[1]张道宝,徐建国.miR-200c对颅咽管瘤侵袭性的影响及作用机制[J].医学信息,2023,36(02):54-60.[doi:10.3969/j.issn.1006-1959.2023.02.011]
　ZHANG Dao-bao,XU Jian-guo.The Effect and Mechanism of miR-200c on the Invasiveness of Craniopharyngioma[J].Journal of Medical Information,2023,36(02):54-60.[doi:10.3969/j.issn.1006-1959.2023.02.011]
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miR-200c对颅咽管瘤侵袭性的影响及作用机制()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年02期
页码:
54-60
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
The Effect and Mechanism of miR-200c on the Invasiveness of Craniopharyngioma
文章编号:
1006-1959（2023）02-0054-07
作者:
张道宝; 徐建国
（1.四川大学华西医院神经外科，四川 成都 610000；2.乐山市人民医院脑血管病科，四川 乐山 614000）
Author(s):
ZHANG Dao-bao; XU Jian-guo
(1.Department of Neurosurgery,West China Hospital of Sichuan University,Chengdu 610000,Sichuan,China;2.Department of Cerebrovascular Disease,Leshan People’s Hospital,Leshan 614000,Sichuan,China)
关键词:
颅咽管瘤; 侵袭性; miRNA-200c; E-cadherin; β-catenin
Keywords:
Craniopharyngioma; Invasive; miRNA-200c; E-cadherin; β-catenin
分类号:
R739.41
DOI:
10.3969/j.issn.1006-1959.2023.02.011
文献标志码:
A
摘要:
目的 探讨miR-200c对颅咽管瘤侵袭性的影响，并阐述其作用机制。方法 选择造釉细胞型及鳞状上皮乳头型颅咽管瘤原代细胞为对象，研究miR-200c在原代细胞中的表达情况。通过对造釉细胞型颅咽管瘤原代细胞过表达和敲低miR-200c，研究miR-200c对造釉细胞型颅咽管瘤侵袭性的影响及作用机制。结果 与鳞状上皮乳头型颅咽管瘤原代细胞组相比，造釉细胞型颅咽管瘤原代细胞组中miRNA-200c的表达下降，统计学意义极显著（P＜0.001）；与对照组相比，miR-200c mimics组结晶紫染色细胞数量减少（P＜0.05），而miR-200c inhibitor组结晶紫染色细胞数量增加（P＜0.05），miR-200c inhibitor组细胞数多于miR-200c mimics组，统计学意义显著（P＜0.01）。结论 miRNA-200c在造釉细胞型颅咽管瘤原代细胞中表达明显减少，促进了EMT的发生，导致造釉细胞型颅咽管瘤的侵袭性增加。
Abstract:
Objective To explore the effect of miR-200c on the invasiveness of craniopharyngioma and clarify its mechanism of action.Methods The primary cells of adamantinomatous craniopharyngiomas and papillary craniopharyngiomas were selected to study the expression of miR-200c in primary cells. Through overexpression and knockdown of miR-200c in primary cells of adamantinomatous craniopharyngiomas, the effect of miR-200c on the invasiveness of adamantinomatous craniopharyngiomas and its mechanism were studied.Results Compared with the primary cell group of squamous papillary craniopharyngioma, the expression of miRNA-200c in the primary cell group of adamantinomatous craniopharyngioma decreased significantly (P<0.001). Compared with the control group, the number of crystal violet staining cells in the miR-200c mimics group decreased (P<0.05), while the number of crystal violet staining cells in the miR-200c inhibitor group increased (P<0.05), and the number of cells in the miR-200c inhibitor group was more than that in the miR-200c mimics group (P<0.01).Conclusion The expression of miRNA-200c in primary cells of adamantinomatous craniopharyngioma is significantly reduced, which promotes the occurrence of EMT and increased the invasiveness of adamantinomatous craniopharyngioma.

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更新日期/Last Update: 1900-01-01