[1]岳宇娇,徐 平.新生大鼠海马神经元原代细胞培养方法改良及鉴定[J].医学信息,2023,36(17):111-114.[doi:10.3969/j.issn.1006-1959.2023.17.021]
　YUE Yu-jiao,XU Ping.Improvement and Identification of Primary Cell Culture Method of Neonatal Rat Hippocampal Neurons[J].Journal of Medical Information,2023,36(17):111-114.[doi:10.3969/j.issn.1006-1959.2023.17.021]
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新生大鼠海马神经元原代细胞培养方法改良及鉴定()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年17期
页码:
111-114
栏目:
论著
出版日期:
2023-09-01

文章信息/Info

Title:
Improvement and Identification of Primary Cell Culture Method of Neonatal Rat Hippocampal Neurons
文章编号:
1006-1959（2023）17-0111-04
作者:
岳宇娇; 徐 平
（遵义医科大学附属医院神经康复科1，神经内科2，贵州 遵义 563000）
Author(s):
YUE Yu-jiao; XU Ping
(Department of Neuro-rehabilitation1,Department of Neurological2,Affiliated Hospital of Zunyi Medical University,Zunyi 563000,Guizhou,China)
关键词:
海马; 神经元; 原代; 培养; 微管相关蛋白2
Keywords:
Hippocampus; Neuron; Primary; Culture; Microtubule associated protein 2
分类号:
R741
DOI:
10.3969/j.issn.1006-1959.2023.17.021
文献标志码:
A
摘要:
目的 探索一种稳定、经济、实用的SD新生鼠海马神经元的原代培养方法。方法 选用出生24 h内的SD新生鼠，分离新生大鼠双侧海马组织制备海马组织单细胞悬液，采用DMEM/F12+2%B27培养体系维持培养，观察海马神经元形态变化，取培养5 d的海马神经元原代培养细胞，采用微管相关蛋白2（MAP2）免疫荧光染色鉴定神经元纯度。结果 提取的海马神经元细胞存活率高，生长状态良好；MAP2免疫荧光鉴定神经元纯度达（95.20±1.50）%。结论 本培养方法在保证海马神经元细胞纯度及细胞活性的同时，降低原代神经元培养成本，是一种稳定、经济、实用的海马神经元原代培养方法。关键词：海马；神经元；原代；培养；微管相关蛋白2
Abstract:
Objective To explore a stable, economical and practical primary culture method of hippocampal neurons in SD neonatal rats.Methods SD neonatal rats within 24 hours after birth were selected. The bilateral hippocampal tissues of neonatal rats were isolated to prepare single cell suspension of hippocampal tissues. The DMEM/F12+2%B27 culture system was used to maintain culture. The morphological changes of hippocampal neurons were observed. The primary cultured cells of hippocampal neurons cultured for 5 days were taken, and the purity of neurons was identified by microtubule-associated protein 2 (MAP2) immunofluorescence staining.Results The extracted hippocampal neuron cells had high survival rate and good growth state. The purity of neurons identified by MAP2 immunofluorescence was (95.20±1.50)%.Conclusion This method can not only ensure the purity and activity of hippocampal neurons, but also reduce the cost of primary neuron culture. It is a stable, economic and practical primary culture method of hippocampal neurons.

参考文献/References:

[1]Barbiellini Amidei C,Fayosse A,Dumurgier J,et al.Association between age at diabetes onset and subsequent risk of dementia[J].JAMA,2021,325(16):1640-1649.[2]The Lancet N.Better care for people living with dementia in the USA[J].Lancet Neurol,2021,20(5):327.[3]Hendriks S,Peetoom K,Bakker C,et al.Global prevalence of young-onset dementia: A systematic review and meta-analysis[J].JAMA Neurol,2021,78(9):1080-1090.[4]康晨瑶,刘洲,周海红.Nlrp3炎症小体在AD发病机制中的作用[J].医学信息,2021,34(12):40-43.[5]Roos TT,Garcia MG,Martinsson I,et al.Neuronal spreading and plaque induction of intracellular aβ and its disruption of aβ homeostasis[J].Acta Neuropathol,2021,142(4):669-687.[6]Gibbons GS,Kim SJ,Wu Q,et al.Conformation-selective tau monoclonal antibodies inhibit tau pathology in primary neurons and a mouse model of alzheimer’s disease[J].Mol Neurodegener,2020,15(1):64.[7]Hammoud H,Netsyk O,Tafreshiha AS,et al.Insulin differentially modulates gaba signalling in hippocampal neurons and, in an age-dependent manner, normalizes gaba-activated currents in the tg-appswe mouse model of alzheimer’s disease[J].Acta Physiol (Oxf),2021,232(2):e13623.[8]Kragel JE,Voss JL.Looking for the neural basis of memory[J].Trends Cogn Sci,2022,26(1):53-65.[9]Hasan MF,Ghiasvand S,Wang H,et al.Neural layer self-assembly in geometrically confined rat and human 3d cultures[J].Biofabrication,2019,11(4):045011.[10]Hornsby AKE,Buntwal L,Carisi MC,et al.Unacylated-ghrelin impairs hippocampal neurogenesis and memory in mice and is altered in parkinson’s dementia in humans[J].Cell Rep Med,2020,1(7):100120.[11]Volpicelli-Daley LA,Luk KC,Lee VM.Addition of exogenous α-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous α-synuclein to lewy body and lewy neurite-like aggregates[J].Nat Protoc,2014,9(9):2135-2146.[12]Wei Q,Chen J,Xiao F,et al.High-dose dexmedetomidine promotes apoptosis in fetal rat hippocampal neurons[J].Drug Des Devel Ther,2021,15:2433-2444.[13]Li S,Raychaudhuri S,Lee SA,et al.Asynchronous release sites align with nmda receptors in mouse hippocampal synapses[J].Nat Commun,2021,12(1):677.[14]Ye L,Ji H,Liu J,et al.Carbon nanotube-hydrogel composites facilitate neuronal differentiation while maintaining homeostasis of network activity[J].Adv Mater,2021,33(41):e2102981.[15]仁德芳,付裕,王洪连,等.乳鼠海马神经元的分离和原代培养[J].细胞与分子免疫学杂志,2017,33(5):660-663.[16]冯龙,刘蔷薇,冯泽国,等.改良无血清法培养新生SD乳鼠原代海马神经元细胞[J].解放军医学院学报,2020,41(12):1222-1225.[17]张余,刘英富,陈旭义,等.阿糖胞苷对大鼠海马神经元原代培养的影响[J].中国应用生理学杂志,2016,32(3):218-220,224.[18]李梨,吴芹,蒋青松,等.原代培养神经元拟缺血性损伤模型的建立[J].遵义医学院学报,2004,27(5):441-443[19]金正旭,丁韵涵,王小娟,等.新生大鼠海马神经元的分离培养及鉴定[J].兰州大学学报(医学版),2020,46(3):20-22,27.[20]Sánchez C,Díaz-Nido J,Avila J.Phosphorylation of microtubule-associated protein 2 (map2) and its relevance for the regulation of the neuronal cytoskeleton function[J].Prog Neurobiol,2000,61(2):133-168.[21]Ribeiro LF,de Wit J.Neuronal polarity: Map2 shifts secretory vesicles into high gear for long-haul transport down the axon[J].Neuron,2017,94(2):223-225.

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更新日期/Last Update: 1900-01-01