[1]王 琳,王笔金.儿童支原体大叶性肺炎三种病原体检测方法的对比分析[J].医学信息,2024,37(18):83-87.[doi:10.3969/j.issn.1006-1959.2024.18.015]
　WANG Lin,WANG Bi-jin.Comparative Analysis of Three Pathogen Detection Methods for Mycoplasma Lobar Pneumonia in Children[J].Journal of Medical Information,2024,37(18):83-87.[doi:10.3969/j.issn.1006-1959.2024.18.015]
点击复制

儿童支原体大叶性肺炎三种病原体检测方法的对比分析()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
37卷
期数:
2024年18期
页码:
83-87
栏目:
论著
出版日期:
2024-09-15

文章信息/Info

Title:
Comparative Analysis of Three Pathogen Detection Methods for Mycoplasma Lobar Pneumonia in Children
文章编号:
1006-1959（2024）18-0083-05
作者:
王 琳1; 王笔金2
南京医科大学第二附属医院检验医学中心1，输血科2，江苏 南京 210011
Author(s):
WANG Lin1; WANG Bi-jin2
Laboratory Medicine Center1,Blood Transfusion Department2,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,Jiangsu,China
关键词:
肺炎支原体; 大叶性肺炎; 肺炎支原体DNA; 肺炎支原体抗体
Keywords:
Mycoplasma pneumonia; Lobar pneumonia; Mycoplasma pneumoniae DNA; Mycoplasma pneumoniae antibody
分类号:
R725.6
DOI:
10.3969/j.issn.1006-1959.2024.18.015
摘要:
目的 研究肺炎支原体感染所致大叶性肺炎的早期实验室检查结果，探讨相关指标的疾病预测价值。方法 回顾性分析南京医科大学第二附属医院2023年1月-8月收治的疑似肺炎支原体感染致大叶性肺炎患儿的实验室检测结果，分析三种支原体病原体和血清学检测方法对儿童支原体大叶性肺炎的早期诊断意义。结果 核酸检测法的MP阳性检出率最高，化学发光法、间接免疫荧光法和核酸检测法的MP检测阳性率分别为37.78%、28.89%和82.22%；三种检测方法的一致性较差，两两比较的Kappa值分别为0.089、-0.444和-0.533。不同性别患儿化学发光法MP检测结果比较，差异有统计学意义（P＜0.05）；不同年龄、不同拷贝数三种检测方法MP阳性检出率比较，差异无统计学意义（P＞0.05）。结论 同步进行核酸和抗体检测有助于及早、准确诊断儿童支原体肺炎，为该疾病的早期诊断和治疗提供依据。
Abstract:
Objective To study the early laboratory examination results of lobar pneumonia caused by Mycoplasma pneumoniae infection, and to explore the predictive value of related indicators.Methods The laboratory test results of children with lobar pneumonia caused by suspected mycoplasma pneumoniae infection admitted to the Second Affiliated Hospital of Nanjing Medical University from January to August 2023 were retrospectively analyzed. The significance of three mycoplasma pathogens and serological detection methods in the early diagnosis of mycoplasma lobar pneumonia in children was analyzed.Results The positive detection rate of MP by nucleic acid detection method was the highest, the positive detection rates of MP by chemiluminescence method, indirect immunofluorescence method and nucleic acid detection method were 37.78%, 28.89% and 82.22%, respectively, the consistency of the three detection methods was poor, and the Kappa values of pairwise comparison were 0.089, -0.444 and-0.533, respectively. There was a statistically significant difference in the results of MP detection by chemiluminescence in children of different genders (P<0.05).Conclusion Simultaneous nucleic acid and antibody detection is helpful for early and accurate diagnosis of mycoplasma pneumonia in children, and provides a basis for early diagnosis and treatment of the disease.

