[1]肖超广.荧光定量反转录聚合酶链反应用于GⅠ型和GⅡ型诺如病毒的检测效能[J].医学信息,2025,38(06):127-130.[doi:10.3969/j.issn.1006-1959.2025.06.022]
　XIAO Chaoguang.Detection Efficiency of Fluorescence Quantitative Reverse Transcription Polymerase Chain Reaction for GⅠ and GⅡ Norovirus[J].Journal of Medical Information,2025,38(06):127-130.[doi:10.3969/j.issn.1006-1959.2025.06.022]
点击复制

荧光定量反转录聚合酶链反应用于GⅠ型和GⅡ型诺如病毒的检测效能()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
38卷
期数:
2025年06期
页码:
127-130
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Detection Efficiency of Fluorescence Quantitative Reverse Transcription Polymerase Chain Reaction for GⅠ and GⅡ Norovirus
文章编号:
1006-1959（2025）06-0127-04
作者:
肖超广
南昌市湾里管理局疾病预防控制中心检验股，江西 南昌 330004
Author(s):
XIAO Chaoguang
Inspection Unit, Nanchang Wanli Administration Center for Disease Control and Prevention, Nanchang 330004, Jiangxi, China
关键词:
诺如病毒; 荧光定量反转录聚合酶链反应; GⅠ型; GⅡ型; 检测效能
Keywords:
Norovirus;  Fluorescence quantitative reverse transcription polymerase chain reaction;  GⅠ type;  GⅡ type;  Detection efficiency
分类号:
R445.1
DOI:
10.3969/j.issn.1006-1959.2025.06.022
文献标志码:
A
摘要:
目的 探究荧光定量反转录聚合酶链反应（RT-PCR）对GⅠ型、GⅡ型诺如病毒（NV）的检测效能。方法 以2020年12月-2023年12月南昌市湾里管理局疾病预防控制中心收治的50例疑似NV感染患者为研究对象，采集其粪便样本，依次应用常规RT-PCR与荧光定量RT-PCR检测方式进行检验，比较两种检验方式的阳性检出率及检测效能（检测准确性、敏感度、特异度）。结果 荧光定量RT-PCR对GⅠ型、GⅡ型诺如病毒的阳性检出率高于常规RT-PCR检测（P＜0.05）。荧光定量RT-PCR对GⅠ型、GⅡ型诺如病毒的检测准确性、敏感度、特异度高于常规RT-PCR检测（P＜0.05）。结论 荧光定量RT-PCR在GⅠ型、GⅡ型诺如病毒诊断中具有较高检测效能。
Abstract:
Objective To explore the detection efficiency of fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) for GⅠ and GⅡ norovirus (NV). Methods From December 2020 to December 2023, 50 patients with suspected NV infection admitted to the Nanchang Wanli Administration Center for Disease Control and Prevention from December 2020 to December 2023 were selected as the research objects. Their fecal samples were collected and tested by conventional RT-PCR and fluorescence quantitative RT-PCR in turn. The positive detection rate and detection efficiency (detection accuracy, sensitivity, specificity) of the two test methods were compared. Results The positive detection rate of GⅠ and GⅡ norovirus by fluorescence quantitative RT-PCR was higher than that by conventional RT-PCR (P<0.05). The detection accuracy, sensitivity and specificity of GⅠ and GⅡ norovirus by fluorescence quantitative RT-PCR were higher than those by conventional RT-PCR (P<0.05). Conclusion Fluorescence quantitative RT-PCR has a high detection efficiency in the diagnosis of GⅠ and GⅡ norovirus.

参考文献/References:

