[1]马晓云,田琳琳,李代清,等.生物钟节律紊乱影响糖尿病小鼠糖代谢及胰岛素分泌的实验研究[J].医学信息,2019,32(23):61-63,69.[doi:10.3969/j.issn.1006-1959.2019.23.017]
　MA Xiao-yun,TIAN Lin-lin,LI Dai-qing,et al.Experimental Study on the Effects of Biological Clock Rhythm Disorder on Glucose Metabolism and Insulin Secretion in Diabetic Mellitus Mice[J].Medical Information,2019,32(23):61-63,69.[doi:10.3969/j.issn.1006-1959.2019.23.017]
点击复制

生物钟节律紊乱影响糖尿病小鼠糖代谢及胰岛素分泌的实验研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年23期
页码:
61-63,69
栏目:
论著
出版日期:
2019-12-01

文章信息/Info

Title:
Experimental Study on the Effects of Biological Clock Rhythm Disorder on Glucose Metabolism and Insulin Secretion in Diabetic Mellitus Mice
文章编号:
1006-1959（2019）23-0061-04
作者:
马晓云; 田琳琳; 李代清; 汤云昭
（天津市代谢性疾病重点实验室/国家卫健委激素与发育重点实验室/天津医科大学朱宪彝纪念医院/天津医科大学，天津 300134）
Author(s):
MA Xiao-yun; TIAN Lin-lin; LI Dai-qing; TANG Yun-zhao
(Tianjin Key Laboratory of Metabolic Diseases/National Health and Safety Commission Key Laboratory of Hormone and Development/Tianjin Medical University Zhu Xianyi Memorial Hospital/Tianjin Medical University,Tianjin 300134,China)
关键词:
生物钟节律; 糖尿病; 血糖; 胰岛素分泌
Keywords:
Biological clock rhythm; Diabetes mellitus; Blood sugar; Insulin secretion
分类号:
R587.1
DOI:
10.3969/j.issn.1006-1959.2019.23.017
文献标志码:
A
摘要:
目的 探究生物钟节律紊乱对糖尿病肥胖小鼠（ob/ob）糖代谢及胰岛素分泌的影响。方法 选取同体重的6~8周龄C57BL/6及ob/ob小鼠各12只，随机分为对照组（C57组）、糖尿病组（OB组）、对照组+生物钟节律干扰组（C57+CSD组）、糖尿病组+生物钟节律干扰组（OB+CSD组），每组6只。C57+CSD组和OB+CSD组小鼠分别置于高台水环境下干扰其生物钟节律。C57组和OB组小鼠分别置于标准饲养笼中，其生物钟节律不受干扰，动物体重每天监测1次。4周后行小鼠体内腹腔注射葡萄糖耐量实验（IPGTT，2 g/kg），分离实验小鼠胰岛行体外葡萄糖刺激胰岛素分泌实验（GSIS）。结果 C57、C57+CSD和OB组体重分别增长22.92%、0.42%和26.16%，而OB+CSD组下降0.17%。空腹血糖测定显示：OB组与OB+CSD组血糖值水平高于C57组和C57+CSD组（P＜0.05）；IPGTT实验显示：OB组小鼠血糖峰值明显后移，且在15、30、60、90 min的血糖均高于C57组（P＜0.05）；C57+CSD组小鼠各时间点血糖均高于C57组（P＜0.05），OB+CSD组小鼠于120 min的血糖高于OB组（P＜0.05）；体内、体外胰岛素测定显示：C57胰岛素分泌低于OB组（P＜0.05），C57+CSD组胰岛素分泌低于C57组（P＜0.05），OB+CSD组血清及体外胰岛胰岛素分泌低于OB组（P＜0.05）。结论 生物钟节律是维护糖代谢稳态的重要环节，其紊乱可能导致正常及糖尿病小鼠胰岛素分泌的减低。
Abstract:
Objective To investigate the effects of biological clock rhythm disorder on glucose metabolism and insulin secretion in diabetic mellitus obese mice (ob/ob). Methods 12 C57BL/6 and ob/ob mice, aged 6-8 weeks, were randomly divided into control group (C57 group), diabetic mellitus group (OB group), control group + biological clock rhythm interference group (C57+). CSD group), diabetes mellitus group + biological clock rhythm interference group (OB+CSD group), 6 in each group. The C57+CSD group and the OB+CSD group were each placed in a high-level water environment to interfere with their biological clock rhythm. C57 and OB mice were placed in standard cages, respectively, and their clock rhythms were undisturbed. Animal weight was monitored once a day. After 4 weeks, the mice were intraperitoneally injected with glucose tolerance test (IPGTT, 2g/kg), and the islet glucose-stimulated insulin secretion test (GSIS) was isolated from the mice. Results The body weight of C57, C57+CSD and OB groups increased by 22.92%, 0.42% and 26.16%, respectively, while the OB+CSD group decreased by 0.17%. Fasting blood glucose measurement showed that the blood glucose level of OB group and OB+CSD group was higher than that of C57 group or C57+CSD group (P＜0.05). The IPGTT experiment showed that the blood glucose peak of OB group was significantly shifted back,the blood glucose at 15, 30, 60, 90 min was higher than that in C57 group (P＜0.05). The blood glucose in C57+CSD group was higher than that in C57 group (P＜0.05). OB+CSD mice The blood glucose at 120 min was higher than that in OB group (P＜0.05). In vivo and in vitro insulin assay showed that C57 insulin secretion was lower than that of OB group (P＜0.05), and insulin secretion in C57+CSD group was lower than that of C57 group (P＜0.05). The serum and in vitro insulin secretion of OB+CSD group was lower than that of OB group (P＜0.05). Conclusion The biological clock rhythm is an important link to maintain the homeostasis of glucose metabolism, and its disorder may lead to a decrease in insulin secretion in normal and diabetic mellitus mice.

