[1]杨玉媛,刘有旺,李 腾,等.Th17细胞对动脉粥样硬化斑块易损性和斑块破裂的影响研究[J].医学信息,2018,31(18):53-56.[doi:10.3969/j.issn.1006-1959.2018.18.017]
　YANG Yu-yuan,LIU You-wang,LI Teng,et al.Effect of Th17 Cells on Atherosclerotic Plaque Vulnerability and Plaque Rupture[J].Journal of Medical Information,2018,31(18):53-56.[doi:10.3969/j.issn.1006-1959.2018.18.017]
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Th17细胞对动脉粥样硬化斑块易损性和斑块破裂的影响研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年18期
页码:
53-56
栏目:
论著
出版日期:
2018-09-15

文章信息/Info

Title:
Effect of Th17 Cells on Atherosclerotic Plaque Vulnerability and Plaque Rupture
文章编号:
1006-1959（2018）18-0053-04
作者:
杨玉媛; 刘有旺; 李 腾; 张晓东; 姚梦园; 宋泮泮; 李雅林
泰山医学院，山东 泰安 271000
Author(s):
YANG Yu-yuan; LIU You-wang; LI Teng; ZHANG Xiao-dong; YAO Meng-yuan; SONG Pan-pan; LI Ya-lin
Taishan Medical University,Tai'an 271000,Shandong,China
关键词:
关键词：Th17细胞; IL-17A; 动脉粥样硬化; 斑块易损性; 斑块破裂
Keywords:
Key words：Th17 cells; IL-17A; Atherosclerosis; Plaque vulnerability; Plaque rupture
分类号:
R392.32
DOI:
10.3969/j.issn.1006-1959.2018.18.017
文献标志码:
A
摘要:
目的 探讨Th17细胞及其主要效应性细胞因子IL-17A对动脉粥样硬化斑块易损性和斑块破裂的作用。方法 在Apoe-/-小鼠的颈动脉套置缩窄性套管并通过药物联合作用的方法建立小鼠动脉粥样硬化斑块破裂模型。将20只Apoe-/-小鼠进行颈动脉套管，套管后继续进行高脂饲料喂养，然后分为药物联合刺激的干预组和生理盐水对照组，每组10只，分别于套管后7周和15周处死小鼠进行检测。流式细胞术检测斑块破裂后Th17细胞的变化，ELISA检测细胞因子IL-17A的变化。同上方法制备斑块破裂模型，将30只模型小鼠分为IL-17A处理组和对照组。IL-17A处理组外源性给Apoe-/-小鼠腹腔注射IL-17A，对照组腹腔注射生理盐水，处理后2、5、7周检测对斑块大小、脂质沉积和胶原含量的影响，免疫组化染色检测巨噬细胞和平滑肌细胞在斑块局部的表达，评价IL-17A对斑块稳定性及斑块破裂的作用。结果 在动脉套管后7周，此时仅有斑块内出血时，干预组小鼠脾脏中Th17细胞的比例高于对照组（P＜0.05）；在动脉套管后15周，此时有明显斑块破裂时，两组小鼠脾脏中Th17细胞的比例统计学意义显著（P＜0.01）。对Th17细胞关键细胞因子IL-17A的检测发现，动脉套管后7周干预组小鼠与对照组相比，血清中IL-17A的水平无统计学意义，而15周干预组小鼠与对照组小鼠比较，血清中IL-17A的水平升高，统计学意义显著（P＜0.01）。外源性IL-17A处理5周可增加斑块易损性，7周更为明显。表现为处理7周斑块面积较对照组增大（P＜0.05），但纤维帽面积减小（P＜0.05），脂核面积增大（P＜0.05），帽/核比值降低（P＜0.01）。对斑块破裂的研究发现，干预组5周出现埋入式纤维帽斑块，7周出现埋入式纤维帽斑块例数增多。结论 Th17细胞及IL-17A具有促进动脉粥样斑块易损性和斑块破裂的作用。
Abstract:
Abstract：Objective To investigate the effects of Th17 cells and its main effector cytokine IL-17A on atherosclerotic plaque vulnerability and plaque rupture.Methods A model of atherosclerotic plaque rupture in mice was established by placing a constrictive cannula in the carotid artery of Apoe-/-mice and by a combination of drugs.20 Apoe-/-mice were subjected to carotid cannula,and the cannula was continued for high-fat diet feeding.Then,the drug-stimulated intervention group and the saline control group were used,and each group was 10,respectively,after the cannula.Mice were sacrificed at 7 and 15 weeks for testing.Flow cytometry was used to detect changes in Th17 cells after plaque rupture,and cytokine IL-17A was detected by ELISA.The plaque rupture model was prepared as above,and 30 model mice were divided into IL-17A treatment group and control group.The IL-17A treatment group exogenously injected IL-17A into the Apoe-/-mice,and the control group received intraperitoneal injection of normal saline.The effects of plaque size,lipid deposition and collagen content were detected at 2,5,and 7 weeks after treatment. Immunohistochemical staining was used to detect the expression of macrophages and smooth muscle cells in plaques,and the effect of IL-17A on plaque stability and plaque rupture was evaluated.Results At 7 weeks after the arterial cannula,when there was only intra-plaque hemorrhage,the proportion of Th17 cells in the spleen of the intervention group was higher than that of the control group (P<0.05);15 weeks after the arterial cannula,there were obvious spots at this time.When the block ruptured,the proportion of Th17 cells in the spleen of the two groups of mice was statistically significant(P<0.01).The detection of IL-17A,a key cytokine of Th17 cells, showed that there was no statistically significant difference in serum IL-17A between the intervention group and the control group at 7 weeks after arterial intubation,in the 15-week intervention group,the serum IL-17A level was increased compared with the control group,which was statistically significant(P<0.01).Exogenous IL-17A treatment for 5 weeks increased plaque vulnerability, which was more pronounced at 7 weeks.The area of plaques treated for 7 weeks was increased compared with the control group(P<0.05),but the fiber cap area decreased(P<0.05),the lipid nucleus area increased(P<0.05),and the cap/nuclear ratio decreased(P< 0.01).A study of plaque rupture found that there were embedded fibrous cap plaques in the intervention group for 5 weeks,and the number of embedded fibrous cap plaques increased in 7 weeks.Conclusion Th17 cells and IL-17A have the effect of promoting atherosclerotic plaque vulnerability and plaque rupture.

参考文献/References:


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更新日期/Last Update: 2018-09-15