ICAM-1 in Human umbilical Vein Endothelial Cells Induced by oxLDL
文章编号:
1006-1959（2018）22-0081-04
作者:
刘 华1; 2; 康美尼2; 王兴华2; 梁 雪2; 李广平2
（1.河北省沧州市中心医院心内科，河北 沧州 061000；2.天津医科大学第二医院心脏病学研究所，天津 300211）
Author(s):
LIU Hua1; 2; KANG Mei-ni2; WANG Xing-hua2; LIANG Xue2; LI Guang-ping2
(1.Department of Cardiology,Cangzhou Central Hospital,Cangzhou 061000,Hebei,China;2.Institute of Cardiology,Second Hospital,Tianjin Medical University,Tianjin 300211,China)
关键词:
替格瑞洛; 人脐静脉内皮细胞; 氧化型低密度脂蛋白; 细胞间黏附分子-1
Keywords:
Ticagrelor; Human umbilical vein endothelial cells; Oxidized low density lipoprotein;  Intercellular adhesion molecule-1
分类号:
R543
DOI:
10.3969/j.issn.1006-1959.2018.22.022
文献标志码:
A
摘要:
目的 探究不同浓度的替格瑞洛对氧化型低密度脂蛋白诱导的人脐静脉内皮细胞在蛋白及RNA水平表达细胞间黏附分子-1的影响。方法 离体培养人脐静脉内皮细胞，平均接种于6孔培养板，待细胞生长至融合状态时取三孔加入ox-LDL（50 μg/ml），再分别加入不同浓度的替格瑞洛（0、20、40 μmol/L）。刺激干预24 h后采用Western Blot法测定ICAM-1的蛋白表达水平。重复实验，采用Real-time PCR法测定ICAM-1的RNA表达水平。结果 ①oxLDL能明显上调ICAM-1的表达；②Western Blot蛋白定量结果显示，oxLDL诱导组ICAM-1的蛋白表达明显高于对应的非诱导组（P＜0.05）；未予替格瑞洛干预组ICAM-1的蛋白表达高于替格瑞洛低浓度组（P＜0.05）；替格瑞洛低浓度组ICAM-1的蛋白表达高于替格瑞洛高浓度组（P＜0.05）；③Real-time PCR结果与Western Blot蛋白定量结果一致。结论 oxLDL能在RNA水平提高ICAM-1表达，替格瑞洛能在RNA水平抑制ICAM-1的表达，并与浓度正相关。
Abstract:
Objective To investigate the effects of different concentrations of ticagrelor on the expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells induced by oxidized low density lipoprotein at protein and RNA levels.Methods Human umbilical vein endothelial cells were cultured in vitro and seeded on a 6-well culture plate. When the cells were grown to the fusion state, ox-LDL (50 μg/ml) was added to the wells, and different concentrations of ticagrelor were added(0, 20, 40 μmol/L). The protein expression level of ICAM-1 was determined by Western Blot after 24 h of stimulation. The experiment was repeated and the RNA expression level of ICAM-1 was determined by Real-time PCR. Results ①oxLDL could up-regulate the expression of ICAM-1.②Western Blot protein quantitation results showed that the protein expression of ICAM-1 in oxLDL-induced group was significantly higher than that in the corresponding non-inducing group(P<0.05);The protein expression of ICAM-1 in the Ticagrelor-free group was higher than that in the tigrisin group (P<0.05). The protein expression of ICAM-1 in the low-concentration group was higher than that in the Ticagrelor group(P<0.05); ③Real-time PCR results were consistent with Western Blot protein quantitation results. Conclusion oxLDL can increase the expression of ICAM-1 at the RNA level, and ticagrelor can inhibit the expression of ICAM-1 at the RNA level, and it is positively correlated with the concentration.

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更新日期/Last Update: 2018-12-03