[1]李佳楠,陈春悠.STAT3、NCK1和VEGF在甲状腺乳头状癌中的表达及临床意义[J].医学信息,2022,35(05):94-97.[doi:10.3969/j.issn.1006-1959.2022.05.023]
　LI Jia-nan,CHEN Chun-you.Expression of STAT3, NCK1 and VEGF in Papillary Thyroid Carcinoma and its Clinical Significance[J].Medical Information,2022,35(05):94-97.[doi:10.3969/j.issn.1006-1959.2022.05.023]
点击复制

STAT3、NCK1和VEGF在甲状腺乳头状癌中的表达及临床意义()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年05期
页码:
94-97
栏目:
论著
出版日期:
2022-03-01

文章信息/Info

Title:
Expression of STAT3, NCK1 and VEGF in Papillary Thyroid Carcinoma and its Clinical Significance
文章编号:
1006-1959（2022）05-0094-04
作者:
李佳楠; 陈春悠
（1.华北理工大学口腔医学院，河北 唐山 063000；2.唐山市工人医院头颈外科，河北 唐山 063000）
Author(s):
LI Jia-nan; CHEN Chun-you
(1.School of Stomatology,North China University of Science and Technology,Tangshan 063000,Hebei,China;2.Head and Neck Surgery,Tangshan Workers’ Hospital,Tangshan 063000,Hebei,China)
关键词:
甲状腺乳头状癌; STAT3; NCK1; VEGF; 肿瘤血管生成; 颈部淋巴结转移
Keywords:
Papillary thyroid carcinoma; STAT3; NCK1; VEGF; Tumor angiogenesis; Cervical lymph node metastasis
分类号:
R736.1
DOI:
10.3969/j.issn.1006-1959.2022.05.023
文献标志码:
A
摘要:
目的 研究STAT3、NCK1、VEGF在甲状腺乳头状癌中的表达情况及临床意义。方法 收集2019年9月-2020年12月在唐山市工人医院头颈外科经病理诊断明确的甲状腺乳头状癌组织63例，甲状腺腺瘤组织32例，正常甲状腺组织20例。使用免疫组织化学法检测STAT3、NCK1、VEGF的表达水平，分析不同甲状腺组织中STAT3、NCK1、VEGF蛋白表达情况，以及甲状腺乳头状癌中STAT3、NCK1、VEGF蛋白与临床病理的关系。结果 甲状腺乳头状癌中STAT3、NCK1、VEGF阳性表达率高于甲状腺腺瘤和正常甲状腺组织，差异有统计学意义（P＜0.05）；而甲状腺腺瘤和正常甲状腺组织中STAT3、NCK1、VEGF阳性表达率比较，差异无统计学意义（P＞0.05）；STAT3阳性表达与肿瘤直径、是否伴有颈部淋巴结转移相关（P＜0.05），而与性别、年龄、病灶数、是否侵犯甲状腺外被膜、是否合并桥本甲状腺炎无关（P＞0.05）；NCK1阳性表达与是否伴有颈部淋巴结转移相关（P＜0.05），而与性别、年龄、肿瘤直径、病灶数、是否侵犯甲状腺外被膜、是否合并桥本甲状腺炎无关（P＞0.05）；VEGF阳性表达与肿瘤直径、是否侵犯甲状腺外被膜和是否伴有颈部淋巴结转移相关（P＜0.05），而与性别、年龄、病灶数、是否合并桥本甲状腺炎无关（P＞0.05）。Spearman秩相关性分析显示，甲状腺乳头状癌中STAT3与NCK1的表达呈正相关（r=0.483，P＜0.05）；STAT3与VEGF的表达呈正相关（r=0.584，P＜0.05）；NCK1与VEGF的表达呈正相关（r=0.477，P＜0.05）。结论 STAT3、NCK1、VEGF存在协同作用，共同促进了甲状腺乳头状癌的血管生成和颈部淋巴结转移过程。
Abstract:
Objective To study the expression of STAT3, NCK1 and VEGF in papillary thyroid carcinoma and its clinical significance.Methods Sixty-three cases of papillary thyroid carcinoma, 32 cases of thyroid adenoma, and 20 cases of normal thyroid tissue were collected from September 2019 to December 2020 in Tangshan Workers’ Hospital. The expression levels of STAT3, NCK1 and VEGF were detected by immunohistochemistry. The expression of STAT3, NCK1 and VEGF in different thyroid tissues was analyzed, and the relationship between STAT3, NCK1 and VEGF in papillary thyroid carcinoma and clinical pathology was analyzed.Results The positive expression rates of STAT3, NCK1 and VEGF in papillary thyroid carcinoma were higher than those in thyroid adenoma and normal thyroid tissue, and the difference was statistically significant (P<0.05); while there was no significant difference in the positive expression rates of STAT3, NCK1 and VEGF between thyroid adenoma and normal thyroid tissues (P>0.05). STAT3 positive expression was correlated with tumor diameter and cervical lymph node metastasis (P<0.05), while was not related to gender, age, number of lesions, invasion of thyroid envelope, and hashimoto thyroiditis (P>0.05). The positive expression of NCK1 was correlated with cervical lymph node metastasis (P<0.05), while was not related to gender, age, tumor diameter, number of lesions, invasion of thyroid envelope, and hashimoto thyroiditis (P>0.05). The positive expression of VEGF was correlated with tumor diameter, invasion of thyroid envelope and cervical lymph node metastasis (P<0.05), was no correlation with gender, age, number of lesions and hashimoto thyroiditis (P>0.05). Spearman rank correlation analysis showed that STAT3 was positively correlated with NCK1 expression in papillary thyroid carcinoma (r=0.483, P<0.05); STAT3 was positively correlated with VEGF expression (r=0.584, P<0.05); NCK1 was positively correlated with VEGF expression (r=0.477, P<0.05).Conclusion STAT3, NCK1 and VEGF have a synergistic effect, which together promote the angiogenesis and cervical lymph node metastasis process of thyroid papillary carcinoma.

