[1]马 晔,陈跃宇.不同碘浓度对大鼠甲状腺摄碘功能的影响[J].医学信息,2018,31(23):83-85.[doi:10.3969/j.issn.1006-1959.2018.23.023]
　MA Ye,CHEN Yue-yu.Effects of Different Iodine Concentrations on Thyroid Iodine Uptake in Rats[J].Journal of Medical Information,2018,31(23):83-85.[doi:10.3969/j.issn.1006-1959.2018.23.023]
点击复制

不同碘浓度对大鼠甲状腺摄碘功能的影响()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年23期
页码:
83-85
栏目:
论著
出版日期:
2018-12-01

文章信息/Info

Title:
Effects of Different Iodine Concentrations on Thyroid Iodine Uptake in Rats
文章编号:
1006-1959（2018）23-0083-03
作者:
马 晔; 陈跃宇
上海市嘉定区中心医院普通外科，上海 201899
Author(s):
MA Ye; CHEN Yue-yu
Department of Surgery,Jiading Central Hospital,Shanghai 201899,China
关键词:
碘; 甲状腺; 钠碘转运体; 促甲状腺激素受体; 甲状腺过氧化物酶抗体
Keywords:
Iodine; Thyroid; Sodium iodide transporter; Thyrotropin receptor; Thyroid peroxidase antibody
分类号:
R581
DOI:
10.3969/j.issn.1006-1959.2018.23.023
文献标志码:
A
摘要:
观察不同碘摄入量对大鼠促甲状腺激素受体（TSHR）mRNA、钠碘转运体（NIS）mRNA以及血清过氧化物酶抗体（TPOAb）表达水平的影响。方法 SD大鼠30只，随机分成低碘组（LI）、适碘组（NI）和高碘组（HI），每组10只。于实验第8、16周分批处死。实时荧光定量聚合酶链式反应法测定TSHR mRNA及NIS mRNA表达水平。酶联免疫分析法观察血清TPOAb浓度。结果 实验8周，低碘组和高碘组TSHR mRNA及NIS mRNA表达水平均低于适碘组，差异有统计学意义（P＜0.05）；且血清TPOAb浓度高于适碘组，但差异无统计学差异（P＞0.05）；实验16周，高碘组和低碘组的TSHR mRNA和NISmRNA表达水平均明显低于适碘组，差异有统计学意义（P＜0.05）；血清TPOAb浓度高于适碘组，差异有统计学意义（P＜0.05）。结论 包括碘过量和碘缺乏在内的长期碘营养障碍均可降低NIS及TSHR的正常表达，同时提高甲状腺自身抗体的浓度，影响甲状腺摄碘功能。
Abstract:
Objective To investigate the effects of different iodine intake on the expression of (TSHR) mRNA, sodium iodide transporter (NIS) mRNA and peroxidase antibody (TPOAb) in rat thyroid stimulating hormone receptor.Methods 30 SD rats were randomly divided into two groups: low iodine group (LI), normal iodine group (NI) and high iodine group (HI),) 10 rats in each group. The rats were executed in batches at the 8th ,16th week.The expression levels of TSHR mRNA and NIS mRNA were measured by real-time fluorescence quantitative polymerase chain reaction. The concentration of serum TPOAb was observed by enzyme linked immunosorbent assay.Results At the 8th week of the experiment, the expression of TSHR mRNA and NIS mRNA in iodine deficiency group and high iodine group were lower than that in normal iodine group,the difference was statistically significant (P＜0.05).The concentration of serum TPOAb was higher than that of iodine group, but there was no significant difference (P＞0.05).At the 16th week of experiment, the expression levels of TSHR mRNA and NISmRNA in high iodine group and low iodine group were significantly lower than those in normal iodine group,the difference was statistically significant (P＜0.05).The concentration of serum TPOAb was higher than that of iodine group,the difference was statistically significant (P＜0.05).Conclusion Chronic iodine nutritional disorder,including iodine excess and iodine deficiency, can decrease the normal expression of NIS and TSHR, increase the concentration of thyroid autoantibodies and affect the iodine uptake function of thyroid.

