[1]杨建伟.气相色谱质谱法在食品有机磷农药残留检测中的应用[J].医学信息,2022,35(11):154-156,168.[doi:10.3969/j.issn.1006-1959.2022.11.041]
　YANG Jian-wei.Application of Gas Chromatography-mass Spectrometry in Determination of Organophosphorus Pesticide Residues in Food[J].Medical Information,2022,35(11):154-156,168.[doi:10.3969/j.issn.1006-1959.2022.11.041]
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气相色谱质谱法在食品有机磷农药残留检测中的应用()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年11期
页码:
154-156,168
栏目:
诊疗技术
出版日期:
2022-06-01

文章信息/Info

Title:
Application of Gas Chromatography-mass Spectrometry in Determination of Organophosphorus Pesticide Residues in Food
文章编号:
1006-1959（2022）11-0154-04
作者:
杨建伟
（天津市宝坻区疾病预防控制中心理化检验科，天津 301800）
Author(s):
YANG Jian-wei
(Department of Physical and Chemical Laboratory,Baodi District Center for Disease Control and Prevention,Tianjin 301800,China)
关键词:
相色谱质谱法; 食品; 机磷农药; 检测; 残留
Keywords:
Gas chromatography-mass spectrometry; Food; Organophosphorus pesticide; Detection; Residue
分类号:
TS207.3
DOI:
10.3969/j.issn.1006-1959.2022.11.041
文献标志码:
A
摘要:
目的 研究气相色谱质谱法在食品有机磷农药残留检测中的应用。方法 采用气相色谱质谱法食品中有机磷农药的残留情况进行检测，并应用不同溶液萃取样品，对有机农药的残留种类、残留时间、丰度比以及特征离子进行观察。结果 采用乙腈提取米饭、水、生白菜、烧肉提取效率91.21%、92.08%、98.45%、103.34%，均高于二氯甲烷（81.02%、85.39%、89.06%、90.34%）、氯化钠（82.44%、87.44%、88.94%~92.98%）（P＜0.05）；0.2 mg/kg加标试验结果显示，米饭、水、生白菜、烧肉分别采用QuEChERS和Carb/NH2柱两种净化方式的回收率比较，差异无统计学意义（P＞0.05）；不同有机磷农药残留静置15 min时有机农药丰度比、特征离子高于静置12~14 min（P＜0.05）；有机磷的检测方法的线性范围为0.1~5.0 μg/ml，线性相关系数为0.98~0.99，检出限为0.002~0.045 mg/kg；气相色谱质谱法检测白菜、食用油有机磷回收率均高于82.30%，相对标准偏差均处于2.60%~16.70%。结论 气相色谱质谱法操作简单快速，采用乙腈萃取样品，静置时间15 min，检测具有较高的精确度、灵敏度，可满足食品有机磷农药残留检测的需求。
Abstract:
Objective To study the application of gas chromatography-mass spectrometry in the detection of organophosphorus pesticide residues in food.Methods The residues of organophosphorus pesticides in food were detected by gas chromatography-mass spectrometry, and the samples were extracted with different solutions to observe the residual types, residual time, abundance ratio and characteristic ions of organic pesticides.Results The extraction efficiencies of rice, water, lettuce and roasted meat with acetonitrile were 91.21%, 92.08%, 98.45% and 103.34%, which were higher than those of dichloromethane (81.02%, 85.39%, 89.06% and 90.34%), sodium chloride (82.44%, 87.44% and 88.94%-92.98%) (P<0.05). The results of 0.2 mg/kg standard addition test showed that there was no significant difference in the recovery rates of rice, water, lettuce and roasted meat by QuEChERS and Carb/NH2 column purification methods (P>0.05). The abundance ratio of organic pesticides and characteristic ions of different organophosphorus pesticide residues at 15 min were higher than those at 12-14 min (P<0.05). The linear range of the detection method for organophosphorus was 0.1-5.0 μg/ml, the linear correlation coefficient was 0.98-0.99, and the detection limit was 0.002-0.045 mg/kg. The recovery rates of organic phosphorus in cabbage and edible oil detected by GC-MS were higher than 82.30%, and the relative standard deviations were in the range of 2.60%-16.70%.Conclusion Gas chromatography-mass spectrometry is simple and rapid. The sample is extracted with acetonitrile and the detection time is 15 min, which has high accuracy and sensitivity, and can meet the needs of food organophosphorus pesticide residue detection.

