[1]蒋漪桦,朱 宇,杜洪灵,等.Lnc RNA TUC338影响人类宫颈癌细胞的蛋白组学分析[J].医学信息,2022,35(13):18-27.[doi:10.3969/j.issn.1006-1959.2022.13.004]
　JIANG Yi-hua,ZHU Yu,DU Hong-ling,et al.Proteomics Analysis of Lnc RNA TUC338 Affecting Human Cervical Cancer Cells[J].Medical Information,2022,35(13):18-27.[doi:10.3969/j.issn.1006-1959.2022.13.004]
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Lnc RNA TUC338影响人类宫颈癌细胞的蛋白组学分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年13期
页码:
18-27
栏目:
生物信息学
出版日期:
2022-07-01

文章信息/Info

Title:
Proteomics Analysis of Lnc RNA TUC338 Affecting Human Cervical Cancer Cells
文章编号:
1006-1959（2022）13-0018-10
作者:
蒋漪桦; 朱 宇; 杜洪灵; 等
（1.南华大学衡阳医学院，湖南 衡阳 421001；2.上海市普陀区人民医院妇产科，上海 200060；3.上海市普陀区人民医院检验科，上海 200060；4.上海市普陀区人民医院中心实验室，上海 200060）
Author(s):
JIANG Yi-hua; ZHU Yu; DU Hong-ling; et al.
(1.Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;2.Department of Gynecology and Obstetrics,Putuo District People’s Hospital,Shanghai200060,China;3.Department of Laboratory,Putuo District People’s Hospital,Shanghai200060,China;4.Department of Clinical Laboratory,Putuo District People’s Hospital,Shanghai 200060,China)
关键词:
宫颈癌; 长链非编码RNA TUC338; 非标记定量法; 蛋白组学
Keywords:
Cervical cancer; Long-chain noncoding RNA TUC338; Label-free; Proteomics
分类号:
R737.33
DOI:
10.3969/j.issn.1006-1959.2022.13.004
文献标志码:
A
摘要:
目的 探究长链非编码RNA TUC338影响人类宫颈癌细胞的分子机制。方法 将人类宫颈癌细胞Hela细胞分为实验组（TUC338组）与对照组（rLV组），分别感染rLV-hTUC338-ZsGreen-Puro慢病毒及rLV-ZsGreen-Puro慢病毒，RT-PCR检测两组细胞Lnc RNA TUC338表达量，并对两组细胞进行蛋白质谱分析。结果 感染慢病毒后所有细胞均有绿色荧光显示，RT-PCR结果显示TUC338组Lnc RNA TUC338表达量较rLV组增加，两组均显示较多的蛋白条带；非标记定量法蛋白测序共检测到4323个蛋白。蛋白差异分析显示：与rLV组比较，在TUC338组中下调的蛋白有194个，上调的蛋白有225个。其中，重要的下调蛋白有：LAMB1、HNRNPH3、COX2等，上调蛋白有：EPPK1、ITPR1、AKR1C2等。最显著的下调GO为磷酸化修饰功能，上调GO为细胞骨架功能，最显著的下调KEGG通路为半乳糖代谢通路，上调KEGG通路为肌动蛋白细胞骨架调节通路。共检测到含WD40 repeat结构域（WD40 repeat）等20个蛋白结构域。rLV组和rLV-TUC338组中有显著差异的结构域包括Haem peroxidase，animal等。主要亚细胞成分及主要差异亚细胞成分均为核蛋白，分别占34.23%、24.22%。rLV组和rLV-TUC338组中下调的互作网主要包括：prot1（P37268）-prot2（O95864）、prot1（Q6FGH9）-prot2（Q13363）等。上调的互作网主要包括：prot1（P60520）-prot2（A0A024RBQ5）、prot1（P60520）-prot2（Q06330）等。结论 Lnc RNA TUC338影响了人类宫颈癌细胞的蛋白组学，是宫颈癌的抑癌基因，并通过糖代谢、磷酸化、细胞骨架这3个通路抑制肿瘤发展。
Abstract:
Objective To explore the molecular mechanism of long-chain non-coding RNA TUC338 affecting human cervical cancer cells.Methods Human cervical cancer Hela cells were divided into experimental group (TUC338 group) and control group (rLV group). rLV-hTUC338-ZsGreen-Puro lentivirus and rLV-ZsGreen-Puro lentivirus were transfected respectively. The expression of Lnc RNA TUC338 in the two groups was detected by RT-PCR, and the protein spectrum of the two groups was analyzed.Results All cells were fluorescently green after infection with lentivirus. The results of RT-PCR showed that the expression of Lnc RNA TUC338 in TUC338 group was higher than that in rLV group, and both groups showed more protein bands. A total of 4323 proteins were detected by non-labeled quantitative protein sequencing. Protein difference analysis showed that compared with the rLV group, 194 proteins were down-regulated and 225 proteins were up-regulated in the TUC338 group. Among them, the important down-regulated proteins were LAMB1, HNRNPH3, COX2 and up-regulated proteins were EPPK1, ITPR1, AKR1C2 and so on. The most significant down-regulation of GO was phosphorylation modification function, and up-regulation of GO was cytoskeleton function. The most significant down-regulation of KEGG pathway was galactose metabolism pathway, and up-regulation of KEGG pathway was actin cytoskeleton regulation pathway. A total of 20 protein domains including WD40 repeat domain were detected. In rLV group and rLV-TUC338 group, there were significant differences in domains including Haem peroxidase, animals, etc. The main subcellular components and the main differential subcellular components were nuclear proteins, accounting for 34.23% and 24.22%, respectively. The down-regulated interaction network in rLV group and rLV-TUC338 group mainly included: prot1(P37268)-prot2 (O95864), prot1(Q6FGH9)-prot2 (Q13363), etc. The up-regulated interaction network mainly included: prot1(P60520)-prot2 (A0A024RBQ5), prot1(P60520)-prot2(Q06330), etc.Conclusion Lnc RNA TUC338 affects the proteomics of human cervical cancer cells, is a tumor suppressor gene of cervical cancer, and inhibits tumor development through glucose metabolism, phosphorylation and cytoskeleton.

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更新日期/Last Update: 1900-01-01