[1]张 博,张 浩.MicroRNA-744调控PRKACA和FGF2对皮肤烧伤愈后瘢痕形成的影响[J].医学信息,2022,35(16):83-85.[doi:10.3969/j.issn.1006-1959.2022.16.019]
 ZHANG Bo,ZHANG Hao.Effect of MicroRNA-744 Regulating PRKACA and FGF2 on Scar Formation After Skin Burn Wound Healing[J].Journal of Medical Information,2022,35(16):83-85.[doi:10.3969/j.issn.1006-1959.2022.16.019]
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MicroRNA-744调控PRKACA和FGF2对皮肤烧伤愈后瘢痕形成的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年16期
页码:
83-85
栏目:
论著
出版日期:
2022-08-15

文章信息/Info

Title:
Effect of MicroRNA-744 Regulating PRKACA and FGF2 on Scar Formation After Skin Burn Wound Healing
文章编号:
1006-1959(2022)16-0083-03
作者:
张 博张 浩
(佳木斯大学附属第一医院整形外科,黑龙江 佳木斯 154007)
Author(s):
ZHANG BoZHANG Hao
(Department of Plastic Surgery,the First Affiliated Hospital of Jiamusi University,Jiamusi 154007,Heilongjiang,China)
关键词:
MicroRNA-744蛋白激酶cAMP激活催化亚基α成纤维细胞生长因子2皮肤烧伤
Keywords:
MicroRNA-744Protein kinase cAMP-activated catalytic subunit alphaFibroblast growth factor 2Skin burns
分类号:
R644
DOI:
10.3969/j.issn.1006-1959.2022.16.019
文献标志码:
A
摘要:
目的 研究皮肤烧伤愈后瘢痕形成中MicroRNA-744调控蛋白激酶cAMP激活催化亚基α(PRKACA)和成纤维细胞生长因子2(FGF2)的作用和相关性。方法 选择烧伤动物模型共30只作为研究对象,分为对照组、烧伤组、愈后组,每组10只。比较各组microRNA-744表达、PRKACA和FGF2水平及蛋白表达量,并采用Pearson相关性分析microRNA-744与PRKACA和FGF2的关系。结果 烧伤组和愈后组microRNA-744表达量分别为(6.901±1.031)、(7.583±1.067),均高于对照组的(4.671±1.012),差异有统计学意义(P<0.05);烧伤组和愈后组PRKACA表达水平及蛋白表达低于对照组,且愈后组低于烧伤组,差异有统计学意义(P<0.05);烧伤组和愈后组FGF2表达水平及蛋白表达高于对照组,且愈后组高于烧伤组,差异有统计学意义(P<0.05);Pearson相关性分析显示,microRNA-744和PRKACA呈负相关(r=-0.821,P<0.05),与FGF2呈正相关(r=0.773,P<0.05)。结论 在皮肤烧伤愈后瘢痕形成中microRNA-744可以调控PRKACA和FGF2的表达,其通过调控PRKACA间接促进FGF2的表达。
Abstract:
Objective To study the role and correlation of microRNA-744 regulating protein kinase cAMP-activated catalytic subunit alpha (PRKACA) and fibroblast growth factor 2 (FGF2) in scar formation after skin burn.Methods A total of 30 burn animal models were selected as the research objects and divided into control group, burn group and recovery group, with 10 rats in each group. The expression of microRNA-744, PRKACA and FGF2 levels and protein expression were compared among the groups, and the relationship between microRNA-744 and PRKACA and FGF2 was analyzed by Pearson correlation.Results The expression levels of microRNA-744 in the burn group and the healing group were (6.901±1.031) and (7.583±1.067), respectively, which were higher than (4.671±1.012) in the control group, and the differences were statistically significant (P<0.05). The expression level and protein expression of PRKACA in the burn group and the healing group were lower than those in the control group, and the healing group was lower than the burn group (P<0.05). The expression level and protein expression of FGF2 in the burn group and the healing group were higher than those in the control group, and the healing group was higher than the burn group, the differences were statistically significant (P<0.05). Pearson correlation analysis showed that microRNA-744 was negatively correlated with PRKACA (r=-0.821, P<0.05) and positively correlated with FGF2 (r=0.773, P<0.05).Conclusion MicroRNA-744 can regulate the expression of PRKACA and FGF2 in scar formation after skin burn healing, which indirectly promotes the expression of FGF2 by regulating PRKACA.

参考文献/References:

[1]Roshangar L,Soleimani Rad J,Kheirjou R,et al.Skin Burns: Review of Molecular Mechanisms and Therapeutic Approaches[J].Wounds,2019,31(12):308-315.[2]梁娜娜,薛喜娟,刘晓慧,等.烧伤患者照顾者生活质量及其影响因素[J].河南医学研究,2021,30(31):5769-5773.[3]Barnes LA,Marshall CD,Leavitt T,et al.Mechanical Forces in Cutaneous Wound Healing: Emerging Therapies to Minimize Scar Formation[J].Adv Wound Care (New Rochelle),2018,7(2):47-56.[4]Savoji H,Godau B,Hassani MS,et al.Skin Tissue Substitutes and Biomaterial Risk Assessment and Testing[J].Front Bioeng Biotechnol,2018,6:86.[5]Gibson ALF,Carney BC,Cuttle L,et al.Coming to Consensus: What Defines Deep Partial Thickness Burn Injuries in Porcine Models?[J].J Burn Care Res,2021,42(1):98-109.[6]Zhang M,Li H,Zhang Y,et al.Oncogenic miR-744 promotes prostate cancer growth through direct targeting of LKB1[J].Oncol Lett,2019,17(2):2257-2265.[7]Kang W,Huang T,Zhou Y,et al.miR-375 is involved in Hippo pathway by targeting YAP1/TEAD4-CTGF axis in gastric carcinogenesis[J].Cell Death Dis,2018,9(2):92. [8]Moody SE,Schinzel AC,Singh S,et al.PRKACA mediates resistance to HER2-targeted therapy in breast cancer cells and restores anti-apoptotic signaling[J].Oncogene,2015,34(16):2061-2071.[9]Jimenez-Pascual A,Mitchell K,Siebzehnrubl FA,et al.FGF2:a novel druggable target for glioblastoma?[J].Expert Opin Ther Targets,2020,24(4):311-318.[10]胡雅琼,白俊,陈琳,等.miR-625-5p通过靶向调控PRKACA促进肺腺癌细胞的增殖和侵袭[J].中国癌症杂志,2021,31(6):447-454.[11]Yu W,Chen PB,Chen FC,et al.MicroRNA-744 promotes proliferation of osteosarcoma cells by targeting PTEN[J].Mol Med Rep,2020,21(5):2276-2282.[12]Ahn HN,Kang HS,Park SJ,et al.Safety and efficacy of basic fibroblast growth factors for deep second-degree burn patients[J].Burns,2020,46(8):1857-1866.

更新日期/Last Update: 1900-01-01