[1]罗瑶敏,蒋 振.长链非编码 RNA-MAL2的靶基因预测及信号通路富集分析[J].医学信息,2022,35(19):11-14.[doi:10.3969/j.issn.1006-1959.2022.19.003]
　LUO Yao-min,JIANG Zhen.Target Gene Prediction and Signal Pathway Enrichment Analysis of Long Non-coding RNA-MAL2[J].Journal of Medical Information,2022,35(19):11-14.[doi:10.3969/j.issn.1006-1959.2022.19.003]
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长链非编码 RNA-MAL2的靶基因预测及信号通路富集分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年19期
页码:
11-14
栏目:
生物信息学
出版日期:
2022-10-01

文章信息/Info

Title:
Target Gene Prediction and Signal Pathway Enrichment Analysis of Long Non-coding RNA-MAL2
文章编号:
1006-1959（2022）19-0011-04
作者:
罗瑶敏; 蒋 振
（川北医学院基础医学与法医学院生物化学与分子生物学教研室，四川 南充 637000）
Author(s):
LUO Yao-min; JIANG Zhen
(Department of Biochemistry and Molecular Biology,School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
关键词:
lncRNA-MAL2; 调控靶基因; 细胞迁移; 蛋白泛素化
Keywords:
lncRNA-MAL2; Regulating target genes; Cell migration; Protein ubiquitination
分类号:
Q343.1；Q811.4
DOI:
10.3969/j.issn.1006-1959.2022.19.003
文献标志码:
A
摘要:
目的 运用生物信息学分析方法，分析长链非编码RNA-MAL2（lncRNA-MAL2）在细胞内的调控靶基因及信号通路，并对其进行生物学功能注释，阐述lncRNA-MAL2在细胞内的功能作用。方法 在miRDB数据库和Starbase3.0数据库中预测与lncRNA-MAL2有miRNA-lncRNA互作关系的miRNA，将结果取交集；在Starbase3.0数据库中预测miRNAs靶基因，并进行GO功能注释和Pathway通路富集。结果 在miRDB数据库预测到95个miRNAs与lncRNA-MAL2相作用， Starbase3.0数据库预测到167个miRNAs与之相作用，取2个数据库的交集共获得5个miRNAs；GO功能注释显示其靶基因主要分布在镁离子结合、酶结合、蛋白激酶、蛋白泛素化等方面；生物学过程主要分布在细胞迁移、抑制细胞增殖、凋亡、蛋白转运和细胞周期等功能方面；信号通路富集主要分布在ErbB信号通路、生长激素合成、TNF信号通路、TGF-beta信号通路及雌激素信号通路等方面。结论 lncRNA-MAL2在细胞内的重要调控作用具体与酶结合、蛋白激酶、蛋白泛素化、细胞迁移、抑制细胞增殖、凋亡、蛋白转运和细胞周期等多条信号通路密切相关。
Abstract:
Objective To analyze the regulatory target genes and signaling pathways of long non-coding RNA-MAL2(lncRNA-MAL2) in cells by bioinformatics analysis, and annotate its biological functions, so as to elaborate the functions of lncRNA-MAL2 in cells.Methods The miRNAs interacting with lncRNA-MAL2 were predicted in miRDB database and Starbase3.0 database, and the results were intersected. Target genes of miRNAs were predicted in the Starbase 3.0 database, and GO functional annotation and Pathway enrichment were performed.Results Ninety-five miRNAs were predicted to interact with lncRNA-MAL2 in the miRDB database, and 167 miRNAs were predicted to interact with lncRNA-MAL2 in the Starbase3.0 database. A total of 5 miRNAs from the intersection of the 2 databases were selected for further analysis. GO functional annotation showed that its target genes were mainly distributed in magnesium ion binding, enzyme binding, protein kinase, protein ubiquitination, etc. Biological processes are mainly distributed in cell migration, inhibition of cell proliferation, apoptosis, protein transport and cell cycle. Signal pathway enrichment was mainly distributed in ErbB signaling pathway, growth hormone synthesis, TNF signaling pathway, TGF-beta signaling pathway and estrogen signaling pathway.Conclusion The important regulatory role of lncRNA-MAL2 in cells is closely related to multiple signaling pathways such as enzyme binding, protein kinase, protein ubiquitination, cell migration, inhibition of cell proliferation, apoptosis, protein transport and cell cycle.

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更新日期/Last Update: 1900-01-01