参考文献/References:

[1]Farajzadeh MA,Asghari A,Feriduni B.An efficient,rapid and microwave-accelerated dispersive liquid-liquid microextraction method for extraction and pre-concentration of some organophos-phorus pesticide residues from aqueous samples[J].Journal of Food Composition and Analysis,2016,4873-4880.[2]王海萍,王红玲,陈建文,等.QuEchERS-气相色谱-质谱法同时测定果蔬中23种农药残留[J].中国卫生检验杂志,2017,27(9):1250-1252,1258.[3]田菲菲,张曦,马金凤,等.气相色谱-串联质谱法同时分析葡萄基质中196种农药残留[J].食品安全质量检测学报,2016,7(3):1069-1081.[4]耿慧春,梅文泉,汪禄祥,等.固相萃取-气相色谱—电子捕获检测器测定蔬菜中11种有机磷农药残留[J].食品安全质量检测学报,2018,9(1):116-122.[5]中华人民共和国国家质量监督检验检疫总局.GB/T27418-2017测量不确定度评定和表示[S].北京:中国标准出版社,2017.[6]中国合格评定国家认可委员会.CNAS-CL01检测和校准实验室能力认可准则[S].北京:中国计量出版社,2018.[7]唐娜.2017-2018年济宁城区市售食品中有机磷农药残留量监测分析[J].检验检疫学刊,2019,29(2):44-45.[8]张雷,曹淑瑞,谭斯匀,等.高效液相色谱法快速检测茶叶中三氯杀螨醇[J].分析试验室,2017,36(9):1048-1052.[9]王兵,李斌,张媛媛,等.QuEchERS-高效液相色谱法测定绿茶中虫螨腈残留[J].中国食品学报,2017,17(1):240-245.[10]王吉祥,牛之瑞,冯雷,等.SPE-GC-MS/MS法测定茶叶中49种农药残留[J].食品研究与开发,2017,38(13):173-178.[11]苏建峰,钟茂生,陈晶,等.气相色谱-质谱法与气相色谱法测定茶叶及茶叶加工品中295种农药残留[J].分析测试学报,2015,34(6):625-638.[12]容秀湾,李慧玲,杨帆.气相色谱法在食品农药残留检测中的应用[J].现代食品,2019(5):117-119.[13]胡红美,郭远明,金衍健,等.固相膜萃取-气相色谱法测定养殖用水中痕量有机磷农药[J].理化检验(化学分册),2015,51(6):770-774.[14]王芹,杭学宇,宋鑫,等.气相色谱/质谱法检测食物中毒样品中23种有机磷类农药[J].环境卫生学杂志,2019,9(2):172-177,183.[15]谭珊,蔡小钦,李红丽,等.气相色谱质谱检测食品中有机磷农药残留分析[J].生物化工,2019,5(2):91-92.[16]张殿伟,王金菊,王艳萍.量子点传感体系在有机磷农药残留检测中的应用[J].食品研究与开发,2019,40(1):214-219.[17]李婧,俞寅,王丽娟,等.超高效液相色谱-串联质谱法测定谷物和油料中10种有机磷农药残留[J].粮食科技与经济,2020,45(6):81-84.[18]何娜.气相色谱法对测定大米中的有机磷农药残留含量测定[J].江西农业,2018(12):41.[19]司晓喜,朱瑞芝,张凤梅,等.微波辅助萃取-气相色谱-负化学电离/电子轰击电离飞行时间质谱法测定烟草中农药残留[J].色谱,2016,34(6):608-614.[20]邵天时.气相色谱质谱法检测食品中有机磷农药残留分析[J].质量安全与检验检测,2020,30(6):44-45.

更新日期/Last Update: 1900-01-01