[1]张永芹,左 云,王少开,等.盐霉素联合X射线照射对食管癌ECA109及TE13细胞增殖及细胞周期的影响[J].医学信息,2023,36(07):90-95.[doi:10.3969/j.issn.1006-1959.2023.07.016]
　ZHANG Yong-qin,ZUO Yun,WANG Shao-kai,et al.Effect of Salinomycin Combined with X-irradiation on Proliferation and Cell Cycle of Esophageal Cancer ECA109 and TE13 Cells[J].Journal of Medical Information,2023,36(07):90-95.[doi:10.3969/j.issn.1006-1959.2023.07.016]
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盐霉素联合X射线照射对食管癌ECA109及TE13细胞增殖及细胞周期的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年07期
页码:
90-95
栏目:
论著
出版日期:
2023-04-01

文章信息/Info

Title:
Effect of Salinomycin Combined with X-irradiation on Proliferation and Cell Cycle of Esophageal Cancer ECA109 and TE13 Cells
文章编号:
1006-1959（2023）07-0090-06
作者:
张永芹; 左 云; 王少开; 等
（苏州大学附属张家港医院肿瘤科，江苏 苏州 215600）
Author(s):
ZHANG Yong-qin; ZUO Yun; WANG Shao-kai; et al.
(Department of Oncology,the Affiliated Zhangjiagang Hospital of Soochow University,Suzhou 215600,Jiangsu,China)
关键词:
盐霉素; 食管癌; 增殖抑制; 细胞周期
Keywords:
Salinomycin; Esophageal cancer; Proliferation inhibition; Cell cycle
分类号:
R735.1
DOI:
10.3969/j.issn.1006-1959.2023.07.016
文献标志码:
A
摘要:
目的 研究盐霉素联合X射线照射对人食管癌ECA109及TE13细胞增殖及细胞周期的影响。方法 取对数生长期的ECA109及TE13细胞，将其分为对照组、不同浓度盐霉素组、6 Gy X射线照射组以及不同浓度盐霉素联合6 Gy X射线照射组。在干预后24、48 h后，采用CCK-8法检测各组细胞增殖率。然后取对数生长期的ECA109及TE13细胞，将其分为对照组、0.1 μmol/L盐霉素组、0.5 μmol/L盐霉素组、6 Gy X射线照射组和0.1 μmol/L盐霉素联合6 Gy X射线照射组、0.5 μmol/L盐霉素联合6 Gy X射线照射组，采用流式细胞术检测各组ECA109及TE13细胞的细胞周期。结果 0.1、0.25、0.5、1、2.5、5、10、50、100 μmol/L盐霉素组干预24、48 h后ECA109细胞及TE13细胞增殖率低于对照组（P＜0.05）；0.1、0.5 μmol/L盐霉素联合6 Gy X射线照射组干预24、48 h后ECA109及TE13细胞增殖率均低于6 Gy X射线照射组（P＜0.05）；0.1、0.5 μmol/L盐霉素联合6 Gy X射线照射组ECA109细胞及TE13细胞G0/G1期细胞比例低于对照组、0.1 μmol/L盐霉素组、0.5 μmol/L盐霉素组及6 Gy X射线照射组，0.1、0.5 μmol/L盐霉素联合6 Gy X射线照射组ECA109细胞及TE13细胞G2/M期比例高于对照组、0.1 μmol/L盐霉素组、0.5 μmol/L盐霉素组及6 Gy X射线照射组（P＜0.05）。结论 盐霉素对ECA109及TE13细胞均具有增殖抑制作用，且呈浓度和时间依赖性。盐霉素和X射线联合作用的抑制效果明显优于二者单独作用，其机制可能与盐霉素联合射线联合引起ECA109及TE13细胞G2/M期阻滞有关。
Abstract:
Objective To study the effect of salinomycin combined with X-ray irradiation on the proliferation and cell cycle of human esophageal cancer ECA109 and TE13 cells.Methods ECA109 and TE13 cells in logarithmic growth period were divided into control group, salinomycingroup of different concentrations, 6 Gy X-ray irradiation group and salinomycin combined with 6 Gy X-ray irradiation group. CCK-8 method was used to detect the cell proliferation rates in various groups at 24 and 48 h after treatment. ECA109 and TE13 cells in logarithmic growth phase were divided into control group, 0.1 μmol/L salinomycin group, 0.5 μmol/L salinomycin group, 6 Gy X-ray irradiation group, 0.1 μmol/L salinomycin combined with 6 Gy X-ray irradiation group and 0.5 μmol/L salinomycin combined with 6 Gy X-ray irradiation group. The cell cycle percentage of ECA109 and TE13 cells in each group was detected by flow cytometry.Results The proliferation rates of ECA109 cells and TE13 cells in 0.1, 0.25, 0.5, 1, 2.5, 5, 10, 50 and 100 μmol/L salinomycin groups were lower than those in control group at 24, 48 h after intervention (P<0.05). The proliferation rates of ECA109 and TE13 cells in the 0.1 and 0.5 μmol/L salinomycin combined with 6 Gy X-ray irradiation groups were lower than those in the 6 Gy X-ray irradiation group after 24 and 48 h of intervention (P<0.05). The proportion of G0/G1 phase cells in ECA109 cells and TE13 cells in 0.1 and 0.5 μmol/L salinomycin combined with 6 Gy X-ray irradiation groups was lower than that in control group, 0.1 μmol/L salinomycin group, 0.5 μmol/L salinomycin group and 6 Gy X-ray irradiation group; the proportions of ECA109 cells and TE13 cells in G2/M phase in 0.1 and 0.5 μmol/L salinomycin combined with 6 Gy X-ray irradiation groups were higher than those in control group, 0.1 μmol/L salinomycin group, 0.5 μmol/L salinomycin group and 6 Gy X-ray irradiation group (P<0.05).Conclusion Salinomycin can inhibit the proliferation of ECA109 and TE13 cells in a concentration-dependent and time-dependent manner, and the combined effect of salinomycin and X-ray is better than that of the two alone, the mechanism may be related to the G2/M phase arrest of ECA109 and TE13 cells caused by the combination of salinomycin and X-ray.

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更新日期/Last Update: 1900-01-01