[1]曹宝全,朱 洪.基于生物信息学方法分析KIF20A与肝癌预后的关系[J].医学信息,2021,34(15):47-49,53.[doi:10.3969/j.issn.1006-1959.2021.15.013]
 CAO Bao-quan,ZHU Hong.Analysis of the Relationship Between KIF20A and the Prognosis of Liver Cancer Based on Bioinformatics Methods[J].Medical Information,2021,34(15):47-49,53.[doi:10.3969/j.issn.1006-1959.2021.15.013]
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基于生物信息学方法分析KIF20A与肝癌预后的关系()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年15期
页码:
47-49,53
栏目:
论著
出版日期:
2021-08-01

文章信息/Info

Title:
Analysis of the Relationship Between KIF20A and the Prognosis of Liver Cancer Based on Bioinformatics Methods
文章编号:
1006-1959(2021)15-0047-04
作者:
曹宝全朱 洪
(昆明医科大学第二附属医院肝胆胰外科一病区,云南 昆明 650000)
Author(s):
CAO Bao-quanZHU Hong
(The First Ward of Hepatobiliary and Pancreatic Surgery,the Second Affiliated Hospital of Kunming Medical University, Kunming 650000,Yunnan,China)
关键词:
肝癌KIF20AmRNAs
Keywords:
Liver cancerKIF20AmRNAs
分类号:
R735.7
DOI:
10.3969/j.issn.1006-1959.2021.15.013
文献标志码:
A
摘要:
目的 利用生物信息学方法分析KIF20A与肝癌的关系。方法 基于GEO数据库中的肝癌数据集GSE121248使用edgeR来筛查差异的mRNAs,再对这些基因进行富集分析以探索其功能和潜在的作用通路;另基于String数据库分析后构建蛋白互作网络(PPI),经cytoscape可视化后得到关键基因,之后再在GEPIA数据库中验证。结果 共有531个差异表达的基因,其中上调的mRNAs有152个,下调的mRNAs有379个。DAVID数据库分析显示,531个差异基因的生物学功能主要富集在炎症反应、氧化过程和免疫反应中;细胞成分主要集中在细胞外区域,分子功能主要集中在氧化还原酶活性、蛋白质同源二聚活性、铁离子结合和血红蛋白结合;富集的主要途径是化学致癌性、代谢和抗生素生物合成。通过String数据库的分析和cytoscape软件的结合,只筛选出一个关键mRNA为KIF20A。生存曲线分析显示,KIF20A的表达与肝癌患者的预后相关,且低表达与高生存率呈正相关(P<0.05)。结论 KIF20A是该调控网络的核心基因,在肝癌的发生发展中起着重要作用,其高表达与患者生存率低有关,是判断肝癌预后的可靠生物标志物,可作为肝癌患者药物治疗的靶点。
Abstract:
Objective To analyze the relationship between KIF20A and liver cancer using bioinformatics methods.Methods Based on the liver cancer data set GSE121248 in the GEO database, edgeR was used to screen differential mRNAs, and then these genes were enriched and analyzed to explore their functions and potential pathways;In addition, a protein interaction network (PPI) was constructed based on the analysis of the String database, and the key genes were obtained after visualization by cytoscape, and then verified in the GEPIA database.Results There were a total of 531 differentially expressed genes, of which 152 were up-regulated mRNAs and 379 were down-regulated mRNAs.DAVID database analysis showed that the biological functions of 531 differential genes were mainly enriched in inflammatory response, oxidation process and immune response;Cell components were mainly concentrated in the extracellular area, and molecular functions were mainly concentrated in oxidoreductase activity, protein homodimerization activity, iron ion binding and hemoglobin binding;The main ways of enrichment were chemical carcinogenicity, metabolism and antibiotic biosynthesis.Through the combination of String database analysis and cytoscape software, only one key mRNA was selected as KIF20A.Survival curve analysis showed that the expression of KIF20A was correlated with the prognosis of liver cancer patients, and low expression was positively correlated with high survival rate (P<0.05).Conclusion KIF20A is the core gene of this regulatory network and plays an important role in the occurrence and development of liver cancer.Its high expression is related to the low survival rate of patients, is a reliable biomarker for judging the prognosis of liver cancer, and can be used as a target of drug treatment for liver cancer patients.

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更新日期/Last Update: 1900-01-01