[1]李 新,田 蜜,王 程.慢病毒sh-SIRT1载体对乳腺癌细胞增殖、迁移和侵袭的影响[J].医学信息,2021,34(06):1-5.[doi:10.3969/j.issn.1006-1959.2021.06.001]
 LI Xin,TIAN Mi,WANG Cheng.Effects of Lentiviral sh-SIRT1 Vector on the Proliferation,Migration and Invasion of Breast Cancer Cells[J].Medical Information,2021,34(06):1-5.[doi:10.3969/j.issn.1006-1959.2021.06.001]
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慢病毒sh-SIRT1载体对乳腺癌细胞增殖、迁移和侵袭的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年06期
页码:
1-5
栏目:
出版日期:
2021-03-15

文章信息/Info

Title:
Effects of Lentiviral sh-SIRT1 Vector on the Proliferation,Migration and Invasion of Breast Cancer Cells
文章编号:
1006-1959(2021)06-0001-05
作者:
李 新田 蜜王 程
(1.荆门市第二人民医院甲乳外科,湖北 荆门 448000; 2.荆门市公安局法医鉴定中心,湖北 荆门 448000)
Author(s):
LI XinTIAN MiWANG Cheng
(1.Department of Thoracic and Breast Surgery,Jingmen Second People’s Hospital,Jingmen 448000,Hubei,China; 2.Forensic Appraisal Center of Jingmen Public Security Bureau,Jingmen 448000,Hubei,China)
关键词:
shRNA慢病毒载体SIRT1乳腺癌增殖
Keywords:
shRNA lentiviral vectorSIRT1Breast cancerProliferation
分类号:
R737.9
DOI:
10.3969/j.issn.1006-1959.2021.06.001
文献标志码:
A
摘要:
目的 探讨慢病毒sh-SIRT1载体对乳腺癌细胞增殖、迁移和侵袭的影响。方法 收集2017年1月~2019年12月荆门市第二人民医院确诊的80例乳腺癌患者的癌组织及癌旁正常组织,采用qPCR方法检测SIRT1基因的表达水平,qPCR和免疫印迹方法检测慢病毒sh-SIRT1载体的有效性,MTT法检测乳腺癌细胞活性,Transwell法检测乳腺癌细胞的侵袭和迁移,Western Blot检测乳腺癌细胞EMT相关蛋白的表达。结果 SIRT1在乳腺癌中呈高度表达,癌旁组织中呈低表达;与人乳腺上皮细胞(MCF-10A)相比,乳腺癌细胞系(MDA-MB-231、SK-BR-3)中SIRT1的基因表达增加。不同年龄、吸烟行为、酗酒行为间SIRT1表达比较,差异无统计学意义(P>0.05);不同T分期、N分期、M分期间SIRT1表达比较,差异有统计学意义(P<0.05)。MTT法检测显示,在MDA-MB-231细胞和SK-BR-3细胞中sh-SIRT1可抑制乳腺癌细胞的增殖;Transwell法检测显示,在MDA-MB-231细胞中sh-SIRT1可抑制乳腺癌细胞的迁移和侵袭;Western Blot检测显示,sh-SIRT1可抑制α-catenin、PTEN和E-cadherin表达下调,促进N-cadherin、β-catenin和Vimentin表达上调。结论 SIRT1在乳腺癌细胞中高表达,慢病毒sh-SIRT1载体可抑制乳腺癌细胞的增殖、迁移和侵袭。
Abstract:
Objective To investigate the effect of lentiviral sh-SIRT1 vector on the proliferation, migration and invasion of breast cancer cells.Methods Collected the cancer tissues and adjacent normal tissues of 80 breast cancer patients diagnosed in Jingmen Second People’s Hospital from January 2017 to December 2019. qPCR method was used to detect the expression level of SIRT1 gene, qPCR and western blot methods were used to detect the effectiveness of lentiviral sh-SIRT1 vector, MTT method was used to detect breast cancer cell viability, the Transwell method was used to detect breast cancer cell invasion and migration, and Western Blot was used to detect breast cancer. Expression of EMT-related proteins in cells.Results SIRT1 was highly expressed in breast cancer and low in adjacent tissues; compared with human breast epithelial cells (MCF-10A),increased expression of SIRT1 gene in breast cancer cell lines (MDA-MB-231, SK-BR-3);There was no statistically significant difference in the expression of SIRT1 among different ages, smoking behaviors, and alcohol abuse behaviors (P>0.05).Comparison of SIRT1 expression during different T stage, N stage and M stage, the difference was statistically significant (P<0.05);MqTT assay found that sh-SIRT1 could inhibit the proliferation of breast cancer cells in MDA-MB-231 cells and SK-BR-3 cells;Transwell assay found that sh-SIRT1 could inhibit the migration and invasion of breast cancer cells in MDA-MB-231 cells.Western Blot detection found that sh-SIRT1 could inhibit the down-regulation of α-catenin, PTEN and E-cadherin expression, and promote the up-regulation of N-cadherin, β-catenin and Vimentin expression.Conclusion SIRT1 is highly expressed in breast cancer cells. The lentiviral sh-SIRT1 vector can inhibit the proliferation, migration and invasion of breast cancer cells.

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更新日期/Last Update: 1900-01-01