[1]辛海娜,安 然.TXNDC17在乳腺癌组织中的表达及机制研究[J].医学信息,2025,38(24):60-64.[doi:10.3969/j.issn.1006-1959.2025.24.011]
　XIN Haina,AN Ran.Mechanism and Expression of TXNDC17 in Breast Cancer[J].Journal of Medical Information,2025,38(24):60-64.[doi:10.3969/j.issn.1006-1959.2025.24.011]
点击复制

TXNDC17在乳腺癌组织中的表达及机制研究()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
38卷
期数:
2025年24期
页码:
60-64
栏目:
论著
出版日期:
2025-12-15

文章信息/Info

Title:
Mechanism and Expression of TXNDC17 in Breast Cancer
文章编号:
1006-1959（2025）24-0060-05
作者:
辛海娜; 安 然
潍坊市妇幼保健院乳腺病科，山东 潍坊 261071
Author(s):
XIN Haina;  AN Ran
Department of Mastopathy, Weifang Maternal and Child Health Hospital, Weifang 261071, Shandong, China
关键词:
TXNDC17; 乳腺癌; 增殖; 侵袭
Keywords:
TXNDC17;  Breast cancer;  Proliferation;  Invasion
分类号:
R737.9
DOI:
10.3969/j.issn.1006-1959.2025.24.011
文献标志码:
A
摘要:
目的 探讨TRP14硫氧还蛋白结构域17（TXNDC17）在乳腺癌组织中的表达并研究其作用机制。方法 收集2021年6月-2023年6月潍坊市妇幼保健院乳腺病科术后乳腺癌组织及癌旁组织各60例，利用实时荧光定量聚合酶链反应（RT-PCR）法检测TXNDC17在乳腺癌组织和癌旁组织中的表达，RT-PCR检测乳腺癌细胞系MCF7、MDAMB231及人正常乳腺细胞系MCF10A中TXNDC17的表达，选取高表达TXNDC17的细胞系MDAMB231为对象，将其分为实验组（转染TXNDC17 inhibitor）、阴性对照组（转染inhibitor NC）、空白组（自然生长细胞），培养后采用RT-PCR及Western blot检测TXNDC17的表达，CCK-8实验检测各组细胞增殖能力，Transwell实验检测各组细胞侵袭能力。结果 在乳腺癌组织中TXNDC17的表达水平高于癌旁组织（P＜0.05），且TXNDC17表达量与乳腺癌的组织学分级、Ki-67表达率及淋巴结转移率呈正相关（P=0.031、0.022、0.035）。TXNDC17在乳腺癌细胞系MCF7、MDAMB231中的表达水平高于MCF10A细胞系（P＜0.05）。转染TXNDC17抑制剂后，实验组TXNDC17 mRNA及其蛋白的表达水平低于阴性对照组和空白组（P＜0.05），实验组细胞的增殖、侵袭能力受到抑制（P＜0.05）。结论 TXNDC17在乳腺癌组织中高表达，其表达水平与组织学分级、Ki-67表达率和淋巴结转移呈正相关。抑制TXNDC17表达水平后，乳腺癌细胞的增殖、侵袭能力明显下降。
Abstract:
Objective To investigate the expression of TXNDC17 in breast cancer tissues and its mechanism. Methods From June 2021 to June 2023, 60 cases of breast cancer tissues and adjacent tissues were collected from the Department of Mastopathy, Weifang Maternal and Child Health Hospital. The expression of TXNDC17 in breast cancer tissues and adjacent tissues was detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). The expression of TXNDC17 in breast cancer cell lines MCF7, MDAMB231 and human normal breast cell line MCF10A was detected by RT-PCR. The cell line MDAMB231 with high expression of TXNDC17 was selected as the object. They were divided into experimental group (transfected with TXNDC17 inhibitor), negative control group (transfected with inhibitor NC) and blank group (natural growth cells). After culture, the expression of TXNDC17 was detected by RT-PCR and Western blot. CCK-8 assay was used to detect the cell proliferation ability of each group, and Transwell assay was used to detect the cell invasion ability of each group. Results The expression level of TXNDC17 in breast cancer tissues was higher than that in adjacent tissues (P<0.05), and the expression of TXNDC17 was positively correlated with the histological grade, Ki-67 expression rate and lymph node metastasis rate of breast cancer (P=0.031, 0.022, 0.035). The expression level of TXNDC17 in breast cancer cell lines MCF7 and MDAMB231 was higher than that in MCF10A cell line (P<0.05). After transfection with TXNDC17 inhibitor, the expression levels of TXNDC17 mRNA and protein in the experimental group were lower than those in the negative control group and the blank group (P<0.05), and the proliferation and invasion ability of the experimental group were inhibited (P<0.05). Conclusion TXNDC17 is highly expressed in breast cancer tissues, and its expression level is positively correlated with histological grade, Ki-67 expression rate and lymph node metastasis. After inhibiting the expression level of TXNDC17, the proliferation and invasion ability of breast cancer cells decreased significantly.

