[1]黄琪峰,郑琳琳,张 菁.甲状腺癌中miR-222关键靶基因预测及其信号通路分析[J].医学信息,2020,33(01):85-88.[doi:10.3969/j.issn.1006-1959.2020.01.026]
　HUANG Qi-feng,ZHENG Lin-lin,ZHANG Jing.Prediction of Key Target Genes of miR-222 in Thyroid Cancer and Analysis of Its Signal Pathway[J].Medical Information,2020,33(01):85-88.[doi:10.3969/j.issn.1006-1959.2020.01.026]
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甲状腺癌中miR-222关键靶基因预测及其信号通路分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年01期
页码:
85-88
栏目:
论著
出版日期:
2020-01-01

文章信息/Info

Title:
Prediction of Key Target Genes of miR-222 in Thyroid Cancer and Analysis of Its Signal Pathway
文章编号:
1006-1959（2020）01-0085-04
作者:
黄琪峰1; 郑琳琳2; 张 菁1
（浙江大学医学院附属邵逸夫医院药学部1，超声科2，浙江 杭州 310016）
Author(s):
HUANG Qi-feng1; ZHENG Lin-lin2; ZHANG Jing1
(Department of Pharmacy1,Department of Ultrasound2,Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,Zhejiang,China)
关键词:
甲状腺癌; miR-222; AGTR1; 生物信息学
Keywords:
Thyroid cancer; miR-222; AGTR1; Bioinformatics
分类号:
R736.1
DOI:
10.3969/j.issn.1006-1959.2020.01.026
文献标志码:
A
摘要:
目的 利用生物信息学技术预测人类miR-222的靶基因，并分析其可能参与甲状腺癌的生物学过程和信号通路。方法 通过TCGA数据库研究miR-222的表达与甲状腺癌的关系，然后TCGA中表达下调的基因和网站预测的miR-222靶基因进行取交集得到重叠基因，再进行生物信息学分析确定与甲状腺癌相关的关键miR-222靶基因和通路，最后通过GEPIA对靶基因进行表达验证以及miR-222和靶基因的相关性分析。结果 miR-222在甲状腺癌中表达上调，富集分析表明miR-222很有可能参与血管紧张素受体-配体结合的调节，其中一个可能的靶基因是AGTR1，其在甲状腺癌中表达下调，与miR-222的表达呈负相关。结论 上调的miR-222可能以AGTR1为靶标，从而调节甲状腺癌中血管紧张素受体-配体结合发挥作用。
Abstract:
Objective To predict the target genes of human miR-222 using bioinformatics technology, and analyze the biological processes and signal pathways that miR-222 may participate in. Methods TCGA database was used to study the relationship between miR-222 expression and thyroid cancer, then the genes with down-regulated expression in TCGA and miR-222 target genes predicted by the website were intersected to obtain overlapping genes, and bioinformatics analysis was performed to determine the correlation with thyroid cancer the key miR-222 target genes and pathways, and finally the target genes were verified by GEPIA and the correlation analysis between miR-222 and target genes. Results miR-222 expression was significantly up-regulated in thyroid cancer. Enrichment analysis showed that miR-222 is likely to be involved in the regulation of angiotensin receptor-ligand binding. One of the possible target genes is AGTR1, which is expressed in thyroid cancer.Down-regulation was significantly negatively correlated with miR-222 expression.Conclusion The up-regulated miR-222 is likely to target AGTR1, which may play a role in regulating angiotensin receptor-ligand binding in thyroid cancer.

参考文献/References:

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更新日期/Last Update: 2020-01-01