[1]严子璇,艾 瑛,陈明庆,等.机械损伤构建大鼠宫腔粘连模型效果分析[J].医学信息,2023,36(02):61-64.[doi:10.3969/j.issn.1006-1959.2023.02.012]
　YAN Zi-xuan,AI Ying,CHEN Ming-qing,et al.Effect Analysis of Rat Intrauterine Adhesion Model Constructed by Mechanical Injury[J].Journal of Medical Information,2023,36(02):61-64.[doi:10.3969/j.issn.1006-1959.2023.02.012]
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机械损伤构建大鼠宫腔粘连模型效果分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年02期
页码:
61-64
栏目:
论著
出版日期:
2023-01-15

文章信息/Info

Title:
Effect Analysis of Rat Intrauterine Adhesion Model Constructed by Mechanical Injury
文章编号:
1006-1959（2023）02-0061-04
作者:
严子璇; 艾 瑛; 陈明庆; 等
（1.昆明理工大学医学院，云南 昆明 650032；2.云南省第一人民医院/昆明理工大学附属医院妇产科，云南 昆明 650032）
Author(s):
YAN Zi-xuan; AI Ying; CHEN Ming-qing; et al.
(1.School of Medicine,Kunming University of Science and Technology,Kunming 650032,Yunnan,China;2.Department of Obstetrics and Gynecology,the First People’s Hospital of Yunnan Province/the Affiliated Hospital ofKunming University of Science and Technolog
关键词:
宫腔粘连; 机械刮宫; 动物模型; 子宫内膜形态
Keywords:
Intrauterine adhesion; Mechanical curettage; Animal model; Endometrial pathologic changes
分类号:
R711
DOI:
10.3969/j.issn.1006-1959.2023.02.012
文献标志码:
A
摘要:
目的 通过分析机械损伤构建大鼠宫腔粘连模型的效果，为进一步揭示宫腔粘连的发病机制及临床治疗提供依据。方法 采用机械刮宫法，将麻醉后的未受孕雌性SD大鼠一侧子宫刮宫，对侧切开暴露内膜进行自身对照，构建大鼠宫腔粘连模型。在建模后的不同时间点取大鼠子宫标本，对标本进行大体比较，常规固定后HE染色、Masson染色观察组织形态，并用Western Blot技术检测大鼠子宫刮宫段TGF-β1的表达水平，判定不同时间点大鼠宫腔粘连模型效果。结果 与对照组相比，刮宫组子宫直径明显增粗，宫腔表面可见环形狭窄，宫腔局部或整体粘连阻塞；刮宫组子宫内膜厚度薄于对照组、子宫内膜腺体数量少于对照组、子宫内膜纤维化面积比率高于对照组（P＜0.05）；建模后宫腔粘连在第3天到达高峰，第5天后开始修复；刮宫后第3天，TGF-β1表达水平提高，宫腔粘连程度最为明显。结论 大鼠宫腔粘连模型可以通过机械刮宫构建，模型建立后子宫内膜变薄、子宫内膜腺体数量减少、子宫内膜纤维化面积增加，与人类宫腔粘连病理情况相似，且建模后第3天，受损子宫TGF-β1的表达量最高，大鼠子宫宫腔粘连程度最为严重。
Abstract:
Objective To analyze the effect of mechanical injury on the construction of rat model of intrauterine adhesions, and to provide a basis for further revealing the pathogenesis and clinical treatment of intrauterine adhesions.Methods The mechanical curettage method was used to curettage one side of the uterus of unpregnant female SD rats after anesthesia, and the opposite side was cut to expose the endometrium for self-control to construct a rat intrauterine adhesion model. The uterine specimens of rats were taken at different time points after modeling, and the specimens were compared generally. After routine fixation, HE staining and Masson staining were used to observe the tissue morphology, and the expression level of TGF-β1 in the uterine curettage section of rats was detected by Western Blot technique to determine the effect of rat intrauterine adhesion model at different time points.Results Compared with the control group, the uterine diameter of the curettage group was significantly thicker, the surface of the uterine cavity was seen as annular stenosis, and the local or overall adhesion of the uterine cavity was blocked. The endometrial thickness of the curettage group was thinner than that of the control group, the number of endometrial glands was less than that of the control group, and the ratio of endometrial fibrosis area was higher than that of the control group (P<0.05). After modeling, intrauterine adhesions reached a peak on the third day and began to repair after the fifth day. On the third day after curettage, the expression level of TGF-β1 increased, and the degree of intrauterine adhesion was the most obvious.Conclusion The rat model of intrauterine adhesions can be constructed by mechanical curettage. After the model is established, the endometrium becomes thinner, the number of endometrial glands decreases, and the area of endometrial fibrosis increases, which is similar to the pathological condition of human intrauterine adhesions. On the third day after modeling, the expression of TGF-β1 in the damaged uterus is the highest, and the degree of intrauterine adhesions in rats is the most serious.

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更新日期/Last Update: 1900-01-01