[1]胡铁汉,李 程,司原成,等.毫针针刺对SD大鼠腧穴筋膜组织CSK、ATP和PCNA影响[J].医学信息,2022,35(03):168-171.[doi:10.3969/j.issn.1006-1959.2022.03.045]
　HU Tie-han,LI Chen,SI Yuan-cheng,et al.The Effect of CSK, ATP and PCNA in the Fascia Tissues of Acupoints on SD Ratsby Filiform Needle Acupuncture[J].Medical Information,2022,35(03):168-171.[doi:10.3969/j.issn.1006-1959.2022.03.045]
点击复制

毫针针刺对SD大鼠腧穴筋膜组织CSK、ATP和PCNA影响()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年03期
页码:
168-171
栏目:
中医中药
出版日期:
2022-02-01

文章信息/Info

Title:
The Effect of CSK, ATP and PCNA in the Fascia Tissues of Acupoints on SD Ratsby Filiform Needle Acupuncture
文章编号:
1006-1959（2022）03-0168-03
作者:
胡铁汉1; 2; 李 程1; 2; 司原成1; 陈 波1; 张 婷1; 赖 清1
1.贵州中医药大学针灸推拿学院，贵州 贵阳 550025；2.贵州省第二人民医院中医科，贵州 贵阳 550004
Author(s):
HU Tie-han1; 2; LI Chen1; 2; SI Yuan-cheng1; CHEN Bo1; ZHANG Ting1; LAI Ting1
1.College of Acupuncture and Tuina,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;2.Department of Traditional Chinese Medicine,Guizhou Second People’s Hospital,Guiyang 550004,Guizhou,China
关键词:
针刺; 力学信号传导; CSK; ATP; PCNA
Keywords:
Acupuncture; Mechanical signal transduction; CSK; ATP; PCNA
分类号:
R245
DOI:
10.3969/j.issn.1006-1959.2022.03.045
文献标志码:
A
摘要:
目的 研究毫针针刺对腧穴筋膜组织细胞骨架（CSK）的调节作用及其对三磷酸腺苷（ATP）含量、增殖细胞核抗原（PCNA）表达的影响，为针刺作用原理提供新的实验证据。方法 将16只SPF级SD大鼠，随机均分为空白组（n=8）和针刺组（n=8），针刺组针刺中脘穴，捻转行针（60±3）次/min ，共5 min，留针10 min，共操作3轮后出针，1次/d，连续3 d。术毕剪取以大鼠中脘穴的穴点为圆心、直径约0.5 cm区域的筋膜组织块。采用免疫组织化学法检测CSK微丝（actin）、中间丝（vimentin）、微管（tubulin）和PCNA的差异变化，应用ELISA法检测组织中ATP的含量差异。结果 针刺组的CSK微丝、微管表达的平均光密度（Mean density，MD）均低于空白组（P＜0.01）；两组中间丝的表达比较，差异无统计学意义（P＞0.05）；针刺组腧穴组织ATP含量及PCNA阳性细胞计数率均高于空白组（P＜0.01）。结论 毫针针刺可能通过细胞骨架通路，增强腧穴组织能量代谢和细胞活力，进而激发针刺治疗作用，是现代针灸医学研究的机理之一。
Abstract:
Objective To study the regulatory effect of filiform needle acupuncture on the cytoskeleton (CSK) of acupoints’ fascia tissue and its effect on ATP content and PCNA expression, so as to provide new experimental evidence for the mechanism of acupuncture.Methods A total of 16 SPF SD rats were randomly divided into blank group (n=8) and acupuncture group (n=8). The acupuncture group was treated with acupuncture at Zhongwan acupoint, twisting the needle (60±3) times/min for 5 min, retaining the needle for 10 min, and the needle was removed after 3 rounds of operation, once daily for 3 days. At the end of the operation, the fascia tissue blocks with the central point of Zhongwan point in rats as the center and the diameter of about 0.5 cm were cut. Immunohistochemistry was used to detect the difference of actin, vimentin, tubulin and PCNA in CSK, and ELISA was used to detect the difference of ATP content in tissues.Results The mean density (MD) of CSK filaments and microtubules in acupuncture group was lower than that in blank group (P<0.01); there was no significant difference in the expression of interfilament between the two groups (P>0.05). ATP content and PCNA positive cell count rate in acupuncture group were higher than those in blank group (P<0.01).Conclusion Filiform needle acupuncture may enhance the energy metabolism and cell vitality of acupoints through the cytoskeleton pathway, thereby stimulating the therapeutic effect of acupuncture, which is one of the mechanisms of modern acupuncture medical research.