参考文献/References:

[1]Aldawi N,Darwiche G,Abusnana S,et al.Initial increase in glucose variability during Ramadan fasting in non-insulin-treated patients with diabetes type 2 using continuous glucose monitoring[J].Libyan J Med,2019,14(1):1535747.[2]Gonzalez L,Garrie K,Turner M.Type 2 diabetes-An autoinflammatory disease driven by metabolic stress[J].Biochim Biophys Acta Mol Basis Dis,2018,1864(11):3805-3823.[3]Wang LM,Gao P,Zhang M,et al.Prevalence and Ethnic Pattern of Diabetes and Prediabetes in China in 2013[J].Jama-Journal of the American Medical Association,2017,317(24):2515-2523.[4]Wu T,ZhuGe F,Sun L,et al.Enhanced effect of daytime restricted feeding on the circadian rhythm of streptozotocin-induced type 2 diabetic rats [J].Am J Physiol Endocrinol Metab,2012,302(9):E1027-1035.[5]Tang Y,Meng L,Li D,et al.Interaction of sleep quality and sleep duration on glycemic control in patients with type 2 diabetes mellitus[J].Chin Med J(Engl),2014,127(20):3543-3547.[6]Leproult R,Holmback U,Van Canter E.Circadian Misalignment Augments Markers of Insulin Resistance and Inflammation,Independently of Sleep Loss[J].Diabetes,2014,63(6):1860-1869.[7]Asher G,Sassone-Corsi P.Time for Food:The Intimate Interplay between Nutrition,Metabolism, and the Circadian Clock [J].Cell,2015,161(1):84-92.[8]Li M,Li X,Lu Y.Obstructive Sleep Apnea Syndrome and Metabolic Diseases[J].Endocrinology,2018,159(7):2670-2675.[9]Hein M,Lanquart J,Loas G,et al.Prevalence and Risk Factors of Type 2 Diabetes in Major Depression:A Study on 703 Individuals Referred for Sleep Examinations[J].Psychosomatics,2018,59(2):144-157.[10]Paschos GK,FitzGerald GA.Circadian clocks and vascular function [J].Circ Res,2010,106(5):833-841.[11]Hida M,Anno T,Kawasaki F,et al.A rare case of large pyosalpinx in an elderly patient with well-controlled type 2 diabetes mellitus:a case report[J].J Med Case Rep,2018,12(1):286.[12]Bonnefond A,Froguel P.Disentangling the Role of Melatonin and its Receptor MTNR1B in Type 2 Diabetes:Still a Long Way to Go[J].Curr Diab Rep,2017,17(12):122.[13]Kent B,McNicholas W,Ryan S.Insulin resistance,glucose intolerance and diabetes mellitus in obstructive sleep apnoea[J].J Thorac Dis,2015,7(8):1343-1357.[14]Forrestel A,Miedlich S,Yurcheshen M,et al.Chronomedicine and type 2 diabetes:shining some light on melatonin[J].Diabetologia,2017,60(5):808-822.[15]Roh J,Lee J,Park S,et al.CRISPR-Cas9-mediated generation of obese and diabetic mouse models[J].Exp Nim,2018,67(2):229-237.[16]Barf RP,Van Dijk G,Scheurink AJW,et al.Metabolic consequences of chronic sleep restriction in rats:Changes in body weight regulation and energy expenditure[J].Physiology&Behavior,2012,107(3):322-328.[17]Jung CM,Melanson EL,Frydendall EJ,et al.Energy expenditure during sleep,sleep deprivation and sleep following sleep deprivation in adult humans[J].Journal of Physiology-London,2011,589(1):235-244.[18]Sgoifo A,Buwalda B,Roos M,et al.Effects of sleep deprivation on cardiac autonomic and pituitary-adrenocortical stress reactivity in rats[J].Psychoneuroendocrinology,2006,31(2): 197-208.[19]Pardon MC,Marsden CA.The long-term impact of stress on brain function:From adaptation to mental diseases[J].Neuroscience and Biobehavioral Reviews,2008,32(6):1071-1072.[20]De Kloet ER,Joels M,Holsboer F.Stress and the brain:From adaptation to disease[J].Nature Reviews Neuroscience,2005,6(6):463-475.