参考文献/References:

[1]Jeong W,Yoon HW,Lee SR,et al.Identification and characterization of TRP14,a thioredoxin-related protein of 14 kDa. New insights into the specificity of thioredoxin function[J].J Biol Chem,2004,279(5):3142-3150.[2]Meinhold DW,Felitsky DJ,Dyson HJ,et al.Transient On- and Off-Pathway Protein Folding Intermediate States Characterized with NMR Relaxation Dispersion[J].J Phys Chem B,2022,126(46):9539-9548.[3]Zhang SF,Wang XY,Fu ZQ,et al.TXNDC17 promotes paclitaxel resistance via inducing autophagy in ovarian cancer.Autophagy[J].Autophagy,2015,11(2):225-238.[4]彭巧华,俞燕,程晓东,等.TXNDC17表达与子宫内膜浆液性癌患者临床病理特征及预后的关系研究[J].中华医学杂志,2022,102(32):2506-2512.[5]Montero-Calle A,Garranzo-Asensio M,Poves C,et al.In-Depth Proteomic Analysis of Paraffin-Embedded Tissue Samples from Colorectal Cancer Patients Revealed TXNDC17 and SLC8A1 as Key Proteins Associated with the Disease[J].J Proteome Res,2024,23(11):4802-4820.[6]陈彩萍,钱燕芳.WGCNA联合LASSO-COX分析筛选并构建乳腺癌患者5基因预后预测模型[J].全科医学临床与教育,2023,21(4):336-339,封3.[7]Du XJ,Yang XR,Wang QC,et al.Identification and validation of a five-gene prognostic signature based on bioinformatics analyses in breast cancer[J].Heliyon,2023,9(2):e13185.[8]Kim J,Harper A,McCormack V,et al.Global patterns and trends in breast cancer incidence and mortality across 185 countries[J].Nat Med,2025,31(4):1154-1162.[9]Storme GA.Breast Cancer: Impact of New Treatments?[J].Cancers (Basel),2023,15(8):2205.[10]Bae SY,Kamalanathan KJ,Galeano-Garces C,et al.Dissemination of Circulating Tumor Cells in Breast and Prostate Cancer: Implications for Early Detection[J].Endocrinology,2024,165(4):bqae022.[11]Debnath P,Huirem RS,Dutta P,et al.Epithelial-mesenchymal transition and its transcription factors[J].Biosci Rep,2022,42(1):BSR20211754.[12]Yang Y,Neo SY,Chen Z,et al.Thioredoxin activity confers resistance against oxidative stress in tumor-infiltrating NK cells[J].J Clin Invest,2020,130(10):5508-5522.[13]闫红江,任红欣,高伟年,等.TRP14促进非小细胞肺癌细胞增殖移动的作用机制研究[J].循证医学,2021,21(5):294-300.[14]Shang W,Xie Z,Lu F,et al.Increased Thioredoxin-1 Expression Promotes Cancer Progression and Predicts Poor Prognosis in Patients with Gastric Cancer[J].Oxid Med Cell Longev,2019,2019:9291683.[15]Martí-Andrés P,Finamor I,Torres-Cuevas I,et al.TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation[J].EMBO J,2024,43(13):2789-2812.[16]Brandl N,Seitz R,Sendtner N,et al.Living on the Edge: ROS Homeostasis in Cancer Cells and Its Potential as a Therapeutic Target.Antioxidants (Basel)[J].Antioxidants (Basel),2025,14(8):1002.[17]Jeong W,Jung Y,Kim H,et al.Thioredoxin-related protein 14,a new member of the thioredoxin family with disulfide reductase activity: implication in the redox regulation of TNF-alpha signaling[J].Free Radic Biol Med,2009,47(9):1294-1303.[18]Young TM,Reyes C,Pasnikowski E,et al.Autophagy protects tumors from T cell-mediated cytotoxicity via inhibition of TNFα-induced apoptosis.Sci Immunol[J].Sci Immunol,2020,5(54):eabb9561.[19]Tyciakova S,Valova V,Svitkova B,et al.Overexpression of TNFα induces senescence,autophagy and mitochondrial dysfunctions in melanoma cells[J].BMC Cancer,2021,21(1):507.[20]Guenter J,Abadi S,Lim H,et al.Evaluating genomic biomarkers associated with resistance or sensitivity to chemotherapy in patients with advanced breast and colorectal cancer[J].J Oncol Pharm Pract,2021,27(6):1371-1381.[21]Zeng C,Li Z,Wei Z,et al.Mechanism of Drug Resistance to First-Line Chemotherapeutics Mediated by TXNDC17 in Neuroblastomas[J].Cancer Rep (Hoboken),2024,(10):e70033.[22]Guenter J,Abadi S,Lim H,et al.Evaluating genomic biomarkers associated with resistance or sensitivity to chemotherapy in patients with advanced breast and colorectal cancer[J].J Oncol Pharm Pract,2021,27(6):1371-1381.

