[1]李 伟,范 磊,肖 娟,等.先天性MCMV感染对小鼠海马中胆固醇表达的影响及意义[J].医学信息,2018,31(18):45-48.[doi:10.3969/j.issn.1006-1959.2018.18.015]
　LI Wei,FAN Lei,XIAO Juan,et al.Effect and Significance of Congenital MCMV Infection on Cholesterol Expression in Hippocampus of Mice[J].Journal of Medical Information,2018,31(18):45-48.[doi:10.3969/j.issn.1006-1959.2018.18.015]
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先天性MCMV感染对小鼠海马中胆固醇表达的影响及意义()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年18期
页码:
45-48
栏目:
论著
出版日期:
2018-09-15

文章信息/Info

Title:
Effect and Significance of Congenital MCMV Infection on Cholesterol Expression in Hippocampus of Mice
文章编号:
1006-1959(2018)18-0045-04
作者:
李 伟; 范 磊; 肖 娟; 刘 静; 曾 雪; 陈素华; 乌剑利
华中科技大学同济医学院附属同济医院妇产科，湖北 武汉 430030
Author(s):
LI Wei; FAN Lei; XIAO Juan; LIU Jing; ZENG Xue; CHEN Su-hua; WU Jian-li
Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030,Hubei,China
关键词:
高效液相色谱; 巨细胞病毒; 海马; 胆固醇
Keywords:
Key words：High performance liquid chromatography; Cytomegalovirus; Hippocampus; Cholesterol
分类号:
R654.4
DOI:
10.3969/j.issn.1006-1959.2018.18.015
文献标志码:
A
摘要:
目的 观察先天性MCMV神经系统感染对小鼠海马发育过程中胆固醇表达的影响。方法 建立先天性MCMV神经系统感染模型，用HPLC法检测发育3日龄、15日龄及30日龄小鼠海马胆固醇表达水平，并与对照组相比较。结果 在先天性MCMV感染组及对照组中，海马胆固醇表达水平均呈渐进性增加，差异统计学意义显著（P＜0.01）；与对照组比较，MCMV感染组海马表达水平均低于同时期对照组，差异统计学意义显著（P＜0.01）。结论 胆固醇在海马发育过程中起重要作用，先天性MCMV感染能在近期、中远期降低海马胆固醇表达水平，这可能是CMV宫内感染导致子代出现中远期神经系统后遗症的发病机制之一。
Abstract:
Abstract：Objective To observe the effect of congenital MCMV nervous system infection on cholesterol expression during hippocampus development in mice.Methods A congenital MCMV nervous system infection model was established.The expression of cholesterol in hippocampus of mice at 3 days,15 days and 30 days old was detected by HPLC and compared with the control group. Results In the congenital MCMV infection group and the control group,the expression level of cholesterol in hippocampus increased gradually,the difference was statistically significant(P<0.01).Compared with the control group,the expression level of hippocampus in MCMV infection group was lower than that in the control group,the difference was statistically significant(P<0.01).Conclusion Cholesterol plays an important role in the development of hippocampus.Congenital MCMV infection can reduce hippocampal cholesterol expression in the short-term and medium-term, which may be one of the pathogenesis of medium-term and long-term neurological sequelae in CMV intrauterine infection.

参考文献/References:


[1]Lim Y,Lyall H.Congenital cytomegalovirus who,when,what-with and why to treat[J].J Infect.2017,74 Suppl 1(1):S89-S94.
[2]Inoue N,Abe M,Kobayashi R,et al.Vaccine Development for Cytomegalovirus[J].Adv Exp Med Biol.2018,1045:271-296.
[3]Capretti MG,Marsico C,Guidelli Guidi S,et al.Neonatal and long-term ophthalmological findings in infants with symptomatic and asymptomatic congenital cytomegalovirus infection[J].J Clin Virol,2017,97(11):59-63.
[4]Schleiss MR.Congenital Cytomegalovirus Infection:Improved Understanding of Maternal Immune Responses That Reduce the Risk of Transplacental Transmission[J].Clin Infect Dis.2017,65 (10):1666-1669.
[5]Fowler KB,Boppana SB.Congenital cytomegalovirus infection[J].Semin Perinatol,2018,42 (3):149-154.
[6]Fukui K,Ferris HA,Kahn CR.Effect of cholesterol reduction on receptor signaling in neurons[J].J Biol Chem.2015,290(44):26383-92.
[7]Antonini A,Caioli S,Saba L,et al.Membrane cholesterol depletion in cortical neurons highlights altered NMDA receptor functionality in a mouse model of amyotrophic lateral sclerosis[J].Biochim Biophys Acta,2018,1864(2):509-519.
[8]Frank C,Rufini S.Cholesterol depletion inhibits synaptic transmission and synaptic plasticity in rat hippocampus[J].Exp Neurol.2008,212(2):407-14.
[9]李伟,熊锦文,陈娟娟,等.金叶败毒颗粒防治妊娠中期巨细胞病毒宫内感染的实验研究[J].医药导报,2015,34(5):585-588.
[10]Bantug GR,Cekinovic D,Bradford R,et al.CD8+T lymphocytes control murine cytomegalovirus replication in the central nervous system of newborn animals [J].2008,181(3):2111-23.
[11]Chali F,Djelti F,Eugene E,et al.Inhibiting cholesterol degradation induces neuronal sclerosis and epileptic activity in mouse hippocampus[J].Eur J Neurosci,2015,41(10):1345-55.
[12]Fester L,Zhou L,Bütow A,et al.Cholesterol-promoted synaptogenesis requires the conversion of cholesterol to estradiol in the hippocampus[J].Hippocampus.2009,19(8):692-705.
[13]Slavuljica I,Kvetak D,Huszthy PC,et al.Immunobiology of congenital cytomegalovirus infection of the central nervous system-the murine cytomegalovirus model[J].2015,12(2):180-91.
[14]Laukoter S,Rauschka H,Trscher AR,et al.Differences in T cell cytotoxicity and cell death mechanisms between progressive multifocal leukoencephalopathy,herpes simplex virus encephalitis and cytomegalovirus encephalitis[J].Acta Neuropathol.2017,133(4):613-627.

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更新日期/Last Update: 2018-09-15