[1]刘 佳,陶庆春,李公民,等.清开灵、莲必治分别联合不同抗生素对ICU耐碳青霉烯鲍曼不动杆菌的体外抑菌效果[J].医学信息,2021,34(12):162-165.[doi:10.3969/j.issn.1006-1959.2021.12.044]
　LIU Jia,TAO Qing-chun,LI Gong-min,et al.Effect of Qingkailing and Lianbizhi Combined with Different Antibiotics on ICU Carbapenem-resistant Acinetobacter Baumannii[J].Medical Information,2021,34(12):162-165.[doi:10.3969/j.issn.1006-1959.2021.12.044]
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清开灵、莲必治分别联合不同抗生素对ICU耐碳青霉烯鲍曼不动杆菌的体外抑菌效果()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年12期
页码:
162-165
栏目:
药物与临床
出版日期:
2021-06-15

文章信息/Info

Title:
Effect of Qingkailing and Lianbizhi Combined with Different Antibiotics on ICU Carbapenem-resistant Acinetobacter Baumannii
文章编号:
1006-1959（2021）12-0162-04
作者:
刘 佳; 陶庆春; 李公民; 等
（1.北京市房山区中医医院检验科，北京 102400； 2北京中医药大学第三附属医院检验科，北京 100029； 3.北京燕化医院检验科，北京 102500； 4.北京良乡医院检验科，北京 102401； 5.北京市房山区第一医院检验科，北京 102400）
Author(s):
LIU Jia; TAO Qing-chun; LI Gong-min; et al.
(1.Department of Laboratory Medicine,Fangshan Traditional Chinese Medicine Hospital of Beijing,Beijing 102400,China; 2.Department of Laboratory Medicine,the Third Affiliated Hospital of Beijing University of Traditional Chinese Medicine, Beijing 100029,China; 3.Department of Laboratory Medicine,Beijing Yanhua Hospital,Beijing 102500,China; 4.Department of Laboratory Medicine,Beijing Liangxiang Hospital,Beijing 102401,China; 5.Department of Laboratory Medicine,the First Hospital of Fangshan District,Beijing,Beijing 102400,China)
关键词:
清开灵; 莲必治; 耐碳青霉烯鲍曼不动杆菌
Keywords:
Qingkailing; Lianbizhi; Carbapenem-resistant Acinetobacter baumannii
分类号:
R969
DOI:
10.3969/j.issn.1006-1959.2021.12.044
文献标志码:
A
摘要:
目的 研究清开灵、莲必治注射液分别联合头孢他啶、头孢哌酮钠舒巴坦钠、哌拉西林钠他唑巴坦及亚胺培南西司他丁钠对耐碳青霉烯鲍曼不动杆菌的抑菌效果。方法 采用微量肉汤棋盘法测定不同浓度组合清开灵、莲必治与头孢他啶、头孢哌酮钠舒巴坦钠、哌拉西林钠他唑巴坦及亚胺培南西司他丁钠对ICU收集分离的30株耐碳青霉烯鲍曼不动杆菌的最低抑菌浓度，计算分级抑菌浓度（FIC）指数判定中西药联合抑菌作用。结果 莲必治单用有1株抑菌浓度MIC为1∶2，其余29株MIC均为1∶1；清开灵单用MIC值范围为1∶4~1∶2。联用头孢哌酮钠舒巴坦钠的MIC50和MIC90分别是32 μg/ml、64 μg/ml，另外三种抗生素的MIC50、MIC90均为64 μg/ml；清开灵联合头孢哌酮钠舒巴坦钠的MIC50降低最明显。清开灵1∶8+头孢他啶64 μg/ml菌株数量分布最多，占80.00%；清开灵1∶16+头孢哌酮钠舒巴坦钠64 μg/ml、清开灵1∶8+头孢哌酮钠舒巴坦钠64 μg/ml菌株数量相同，占23.33%；清开灵1∶8+哌拉西林钠他唑巴坦64 μg/ml菌株数量最多，占46.67%；清开灵1∶8+亚胺培南西司他丁钠64 μg/ml菌株数量最多，占53.33%。清开灵分别联合四种抗生素的FIC协同作用范围为13.33%~40.00%，相加作用范围为46.66%~80.00%，无关作用范围为6.67%~26.67%，拮抗作用均为0。结论 莲必治注射液单用未能显示较好的体外抑菌作用，而清开灵注射液联合头孢哌酮钠舒巴坦钠、哌拉西林钠他唑巴坦相加和协同作用明显，对耐碳青霉烯鲍曼不动杆菌体外抑菌效果较好。
Abstract:
Objective To study the effects of Qingkailing and Lianbizhi injection combined with ceftazidime, cefoperazone sodium and sulbactam sodium, piperacillin sodium, tazobactam and imipenem and cilastatin sodium on carbapenem-resistant Acinetobacter baumannii Antibacterial effect.Methods The trace broth chessboard method was used to determine the different concentrations of Qingkailing, Lianbizhi and ceftazidime, cefoperazone sodium and sulbactam sodium, piperacillin sodium tazobactam and imipenem and cilastatin sodium collected and separated in the ICU. The minimum inhibitory concentration of a strain of carbapenem-resistant Acinetobacter baumannii,calculate the graded inhibitory concentration (FIC) index to determine the combined antibacterial effect of Chinese and Western medicine.Results Lianbizhi alone had a bacteriostatic concentration of 1:2.The MIC of the remaining 29 strains were all 1:1; the MIC value range of Qingkailing alone was 1:4~1:2.The MIC50 and MIC90 of the combined use of cefoperazone sodium and sulbactam sodium are 32 μg/ml and 64 μg/ml, respectively, and the MIC50 and MIC90 of the other three antibiotics were both 64 μg/ml;The MIC50 of Qingkailing combined with cefoperazone sodium and sulbactam sodium decreased most significantly. Qingkailing 1:8+ceftazidime 64 μg/ml strains were the most distributed, accounting for 80.00%;Qingkailing 1:16+cefoperazone sodium and sulbactam sodium 64 μg/ml, Qingkailing 1:8+cefoperazone sodium and sulbactam sodium 64 μg/ml had the same number of strains, accounting for 23.33%;Qingkailing 1:8+piperacillin sodium and tazobactam 64 μg/ml strains were the most numerous, accounting for 46.67%;Qingkailing 1:8 + imipenem and cilastatin sodium 64 μg/ml had the largest number of strains, accounting for 53.33%.The FIC synergistic range of Qingkailing combined with four antibiotics was 13.33%~40.00%,The additive effect range was 46.66%~80.00%, the irrelevant effect range was 6.67%~26.67%,the antagonism was zero.Conclusion Lianbizhi injection alone fails to show good antibacterial effect in vitro, while Qingkailing injection combined with cefoperazone sodium and sulbactam sodium, piperacillin sodium and tazobactam have an obvious additive and synergistic effect, and has obvious synergistic effects on carbon tolerance. Penicillium Acinetobacter baumannii has a better antibacterial effect in vitro.

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更新日期/Last Update: 1900-01-01