相似文献/References:

[1]张西园.糖尿病与结直肠癌患者预后相关研究进展[J].医学信息,2018,31(01):55.[doi:10.3969/j.issn.1006-1959.2018.01.020]
　ZHANG Xi-yuan.Research Progress in the Relationship between Diabetes and the Prognosis of Colorectal Cancer[J].Medical Information,2018,31(23):55.[doi:10.3969/j.issn.1006-1959.2018.01.020]
[2]耿金萍.综合护理对甲状腺良性肿瘤并糖尿病患者手术效果的影响分析[J].医学信息,2018,31(01):163.[doi:10.3969/j.issn.1006-1959.2018.01.064]
　GENG Jin-ping.Analysis of the Effect of Comprehensive Nursing on the Surgical Effect of Thyroid Benign Tumor and Diabetic Patients[J].Medical Information,2018,31(23):163.[doi:10.3969/j.issn.1006-1959.2018.01.064]
[3]李雯婷,谈世华,余江明,等.踝肱指数检测在住院糖尿病患者中的重复性研究[J].医学信息,2018,31(03):159.[doi:10.3969/j.issn.1006-1959.2018.03.058]
　LI Wen-ting,TAN Shi-hua,YU Jiang-ming,et al.Study on Reproducibility of Ankle-brachial Index in Inpatients with Diabetes Mellitus[J].Medical Information,2018,31(23):159.[doi:10.3969/j.issn.1006-1959.2018.03.058]
[4]刘 静.冠状动脉粥样硬化性心脏病合并糖尿病的血脂检验结果分析[J].医学信息,2018,31(04):154.[doi:10.3969/j.issn.1006-1959.2018.04.057]
　LIU Jing.Analysis of Blood Lipid Test Results in Coronary Atherosclerotic Heart Disease Complicated with Diabetes Mellitus[J].Medical Information,2018,31(23):154.[doi:10.3969/j.issn.1006-1959.2018.04.057]
[5]谢勇丽,苏晓清,张雅薇.糖尿病痛性周围神经病变患者情绪调查分析[J].医学信息,2018,31(05):14.[doi:10.3969/j.issn.1006-1959.2018.05.005]
　XIE Yong-li,SU Xiao-qing,ZHANG Ya-wei.Investigation and Analysis of Mood in Patients with Diabetic Peripheral Neuropathy[J].Medical Information,2018,31(23):14.[doi:10.3969/j.issn.1006-1959.2018.05.005]
[6]张雅薇,朱明慧.首发为动眼神经麻痹的糖尿病合并动眼神经麻痹误诊为痛性眼肌麻痹1例分析[J].医学信息,2018,31(05):23.[doi:10.3969/j.issn.1006-1959.2018.05.008]
　ZHANG Ya-wei,ZHU Ming-hui.An Analysis of a Case of Diabetes Mellitus with Oculomotor Nerve Palsy Misdiagnosed as Painful Ophthalmoplegia[J].Medical Information,2018,31(23):23.[doi:10.3969/j.issn.1006-1959.2018.05.008]
[7]张宇宁.黄酮类化合物治疗糖尿病及其并发症的研究[J].医学信息,2018,31(05):50.[doi:10.3969/j.issn.1006-1959.2018.05.018]
　ZHANG Yu-ning.Study on Flavonoids in the Treatment of Diabetes Mellitus and its Complications[J].Medical Information,2018,31(23):50.[doi:10.3969/j.issn.1006-1959.2018.05.018]
[8]柳 梅.头孢哌酮钠舒巴坦钠治疗糖尿病并发尿路感染的 临床效果观察[J].医学信息,2018,31(07):139.[doi:10.3969/j.issn.1006-1959.2018.07.047]
　LIU Mei.Clinical Efficacy of Cefoperazone Sodium and Sulbactam Sodium in Treatment of Diabetes Complicated with Urinary Tract Infection[J].Medical Information,2018,31(23):139.[doi:10.3969/j.issn.1006-1959.2018.07.047]
[9]吕桂英.影响糖尿病患者药物治疗依从性相关因素临床分析[J].医学信息,2018,31(08):109.[doi:10.3969/j.issn.1006-1959.2018.08.036]
　LV Gui-ying.Clinical Analysis of Factors Related to Compliance with Drug Therapy in Patients with Diabetes Mellitus[J].Medical Information,2018,31(23):109.[doi:10.3969/j.issn.1006-1959.2018.08.036]
[10]刘爱君.糖化血红蛋白测定在糖尿病诊断中的应用价值分析[J].医学信息,2018,31(08):161.[doi:10.3969/j.issn.1006-1959.2018.08.057]
　LIU Ai-jun.Analysis of the Value of Determination of Glycosylated Hemoglobin in the Diagnosis of Diabetes Mellitus[J].Medical Information,2018,31(23):161.[doi:10.3969/j.issn.1006-1959.2018.08.057]

更新日期/Last Update: 2019-12-01