相似文献/References:

[1]董 云.SPECT/CT 双时相99mTc- MIBI断层显像 在乳腺癌诊断中的应用[J].医学信息,2018,31(05):147.[doi:10.3969/j.issn.1006-1959.2018.05.053]
　DONG Yun.Application of SPECT/CT Dual-phase 99mTc-MIBI Tomography in the Diagnosis of Breast Cancer[J].Journal of Medical Information,2018,31(24):147.[doi:10.3969/j.issn.1006-1959.2018.05.053]
[2]张 虎,张 涛,徐 芳,等.MRI在乳腺癌诊治中的研究进展[J].医学信息,2022,35(10):62.[doi:10.3969/j.issn.1006-1959.2022.10.015]
　ZHANG Hu,ZHANG Tao,XU Fang,et al.Research Progress of MRI in Diagnosis and Treatment of Breast Cancer[J].Journal of Medical Information,2022,35(24):62.[doi:10.3969/j.issn.1006-1959.2022.10.015]
[3]李建辉,祝旭龙.乳腺癌的治疗管理——把好生活质量关[J].医学信息,2018,31(13):1.[doi:10.3969/j.issn.1006-1959.2018.13.001]
[4]杨玉峰,李 强,曲国红,等.乳腺癌术前巴德枪穿刺检测ER、PR的临床研究[J].医学信息,2018,31(13):163.[doi:10.3969/j.issn.1006-1959.2018.13.050]
　YANG Yu-feng,LI Qiang,QU Guo-hong,et al.Clinical Study on Preoperative ER and PR Detection of Bard Gun Puncture in Breast Cancer[J].Journal of Medical Information,2018,31(24):163.[doi:10.3969/j.issn.1006-1959.2018.13.050]
[5]刘雪静,路 红,徐熠琳,等.乳腺影像与容积导航技术初步应用的临床分析[J].医学信息,2022,35(11):161.[doi:10.3969/j.issn.1006-1959.2022.11.043]
　LIU Xue-jing,LU Hong,XU Yi-lin,et al.Clinical Analysis of the Preliminary Application of Breast Imaging and Volume Navigation Technology[J].Journal of Medical Information,2022,35(24):161.[doi:10.3969/j.issn.1006-1959.2022.11.043]
[6]刘在波,修文娟.背阔肌皮瓣联合假体在乳腺癌改良根治术后乳房重建中的应用[J].医学信息,2019,32(02):127.[doi:10.3969/j.issn.1006-1959.2019.02.036]
　LIU Zai-bo,XIU Wen-juan.Application of Latissimus Dorsi Flap Combined with Prosthesis in Breast Reconstruction after Modified Radical Mastectomy for Breast Cancer[J].Journal of Medical Information,2019,32(24):127.[doi:10.3969/j.issn.1006-1959.2019.02.036]
[7]孟庆国,王佳雪,王斯佳.马来酸吡咯替尼对乳腺癌术后患者心脏功能的影响[J].医学信息,2019,32(02):152.[doi:10.3969/j.issn.1006-1959.2019.02.045]
　MENG Qing-guo,WANG Jia-xue,WANG Si-jia.Effect of Pyrrolidine Maleate on Cardiac Function in Patients after Breast Cancer Operation[J].Journal of Medical Information,2019,32(24):152.[doi:10.3969/j.issn.1006-1959.2019.02.045]
[8]刘 曼,厉玛倩倩,徐润润,等.延续性护理对乳腺癌术后化疗患者生活质量与 健康知识水平的影响[J].医学信息,2018,31(16):178.[doi:10.3969/j.issn.1006-1959.2018.16.057]
　LIU Man,LIMA Qian-qian,XU Run-run,et al.Effect of Continuous Nursing on Quality of Life and Health Knowledge of Postoperative Chemotherapy Patients with Breast Cancer[J].Journal of Medical Information,2018,31(24):178.[doi:10.3969/j.issn.1006-1959.2018.16.057]
[9]满枋霖,葛俊宏,林晓月,等.p21活化激酶在乳腺癌中的作用研究[J].医学信息,2019,32(06):35.[doi:10.3969/j.issn.1006-1959.2019.06.013]
　MAN Fang-lin,GE Jun-hong,LIN Xiao-yue,et al.The Role of p21 Activated Kinase in Breast Cancer[J].Journal of Medical Information,2019,32(24):35.[doi:10.3969/j.issn.1006-1959.2019.06.013]
[10]张天竺,宁 勇.长链非编码RNA调控乳腺癌发生发展的研究进展[J].医学信息,2019,32(12):40.[doi:10.3969/j.issn.1006-1959.2019.12.014]
　ZHANG Tian-zhu,NING Yong.Advances in Research on Long-chain Non-coding RNA Regulation of Breast Cancer Development[J].Journal of Medical Information,2019,32(24):40.[doi:10.3969/j.issn.1006-1959.2019.12.014]

更新日期/Last Update: 1900-01-01