参考文献/References:

[1]中华人民共和国国家健康委员会,国家中医药局.儿童社区获得性肺炎诊疗规范(2019年版)[J].中华临床感染病杂志,2019,12(1):6-13.[2]Liu WK,Liu Q,Chen DH,et al.Epidemiology of acute respiratory infections in children in Guangzhou: a three-year study[J].PLoS One,2014,9(5):e96674.[3]李素荣,牟京辉,常丽,等.肺炎支原体感染所致儿童坏死性肺炎30例胸部CT表现及转归[J].中华儿科杂志,2013,51(3):211-215.[4]Yun KW,Wallihan R,Juergensen A,et al.Community-Acquired Pneumonia in Children: Myths and Facts[J].Am J Perinatol,2019,36(S02):S54-S57.[5]Zhang C,Zhang Q,Du JL,et al.Correlation Between the Clinical Severity, Bacterial Load, and Inflammatory Reaction in Children with Mycoplasma Pneumoniae Pneumonia[J].Curr Med Sci,2020,40(5):822-828.[6]Su M,Wang Q,Li D,et al.Prevalence and clinical characteristics of hospitalized children with community-acquired Mycoplasma pneumoniae pneumonia during 2017/2018, Chengde, China[J].Medicine (Baltimore),2021,100(5):e23786.[7]Ning G,Wang X,Wu D,et al.The etiology of community-acquired pneumonia among children under 5 years of age in mainland China, 2001-2015: A systematic review[J].Hum Vaccin Immunother,2017,13(11):2742-2750.[8]中华医学会儿科学分会呼吸学组,《中华实用儿科临床杂志》编辑委员会.儿童肺炎支原体肺炎诊治专家共识(2015年版)[J].中华实用儿科临床杂志,2015,30(17):1304-1308[9]中华人民共和国国家卫生健康委员会.儿童肺炎支原体肺炎诊疗指南(2023年版)[J].中国合理用药探索,2023,20(3):16-24.[10]富琤,沙俊.肺炎支原体IgM、IgG和血清CRP、PCT水平在小儿支原体肺炎诊疗中的检测价值[J].川北医学院学报,2020,35(2):321-323,346.[11]Choo S,Kim SH,Lee E.Clinical significance of Mycoplasma pneumoniae specific IgM titer in children hospitalized with Mycoplasma pneumoniae pneumonia[J].BMC Infect Dis,2022,22(1):470.[12]Tang M,Wang D,Tong X,et al.Comparison of different detection methods for Mycoplasma pneumoniae infection in children with community-acquired pneumonia[J].BMC Pediatr,2021,21(1):90.[13]张若洋,范恩勇,王明元,等.基于PCR-化学发光的HBV/HCV/HIV并行核酸检测试剂盒的评价[J].南京医科大学学报(自然科学版),2017,37(3):335-339.[14]郭淑红.化学发光免疫法与酶联免疫吸附试验检测肺炎支原体肺炎患儿血清MP抗体结果比较[J].河南医学研究,2020,29(19):3594-3595.[15]Chen D,Wu P,Liu D,et al.Clinical role of M. pneumoniae typing antibody detected by chemiluminescent immunoassay in the diagnosis of Mycoplasma pneumoniae pneumonia in children[J].Int Immunopharmacol,2022,112:109196.[16]谢文俊.肺炎支原体抗体三种血清学检测方法的对比分析[J].中国医药科学,2019,9(11):90-92.[17]Yoon SH,Min IK,Ahn JG.Immunochromatography for the diagnosis of Mycoplasma pneumoniae infection: a systematic review and meta-analysis[J].PLoS One,2020,15(3):e0230338.[18]Tuo W,Guo X,Wu M,et al.Application value of antibody titres and RNA detection in the early prediction of Mycoplasma pneumoniae pneumonia in children: a retrospective study[J].BMC Infect Dis,2023,23(1):220.[19]Ge M,Song Q,Guo Q,et al.Diagnostic Value of Serum ELISA of Mycoplasma Pneumoniae IgM and IgG and Passive Particle Agglutination for Mycoplasma Pneumoniae Pneumonia in Children[J].Clin Lab,2021,67(1).[20]Lee WJ,Huang EY,Tsai CM,et al.Role of Serum Mycoplasma pneumoniae IgA, IgM, and IgG in the Diagnosis of Mycoplasma pneumoniae-Related Pneumonia in School-Age Children and Adolescents[J].Clin Vaccine Immunol,2017,24(1):e00471-16.

