[1]张 蕊,耿 佳,王雪梅.长链非编码RNA在内皮细胞损伤模型中的表达调控研究[J].医学信息,2020,33(22):71-74,95.[doi:10.3969/j.issn.1006-1959.2020.22.022]
　ZHANG Rui,GENG Jia,WANG Xue-mei.Study on the Expression Regulation of Long Non-coding RNA in Endothelial Cell Injury Model[J].Medical Information,2020,33(22):71-74,95.[doi:10.3969/j.issn.1006-1959.2020.22.022]
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长链非编码RNA在内皮细胞损伤模型中的表达调控研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年22期
页码:
71-74,95
栏目:
论著
出版日期:
2020-11-15

文章信息/Info

Title:
Study on the Expression Regulation of Long Non-coding RNA in Endothelial Cell Injury Model
文章编号:
1006-1959（2020）22-0071-05
作者:
张 蕊; 耿 佳; 王雪梅
（1.西安医学院公共卫生学院卫生学教研室，陕西 西安 710021；2.新疆医科大学第一附属医院动物疾病模型研究重点实验室，新疆 乌鲁木齐 830054）
Author(s):
ZHANG Rui; GENG Jia; WANG Xue-mei
(1.Teaching and Research Office of Hygiene,Department of Public Health,Xi’an Medical University,Xi’an 710021,Shaanxi,China;2.the Key Laboratory of Animal Disease Model Research,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang,China)
关键词:
长链非编码RNA; 动脉粥样硬化; 人脐静脉内皮细胞; 氧化型低密度脂蛋白
Keywords:
Long non-coding RNA; Atherosclerosis; Human umbilical vein endothelial cells; Oxidized low-density lipoprotein
分类号:
R541.4
DOI:
10.3969/j.issn.1006-1959.2020.22.022
文献标志码:
A
摘要:
目的 研究不同应激条件下的内皮细胞（HUVECs）损伤模型中lncRNAs 的表达变化。方法 将HUVECs分为正常对照组、H2O2干预组、LPS诱导组及ox-LDL干预组，正常对照组细胞常规培养，H2O2干预组采用200 μmol/L 浓度的H2O2孵育12 h，LPS诱导组采用100 ng/ml LPS诱导6 h，ox-LDL干预组使用浓度为50 μg/ml ox-LDL的培养液诱导内皮细胞损伤48 h。建立模型后分别检测心梗相关lncRNA H19、MAIT、MALAT1、ANRIL在脐静脉内皮细胞中的mRNA表达；实时荧光定量法测定HUVECs中lncRNAs的表达，以及炎性因子VCAM-1、MCP-1和抗炎因子eNOS、IL-10、Arginine的mRNA表达；ELISA法检测培养上清液中促炎因子及抗炎因子的表达。结果 与对照组相比，ox-LDL干预组lncRNA H19、MAIT、ANRIL的表达均上调，差异有统计学意义（P＜0.05）；炎性因子MCP-1和VCAM-1的mRNA表达水平均上调（P＜0.05），eNOS的mRNA表达下调（P＜0.05），细胞上清液中的MCP-1、VCAM-1的表达增加（P＜0.05）。ox-LDL干预组的HUVECs细胞上清液中的eNOS活性低于空白对照组，差异有统计学意义（P＜0.05）。结论 lncRNA H19、MIAT、MALAT1、ANRIL参与调控了内皮细胞的脂质代谢。
Abstract:
Objective To study the expression changes of lncRNAs in endothelial cell (HUVECs) injury models under different stress conditions. Methods HUVECs were divided into normal control group, H2O2 intervention group, LPS induction group and ox-LDL intervention group. Normal control group cells were cultured routinely. The H2O2 intervention group was incubated with 200 μmol/L H2O2 for 12 h, and the LPS induction group was 100 ng/ml LPS was induced for 6 h, and the ox-LDL intervention group used a culture medium with a concentration of 50 μg/ml ox-LDL to induce endothelial cell damage for 48 h. After the model was established, the mRNA expression of myocardial infarction-related lncRNA H19, MAIT, MALAT1, and ANRIL in umbilical vein endothelial cells were detected; real-time fluorescence quantitative method was used to determine the expression of lncRNAs in HUVECs, as well as the inflammatory factors VCAM-1, MCP-1 And anti-inflammatory factors eNOS, IL-10, Arginine mRNA expression; ELISA method to detect the expression of pro-inflammatory factors and anti-inflammatory factors in the culture supernatant. Results Compared with the control group, the expressions of lncRNA H19, MAIT and ANRIL in the ox-LDL intervention group were all up-regulated,the difference was statistically significant (P<0.05); the mRNA expression levels of inflammatory factors MCP-1 and VCAM-1 were both up-regulated (P<0.05), eNOS mRNA expression was down-regulated(P<0.05), and the expression of MCP-1 and VCAM-1 in the cell supernatant increased(P<0.05). The eNOS activity in the HUVECs cell supernatant of the ox-LDL intervention group was lower than that of the blank control group,the difference was significant (P<0.05).Conclusion lncRNA H19, MIAT, MALAT1, ANRIL participate in the regulation of lipid metabolism of endothelial cells.

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更新日期/Last Update: 1900-01-01