[1]杨艳歌,吴占文,李涛,等. GI和GII诺如病毒ERA的可视化快速检测[J].华南理工大学学报(自然科学版),2023,51(12):140-151.[2]Dey SK,Sharif N.Evaluation of a rapid immunochromatography(IC) diagnosis kit for the detection of rotavirus and norovirus in diarrheal stool specimens in Bangladesh[J].International Journal of Infectious Diseases,2021,101(S1):180-203.[3]付瑶,刘海婷,施俊超,等.反转录酶在微小RNA实时荧光定量聚合酶链反应中的作用[J].实用检验医师杂志,2023,15(2):193-197.[4]Chhabra P,Browne H,Huynh T,et al.Single-step RT-PCRassay for dual genotyping of GI and GII norovirus strains[J].J Clin Virol,2021,134:104689.[5]Bonura F,Urone N,Bonura C,et al.Recombinant GII.P16 genotype challenges RT-PCR-based typing in region A of norovirus genome[J].J Infect,2021,83(1):69-75.[6]王娉,田卓,齐欣,等.聚乙二醇沉淀富集和预扩增反转录实时定量聚合酶链式反应高灵敏检测小浆果中低浓度甲型肝炎病毒[J].分析化学,2022,50(8):1168-1178. [7]Quee FA,de Hoog MLA,Schuurman R,et al.Community burden and transmission of acute gastroenteritis caused by norovirus and rotavirus in the Netherlands (RotaFam): a prospective household-based cohort study[J].Lancet Infect Dis,2020,20(5):598-606.[8]Han Y,Wang J,Zhang S,et al.Rapid detection of norovirus genogroup II in clinical and environmental samples using recombinase polymerase amplification[J].Anal Biochem,2020,605:113834. [9]温静,郑天驰,王希峰,等.2019年北京市大兴区诺如病毒GI引起急性胃肠炎疫情的病原学分析[J].中国卫生检验杂志,2021,31(14):1783-1786.[10]卞莲莲,王一平,孙世洋,等.基于叠氮溴化丙锭-定量反转录-聚合酶链式反应的甲型肝炎疫苗病毒滴度快速检测方法[J].中国病毒病杂志,2020,10(6):421-425. [11]祝琳,佟靖轩,姚秀林,等.2017-2019年抚顺市急性胃肠炎患者诺如病毒检测结果分析[J].中国国境卫生检疫杂志,2020,43(5):337-339. [12]赵跃丽,饶清,孙强明,等.粪便标本中诺如病毒GⅡ拷贝数定量检测方法的建立和应用[J].中国感染控制杂志,2020,19(10):864-870.[13]纪蕾,刘光涛,刘彬辉,等.一起GⅠ、GⅡ型诺如病毒混合感染疫情的病原鉴定及基因特征分析[J].中国卫生检验杂志,2020,30(13):1567-1570,1573.[14]黄晨璐,许伟,胡乾坤,等.实时荧光核酸恒温扩增试验和定量反转录-聚合酶链反应对血清乙型肝炎病毒RNA定量检测的一致性评价[J].微生物与感染,2020,15(3):158-165.[15]贾添慧,董蕾,王永杰,等.牡蛎中GⅡ型诺如病毒巢式RT-PCR检测方法的优化与评价[J].上海海洋大学学报,2021,30(2):239-246.[16]曲莉,王洪艳,李环,等.常规RT-PCR快速检测诺如病毒方法的建立[J].北华大学学报(自然科学版),2020,21(2):188-190.[17]颜伟,张静,吴杰,等. 2017-2018年滨州市病毒性腹泻流行病学特征及病原学监测分析[J].热带医学杂志,2020,20(2):264-266,278.[18]Skyum F,Chen M,Mogensen CB.Evaluation of a new fast in-house Real-Time PCR assay for detecting both Norovirus and toxigenic Clostridium difficile using fecal sample and rectal swab[J].Am J Infect Control,2022,50(1):67-71.[19]Jahne MA,Brinkman NE,Keely SP,et al.Droplet digital PCR quantification of norovirus and adenovirus in decentralized wastewater and graywater collections: Implications for onsite reuse[J].Water Res,2020,169:115213.[20]陈海丽,杨春忆,张万菊,等.2015-2016年上海地区成人门诊腹泻病例中诺如病毒GⅡ型流行特征[J].中华实验和临床病毒学杂志,2020,34(2):145-150.[21]严寒秋,王永全,崔海洋,等.应用2种RT-PCR方法检测和分析北京市市场销售牡蛎中诺如病毒基因特征[J].中华流行病学杂志,2022,43(1):92-97.[22]毛瑞,曹三成,王增国,等.673例急性腹泻患儿诺如病毒GⅠ、GⅡ型感染快速筛查结果分析[J].传染病信息,2023,36(4):351-354.[23]Kulis-Horn RK,Tiemann C.Evaluation of a laboratory-developed test for simultaneous detection of norovirus and rotavirus by real-time RT-PCR on the Panther Fusion system[J].Eur J Clin Microbiol Infect Dis,2020,39(1):103-112.

相似文献/References:

[1]潘卫兵,许 韡.诺如病毒检测技术研究[J].医学信息,2018,31(02):51.[doi:10.3969/j.issn.1006-1959.2018.02.018]
　PAN Wei-bing,XU Wei.Study on the Detection Technology of Norovirus[J].Journal of Medical Information,2018,31(06):51.[doi:10.3969/j.issn.1006-1959.2018.02.018]
[2]徐 彦,王安娜,张文增,等.一起由诺如病毒引起的感染性腹泻暴发疫情调查[J].医学信息,2018,31(15):113.[doi:10.3969/j.issn.1006-1959.2018.15.035]
　XU Yan,WANG An-na,ZHANG Wen-zeng,et al.Investigation of an Outbreak of Infectious Diarrhea Caused by Norovirus[J].Journal of Medical Information,2018,31(06):113.[doi:10.3969/j.issn.1006-1959.2018.15.035]
[3]骆玲飞,王小光,张烜昭,等.闵行区腹泻病监测人群中诺如病毒感染分析[J].医学信息,2022,35(09):1.[doi:10.3969/j.issn.1006-1959.2022.09.001]
　LUO Ling-fei,WANG Xiao-guang,ZHANG Xuan-zhao,et al.Analysis of Norovirus Infection Among Diarrhoea Surveillance Population in Minhang District[J].Journal of Medical Information,2022,35(06):1.[doi:10.3969/j.issn.1006-1959.2022.09.001]

更新日期/Last Update: 1900-01-01