参考文献/References:

[1]Ravera S,Reyna-Neyra A,Ferrandino G,et al.The Sodium/Iodide Symporter (NIS): Molecular Physiology and Preclinical and Clinical Applications[J].Annu Rev Physiol,2017,10(79):261-289.
[2]田文霞,孙文广.碘与甲状腺疾病的相关研究[J].医学综述,2017,23(24):4868-4872.
[3]陈雪梅,刘燚,范源,等.碘过量与桥本氏甲状腺炎之间关系的研究进展[J].昆明医科大学学报,2014,35(12):169-174.
[4]Chen XY,Lin CH,Yang LH,et al.The Effect on Sodium/Iodide Symporter and Pendrin in Thyroid Colloid Retention Developed byExcess Iodide Intake[J].Biol Trace Elem Res,2016 172(1):193-200.
[5]Arriagada AA,Albornoz E,Opazo MC,et al.Excess iodide induces an acute inhibition of the sodium/iodide symporter in thyroid male rat cells by increasing reactive oxygen species[J].Endocrinology,2015,156(4):1540-1551.
[6]Kim S,Chung JK,Min HS,et al.Expression patterns of glucose transporter-1 gene and thyroid specific genes in human papillarythyroid carcinoma[J].Nucl Med Mol Imaging,2014,48(2):91-97.
[7]Serrano-Nascimento C,Nicola JP,Teixeira Sda S，et al.Excess iodide downregulates Na(+)/I(-) symporter gene transcription through activation of PI3K/Akt pathway[J].Mol Cell Endocrinol,2016 5(426):73-90.
[8]王绵.碘营养状态与甲状腺疾病[J].临床荟萃,2016,31(3):272-276.
[9]苏俊平,张博,陈云霞,等.不同碘摄入量对自身免疫性甲状腺炎免疫机制的影响[J].临床荟萃,2013,28(8):889-891.
[10]Zimmermann MB,Boelaert K.Iodine deficiency and thyroid disoeders[J].Lancet Diabetes Emdocrinol,2015,3(4):286-295.
[11]Koukkou EG,Roupas ND,Markou KB.Effect of excess iodine intake on thyroid on human health[J].Minerva Med,2017,108(2):136-146.

相似文献/References:

[1]肖 倩.护理干预对甲状腺手术患者疼痛及负性情绪的影响分析[J].医学信息,2018,31(20):180.[doi:10.3969/j.issn.1006-1959.2018.20.057]
　XIAO Qian.Analysis of the Effect of Nursing Intervention on Pain and Negative Emotion in Patients with Thyroid Surgery[J].Journal of Medical Information,2018,31(23):180.[doi:10.3969/j.issn.1006-1959.2018.20.057]
[2]孙 阳.甲状腺结节钙化在超声诊断甲状腺癌中的价值[J].医学信息,2019,32(24):173.[doi:10.3969/j.issn.1006-1959.2019.24.064]
　SUN Yang.The Value of Thyroid Nodule Calcification in Ultrasound Diagnosis of Thyroid Cancer[J].Journal of Medical Information,2019,32(23):173.[doi:10.3969/j.issn.1006-1959.2019.24.064]
[3]孙洪洋,刘文波,姜 阳,等.二维超声与彩色多普勒超声诊断甲状腺良恶性结节的临床应用价值[J].医学信息,2022,35(03):178.[doi:10.3969/j.issn.1006-1959.2022.03.048]
　SUN Hong-yang,LIU Wen-bo,JIANG Yang,et al.The Clinical Application Value of Two-dimensional Ultrasound and Color Doppler Ultrasoundin the Diagnosis of Benign and Malignant Thyroid Nodules[J].Journal of Medical Information,2022,35(23):178.[doi:10.3969/j.issn.1006-1959.2022.03.048]

更新日期/Last Update: 2018-12-17