参考文献/References:

[1]《免疫组织化学检测技术共识》编写组.免疫组织化学检测技术共识[J].中华病理学杂志,2019,48(2):87-91.[2]Maeda-Otsuka S,Kajihara I,Tasaki Y,et al.Hypoxia accelerates the progression of angiosarcoma through the regulation of angiosarcoma cells and tumor microenvironment[J].J Dermatol Sci,2019,93(2):123-132.[3]Wang HF,Wang SS,Zheng M,et al.Hypoxia promotes vasculogenic mimicry formation by vascular endothelial growth factor A mediating epithelial-mesenchymal transition in salivary adenoid cystic carcinoma[J].Cell Prolif,2019,52(3):e12600. [4]Hong YM,Gan WG,Xu ZH.Significance of the expression of integrin β1, VEGF and MVD in hypopharyngeal squamous cell carcinoma[J].Genet Mol Res,2014,13(3):6455-6465. [5]Aggarwal S,Devaraja K,Sharma SC,et al.Expression of vascular endothelial growth factor (VEGF) in patients with oral squamous cell carcinoma and its clinical significance[J].Clin Chim Acta,2014(436):35-40.[6]Selemetjev S,Doric I,Paunovic I,et al.Coexpressed High Levels of VEGF-C and Active MMP-9 Are Associated With Lymphatic Spreading and Local Invasiveness of Papillary Thyroid Carcinoma[J].Am J Clin Pathol,2016,146(5):594-602.[7]郭秉楠,燕宪亮,许铁.缺氧对骨髓源性抑制性细胞向肿瘤相关巨噬细胞分化的影响[J].江苏医药,2020,46(11):1094-1097,1101.[8]Pang L,Wang J,Fan Y,et al.Correlations of TNM staging and lymph node metastasis of gastric cancer with MRI features and VEGF expression[J].Cancer Biomark,2018,23(1):53-59. [9]Salajegheh A,Pakneshan S,Rahman A,et al.Co-regulatory potential of vascular endothelial growth factor-A and vascular endothelial growth factor-C in thyroid carcinoma[J].Hum Pathol,2013,44(10):2204-2212.[10]Fanelli M,Camperchioli A,Petrella L,et al.Non-catalytic region of tyrosine kinase adaptor protein 2 (NCK2) pathways as factor promoting aggressiveness in ovarian cancer[J].Int J Biol Markers,2018,33(1):124-131.[11]Lettau M,Kliche S,Kabelitz D,et al.The adapter proteins ADAP and Nck cooperate in T cell adhesion[J].Mol Immunol,2014,60(1):72-79.[12]Alfaidi M,Bhattarai U,Orr AW.Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability[J].J Am Heart Assoc,2020,9(11):e016099.[13]Morris DC,Popp JL,Tang LK,et al.Nck deficiency is associated with delayed breast carcinoma progression and reduced metastasis[J].Mol Biol Cell,2017,28(24):3500-3516.[14]Xia P,Huang M,Zhang Y,et al.NCK1 promotes the angiogenesis of cervical squamous carcinoma via Rac1/PAK1/MMP2 signal pathway[J].Gynecol Oncol,2019,152(2):387-395.[15]Mohassab AM,Hassan HA,Abdelhamid D,et al.STAT3 transcription factor as target for anti-cancer therapy[J].Pharmacol Rep,2020,72(5):1101-1124.[16]王亦如,杨梦莹,阳志军.基于生物信息学分析STAT3、Grim19在卵巢癌中的表达及临床意义[J].华中科技大学学报(医学版),2020,49(3):266-274.[17]伍婧,邓伟英,胡斌,等.STAT3与VEGF蛋白在结肠癌中的表达及对血管生成的影响[J].中国临床研究,2017(2):208-210.[18]韩婧,康骅.甲状腺癌的发病现状及影响因素[J].实用预防医学,2018,25(7):894-897.[19]中华人民共和国国家卫生健康委员会.甲状腺癌诊疗规范(2018年版)[J].中华普通外科学文献(电子版),2019,13(1):1-15.[20]Zhang F,Lu YX,Chen Q,et al.Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis[J].Cell Signal,2017(36):67-78.

更新日期/Last Update: 1900-01-01