相似文献/References:

[1]李 娇,林 琳.儿童呼吸道感染者肺炎支原体及肺炎衣原体特异性抗体检测临床意义[J].医学信息,2019,32(03):96.[doi:10.3969/j.issn.1006-1959.2019.03.029]
　LI Jiao,LI Lin.Clinical Significance of Detection of Specific Antibodies Against Mycoplasma Pneumoniae and Chlamydia Pneumoniae in Children with Respiratory Tract Infection[J].Journal of Medical Information,2019,32(18):96.[doi:10.3969/j.issn.1006-1959.2019.03.029]
[2]郭昱瑄,张盛玉,王熙业,等.肺炎支原体实验室诊断的研究进展[J].医学信息,2019,32(11):26.[doi:10.3969/j.issn.1006-1959.2019.11.008]
　GUO Yu-xuan,ZHANG Sheng-Yu,WANG Xi-ye,et al.Progress in Laboratory Diagnosis of Mycoplasma Pneumoniae[J].Journal of Medical Information,2019,32(18):26.[doi:10.3969/j.issn.1006-1959.2019.11.008]
[3]石连霞.口咽部正常菌群对肺炎支原体快速培养法试剂盒的影响[J].医学信息,2019,32(11):171.[doi:10.3969/j.issn.1006-1959.2019.11.053]
　SHI Lian-xia.Effect of Normal Oropharyngeal Flora on Kit for Rapid Culture of Mycoplasma Pneumoniae[J].Journal of Medical Information,2019,32(18):171.[doi:10.3969/j.issn.1006-1959.2019.11.053]
[4]周彩虹.小儿呼吸系统感染后肺炎支原体抗体检测的意义[J].医学信息,2020,33(10):175.[doi:10.3969/j.issn.1006-1959.2020.10.054]
　ZHOU Cai-hong.Significance of Detection of Mycoplasma Pneumoniae Antibody After Respiratory Infection in Children[J].Journal of Medical Information,2020,33(18):175.[doi:10.3969/j.issn.1006-1959.2020.10.054]
[5]张同强,郑家峰,徐勇胜.暴发性肺炎支原体肺炎的临床特点及治疗[J].医学信息,2020,33(13):26.[doi:10.3969/j.issn.1006-1959.2020.13.008]
　ZHANG Tong-qiang,ZHENG Jia-feng,XU Yong-sheng.Clinical Features and Treatment of Fulminant Mycoplasma Pneumoniae Pneumonia[J].Journal of Medical Information,2020,33(18):26.[doi:10.3969/j.issn.1006-1959.2020.13.008]
[6]白经纬.微生物快速培养检测在小儿肺炎支原体感染中的诊断价值[J].医学信息,2022,35(24):151.[doi:10.3969/j.issn.1006-1959.2022.24.033]
　BAI Jing-wei.Diagnostic Value of Rapid Microbial Culture Detection in Mycoplasma Pneumoniae Infection in Children[J].Journal of Medical Information,2022,35(18):151.[doi:10.3969/j.issn.1006-1959.2022.24.033]
[7]宋 波,王玲玲,丰新倩,等.儿童肺炎支原体感染核酸与四种血清学抗体检测方法比较[J].医学信息,2024,37(18):166.[doi:10.3969/j.issn.1006-1959.2024.18.036]
　SONG Bo,WANG Ling-ling,FENG Xin-qian,et al.Comparison of Nucleic Acid and Four Serological Antibody Detection Methodsfor Mycoplasma Pneumoniae Infection in Children[J].Journal of Medical Information,2024,37(18):166.[doi:10.3969/j.issn.1006-1959.2024.18.036]

更新日期/Last Update: 1900-01-01