[1]张秀梅,赵俊军.TRIM32过表达对MCF-7乳腺癌细胞增殖、浸润和侵袭的影响[J].医学信息,2020,33(04):87-89.[doi:10.3969/j.issn.1006-1959.2020.04.026]
　ZHANG Xiu-mei,ZHAO Jun-jun.Effects of TRIM32 Overexpression on Proliferation,Infiltration and Invasion of MCF-7 Breast Cancer Cells[J].Medical Information,2020,33(04):87-89.[doi:10.3969/j.issn.1006-1959.2020.04.026]
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TRIM32过表达对MCF-7乳腺癌细胞增殖、浸润和侵袭的影响()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年04期
页码:
87-89
栏目:
论著
出版日期:
2020-02-15

文章信息/Info

Title:
Effects of TRIM32 Overexpression on Proliferation,Infiltration and Invasion of MCF-7 Breast Cancer Cells
文章编号:
1006-1959（2020）04-0087-03
作者:
张秀梅; 赵俊军
（大连医科大学附属大连市中心医院病理科，辽宁 大连 116033）
Author(s):
ZHANG Xiu-mei; ZHAO Jun-jun
(Department of Pathology,Dalian Central Hospital,Dalian Medical University,Dalian 116033,Liaoning,China)
关键词:
TRIM32; 过表达; 乳腺癌
Keywords:
TRIM32; Overexpression; Breast cancer
分类号:
R737.9
DOI:
10.3969/j.issn.1006-1959.2020.04.026
文献标志码:
A
摘要:
目的 探讨TRIM32过表达对MCF-7乳腺癌细胞增殖、浸润和侵袭的影响。方法 应用Western blot、CCK-8、Transwell和wound healing实验分析TRIM32过表达对MCF-7细胞增殖、浸润和侵袭影响。结果 Western blot实验显示，TRIM32在MCF-10A正常乳腺上皮细胞中低表达，而在MCF-7乳腺癌细胞中高表达（P＜0.05）；与空白对照组比较，MCF-7细胞中转染TRIM32质粒后TRIM32呈过表达（P＜0.05）；CCK-8实验显示，与空白对照组比较，TRIM32过表达后MCF-7细胞增殖率提高（P＜0.05）。Transwell实验显示，与空白对照组比较，过表达TRIM32后MCF-7乳腺癌细胞穿孔数目增加（P＜0.05）；Wound healing实验显示，与空白对照组比较，过表达TRIM32后MCF-7乳腺癌细胞12、24 h的迁移距离增加（P＜0.05）。结论 TRIM32在乳腺癌细胞中高表达，过表达TRIM32促进乳腺癌细胞增殖、浸润和侵袭能力，其可能成为乳腺癌治疗的新靶点。
Abstract:
Objective To investigate the effect of TRIM32 overexpression on the proliferation, infiltration and invasion of MCF-7 breast cancer cells. Methods Western blot, CCK-8, Transwell and wound healing experiments were used to analyze the effects of TRIM32 overexpression on MCF-7 cell proliferation, infiltration and invasion.Results Western blot experiments showed that TRIM32 was low-expressed in MCF-10A normal breast epithelial cells and high-expressed in MCF-7 breast cancer cells(P<0.05). Compared with the blank control group, MCF-7 cells were transfected with TRIM32 plasmid TRIM32 was over-expressed (P<0.05); CCK-8 experiments showed that compared with the blank control group, the proliferation rate of MCF-7 cells increased after TRIM32 over-expression (P<0.05). Transwell experiments showed that MCF-7 breast cancer cell perforations increased after overexpression of TRIM32 compared with the blank control group(P<0.05); Wound healing experiments showed that compared with the blank control group, MCF-7 breast cancer cells overexpressed TRIM32 migration distance at 12 and 24 h increased(P<0.05).Conclusion TRIM32 is highly expressed in breast cancer cells, and overexpression of TRIM32 promotes breast cancer cell proliferation, infiltration and invasion ability, which may become a new target for breast cancer treatment.

参考文献/References:

[1]Meroni G.Genomics and evolution of the TRIM gene family[J].Adv Exp Med Biol,2012(770):1-9. [2]Niehoff KM,Rajeevan N,Charpentier PA,et a1.Development of the tool to reduceappropriate medications(TRIM):A clinical decision support system to improve medication prescribing for older adults[J].Pharmacotherapy,2016,36(6):694-701. [3]Petrera F,Meroni G.TRIM proteins in development[J].Adv Exp Med Biol,2012(770):131-141. [4]Wang C,Xu J,Fu H,et al.TRIM32 promotes cell proliferation and invasion by activating β-catenin signaling in gastric cancer[J].J Cell Mol Med,2018,22(10):5020-5028. [5]Cui X,Lin Z,Chen Y,et al.Upregulated TRIM32 correlates with enhanced cell proliferation and poor prognosis in hepatocellular carcinoma[J].Mol Cell Biochem,2016,421(1-2):127-137. [6]Nicklas S,Otto A,Wu X,et al.TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration[J].PLoS One,2012,7(1):e30445. [7]Liu J,Zhang C,Wang XL,et al.E3 ubiquitin ligase TRIM32 negatively regulates tumor suppressor p53 to promote tumorigenesis[J].Cell Death Differ,2014,21(11):1792-1804. [8]Horn EJ,Albor A,Liu Y,et al.RING protein Trim32 associated with skin carcinogenesis has anti-apoptotic and E3-ubiquitin ligase properties[J].Carcinogenesis,2004,25(2):157-167. [9]Albor A,Kulesz-Martin M.Novel initiation genes in squamous cell carcinomagenesis:a role for substrate-specific ubiquitylation in the control of cell survival[J].Mol Carcinog,2007,46(8):585-590. [10]Ito M,Migita K,Matsumoto S,et al.Overexpression of E3 ubiquitin ligase tripartite motif 32 correlates with a poor prognosis in patients with gastric cancer[J].Oncol Lett,2017,13(5):3131-3138. [11]Zhao TT,Jin F,Li JG,et al.TRIM32 promotes proliferation and confers chemoresistance to breast cancer cells through activation of the NF-kB pathway[J].J Cancer,2018,9(8):1349-1356. [12]Kano S,Miyajima N,Fukuda S,et al.Tripartite motif protein 32 facilitates cell growth and migration via degradation of AbI-interactor-2[J].Cancer Res,2008,68(14):5572-5580. [13]Du YM,Zhang W,Du BH,et al.TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers[J].Onco Targets Ther,2018(11):7841-7852.

更新日期/Last Update: 2020-02-15