参考文献/References:

[1]郭义.实验针灸学(全国高等中医药院校规划教材第十一版,新世纪第五版)[M].北京:中国中医药出版社,2021:51-75.[2]余曙光,徐斌.实验针灸学(第2版)[M].北京:人民卫生出版社,2020:7-25,67-72.[3]李程,陈波,胡铁汉,等.毫针针刺对腧穴筋膜结缔组织细胞整合素β1、β3亚基和PCNA表达的影响[J].中国老年学杂志,2015,35(16):4442-4444.[4]杨榕,缪辉宇,黄志慧.针刺联合拔罐运动疗法治疗膝骨关节炎的临床观察[J].上海针灸杂志,2021,40(2):194-199.[5]李程,陈波,胡铁汉,等.不同规格毫针针刺对大鼠穴区筋膜组织、细胞和胶原纤维的影响[J].中国针灸,2015,35(8):801-805.[6]任超,鞠露,陈士炯,等.针刺背俞穴、夹脊穴治疗老年腰椎间盘突出症的临床观察[J].世界中西医结合杂志,2021,16(6):1147-1150.[7]Azraq I,Craveiro RB,Niederau C,et al.Gene expression and phosphorylation of ERK and AKT are regulated depending on mechanical force and cell confluence in murine cementoblasts[J].Ann Anat,2021(234):151668.[8]Tsai E,Casaccia P.Mechano-modulation of nuclear events regulating oligodendrocyte progenitor gene expression[J].Glia,2019,67(7):1229-1239.[9]Nikmanesh M,Cancel LM,Shi ZD,et al.Heparan sulfate proteoglycan, integrin, and syndecan-4 are mechanosensors mediating cyclic strain-modulated endothelial gene expression in mouse embryonic stem cell-derived endothelial cells[J].Biotechnol Bioeng,2019,116(10):2730-2741.[10]周艳朋,李长聪,焦雪蕾.针刺颈夹脊配合风池、后溪及束骨穴治疗颈性眩晕的疗效及对血流动力学的影响[J].中西医结合心脑血管病杂志,2020,18(4):664-667.[11]刘云逸,王博,王琳.运动后针刺腓肠肌干预肥胖大鼠肌腱退行性病变[J].中国组织工程研究,2021,25(14):2211-2218.[12]曹昺,焱饶毅,庄威,等.从骨骼肌张力传递解读反阿是穴的治疗效果[J].中国针灸,2021,41(2):217-220.[13]胡铁汉,江瑜,陈波,等.体外压力刺激对血管内皮细胞不同整合素亚基表达变化影响的研究[J].按摩与康复医学,2016,7(1):11-14.[14]魏凯,王春丽.“调理脾胃”针法联合风池穴针刺治疗颈性眩晕的疗效及对椎-基底动脉血流动力学影响[J].针灸临床杂志,2020,36(6):15-18.[15]Zhang C,Wang F,Gao Z,et al.Regulation of Hippo Signaling by Mechanical Signals and the Cytoskeleton[J].DNA Cell Biol,2019,39(2):5087-5093.[16]Chakraborty S,Jasnin M,Baumeister W.Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective[J].Protein Sci,2020,29(6):1302-1320.[17]徐丽萌.Anillin对细胞骨架蛋白和细胞力学特性的调节作用[D].太原:太原理工大学,2019.[18]Min YS,Yun NY,Song JH,et al.Functional activity of mitochondria associated with cytoskeleton stability in the HEK293[J].FASEB J,2020,34(S1):1-2.[19]Dieckman L.Something’s gotta give: How PCNA alters its structure in response to mutations and the implications on cellular processes[J].Prog Biophys and Mol Biol,2021(163):46-59.

相似文献/References:

[1]卓春萍.温针灸与针刺治疗老年性膝骨关节病的临床疗效对比[J].医学信息,2018,31(11):148.[doi:10.3969/j.issn.1006-1959.2018.11.048]
　ZHUO Chun-ping.Comparison of Clinical Effects of Warming Acupuncture and Acupuncture in the Treatment of Senile Knee Osteoarthropathy[J].Medical Information,2018,31(03):148.[doi:10.3969/j.issn.1006-1959.2018.11.048]
[2]井庆彦.针刺配合拔罐治疗单纯性肥胖的临床效果观察[J].医学信息,2018,31(16):139.[doi:10.3969/j.issn.1006-1959.2018.16.044]
　JING Qing-yan.Clinical Observation of Acupuncture Combined with Cupping in the Treatment of Simple Obesity[J].Medical Information,2018,31(03):139.[doi:10.3969/j.issn.1006-1959.2018.16.044]
[3]戴雪珍,熊 俊.针刺调神思想临床应用探析[J].医学信息,2019,32(08):72.[doi:10.3969/j.issn.1006-1959.2019.08.021]
　DAI Xue-zhen,XIONG Jun.Analysis of Clinical Application of Acupuncture and Soothe the Mind[J].Medical Information,2019,32(03):72.[doi:10.3969/j.issn.1006-1959.2019.08.021]
[4]江 明,张宏波.局部针刺联合中药包热敷治疗神经根型颈椎病的疗效[J].医学信息,2019,32(15):149.[doi:10.3969/j.issn.1006-1959.2019.15.049]
　JIANG Ming,ZHANG Hong-bo.Curative Effect of Local Acupuncture Combined with Chinese Herbal Medicine in Treatment of Cervical Spondylotic Radiculopathy[J].Medical Information,2019,32(03):149.[doi:10.3969/j.issn.1006-1959.2019.15.049]
[5]李 航,李 月.针刺配合吞咽康复训练治疗脑卒中后吞咽障碍的研究[J].医学信息,2019,32(18):160.[doi:10.3969/j.issn.1006-1959.2019.18.056]
　LI Hang,LI Yue.Acupuncture Combined with Swallowing Rehabilitation Training for Dysphagia After Stroke[J].Medical Information,2019,32(03):160.[doi:10.3969/j.issn.1006-1959.2019.18.056]
[6]徐新刚.肾脑复元汤联合针刺治疗缺血性中风的疗效[J].医学信息,2019,32(23):159.[doi:10.3969/j.issn.1006-1959.2019.23.050]
　XU Xin-gang.Therapeutic Effect of Shennao Fuyuan Decoction Combined with Acupuncture on Ischemic Stroke[J].Medical Information,2019,32(03):159.[doi:10.3969/j.issn.1006-1959.2019.23.050]
[7]潘穆之,李 园,何 炯.祛瘀化痰针刺法治疗中风后假性球麻痹的疗效[J].医学信息,2020,33(03):156.[doi:10.3969/j.issn.1006-1959.2020.03.051]
　PAN Mu-zhi,LI Yuan,HE Jiong.Effect of Acupuncture for Removing Blood Stasis and Resolving Phlegmin Treating Post-stroke Pseudobulbar Palsy[J].Medical Information,2020,33(03):156.[doi:10.3969/j.issn.1006-1959.2020.03.051]
[8]姚丽君,冯裕星,范文辉,等.针刺对脑卒中后首发焦虑障碍与下肢活动功能的影响[J].医学信息,2020,33(12):76.[doi:10.3969/j.issn.1006-1959.2020.12.022]
　YAO Li-jun,FENG Yu-xing,FAN Wen-hui,et al.Effect of Acupuncture on first-onset Anxiety Disorder and Lower Limb Activity After Stroke[J].Medical Information,2020,33(03):76.[doi:10.3969/j.issn.1006-1959.2020.12.022]
[9]杨慧琳,徐 丹,刁本恕.中医治疗孤独症谱系障碍的现状研究[J].医学信息,2020,33(16):42.[doi:10.3969/j.issn.1006-1959.2020.16.013]
　YANG Hui-lin,XU Dan,DIAO Ben-shu.Research on Current Situation of TCM Treatment of Autism Spectrum Disorder[J].Medical Information,2020,33(03):42.[doi:10.3969/j.issn.1006-1959.2020.16.013]
[10]黄渺苗,崔 燕.针刺治疗对合并焦虑状态的更年期女性室性期前收缩的影响[J].医学信息,2020,33(17):103.[doi:10.3969/j.issn.1006-1959.2020.17.030]
　HUANG Miao-miao,CUI Yan.The Effect of Acupuncture on Ventricular Premature Contraction in Menopausal Women with Anxiety[J].Medical Information,2020,33(03):103.[doi:10.3969/j.issn.1006-1959.2020.17.030]

更新日期/Last Update: 1900-01-01