[1]袁晓霞,蒋 振.长链非编码RNA-Keratin 8的调控通路分析[J].医学信息,2022,35(19):6-10.[doi:10.3969/j.issn.1006-1959.2022.19.002]
　YUAN Xiao-xia,JIANG Zhen.Regulatory Pathway Analysis of Long Non-coding RNA-Keratin 8[J].Journal of Medical Information,2022,35(19):6-10.[doi:10.3969/j.issn.1006-1959.2022.19.002]
点击复制

长链非编码RNA-Keratin 8的调控通路分析()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年19期
页码:
6-10
栏目:
生物信息学
出版日期:
2022-10-01

文章信息/Info

Title:
Regulatory Pathway Analysis of Long Non-coding RNA-Keratin 8
文章编号:
1006-1959（2022）19-0006-05
作者:
袁晓霞; 蒋 振
（川北医学院基础医学与法医学院生物化学与分子生物学教研室，四川 南充 637000）
Author(s):
YUAN Xiao-xia; JIANG Zhen
(Department of Biochemistry and Molecular Biology,School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
关键词:
lncRNA-KRT8; 肿瘤; 靶基因; 信号通路
Keywords:
lncRNA-KRT8; Tumor; Target genes; Signal pathway
分类号:
Q343.1；Q811.4
DOI:
10.3969/j.issn.1006-1959.2022.19.002
文献标志码:
A
摘要:
目的 运用生物信息学分析方法，分析长链非编码RNA-细胞角蛋白8（KRT8）在细胞内的调控靶基因及信号通路，并对其进行生物学功能注释，阐述lncRNA-KRT8在细胞内的功能作用。方法 在miRDB数据库和Starbase3.0数据库中预测与lncRNA-KRT8有miRNA-lncRNA互作关系的miRNA，将结果取交集；在Starbase3.0数据库中预测miRNAs靶基因，并进行GO功能注释和Pathway通路富集。结果 在miRDB数据库预测到15个miRNAs与lncRNA-KRT8相作用，Starbase3.0数据库预测到60个miRNAs与之相作用，取2个数据库的交集共获得2个miRNAs；GO功能注释显示lncRNA-KRT8通过hsa-miR-150-5p作用的靶基因主要调控蛋白结合、RNA结合、金属离子结合信号通路、转录调控作用及蛋白质的磷酸化过程；通过hsa-miR-512-3p作用的靶基因主要调控蛋白结合、DNA结合、金属离子的结合信号通路、蛋白质的泛素化降解过程；通过hsa-miR-150-5p主要调控ErbB信号通路、肿瘤中糖蛋白信号通路、miRNAs、胃癌信号通路等；通过hsa-miR-512-3p作用的信号通路主要是乙型肝炎、人T细胞白血病病毒、前列腺癌、胰岛素抵抗和MAPK信号通路等。结论 长链非编码RNA-KRT8在细胞内的调控作用具体与蛋白质的结合和金属离子结合及糖蛋白、脂代谢信号通路密切相关，其可通过上述作用通路调控疾病的病理进展。
Abstract:
Objective To analyze the regulatory target genes and signaling pathways of long non-coding RNA-cytokeratin 8 (KRT8) in cells by bioinformatics analysis, and to annotate its biological functions, so as to elucidate the functional role of lncRNA-KRT8 in cells.Methods The miRNAs interacting with lncRNA-KRT8 were predicted in miRDB database and Starbase3.0 database, and the results were intersected. Target genes of miRNAs were predicted in the Starbase 3.0 database, and GO functional annotation and Pathway enrichment were performed.Results A total of 15 miRNAs were predicted to interact with lncRNA-KRT8 in miRDB database, and 60 miRNAs were predicted to interact with lncRNA-KRT8 in Starbase database. A total of 2 miRNAs from the intersection of the two databases were selected for further analysis. GO functional annotation showed that the target genes of long non-coding RNA KRT8 through hsa-miR-150-5p mainly regulated protein binding, RNA binding, metal ion binding signaling pathway, transcriptional regulation and protein phosphorylation. The target genes of hsa-miR-512-3p mainly regulate protein binding, DNA binding, metal ion binding signaling pathway, protein ubiquitination degradation process ; hsa-miR-150-5p mainly regulates ErbB signaling pathway, tumor glycoprotein signaling pathway, miRNAs, gastric cancer signaling pathway, etc. The signaling pathways through hsa-miR-512-3p are mainly hepatitis B, human T cell leukemia virus, prostate cancer, insulin resistance and MAPK signaling pathways.Conclusion The regulation of long non-coding RNA-KRT8 in cells is closely related to protein binding and metal ion binding and glycoprotein and lipid metabolism signaling pathways, which can regulate the pathological progression of diseases through the above-mentioned pathways.

参考文献/References:

[1]Ali T,Grote P.Beyond the RNA-dependent function of LncRNA genes[J].Elife,2020,9:e60583.[2]Bridges MC,Daulagala AC,Kourtidis A.LNCcation: lncRNA localization and function[J].J Cell Biol,2021,220(2):e202009045.[3]Ferrè F,Colantoni A,Helmer-Citterich M.Revealing protein-lncRNA interaction[J].Brief Bioinform,2016,17(1):106-116.[4]Jathar S,Kumar V,Srivastava J,et al.Technological Developments in lncRNA Biology[J].Adv Exp Med Biol,2017,1008:283-323.[5]Robinson EK,Covarrubias S,Carpenter S.The how and why of lncRNA function:An innate immune perspective[J].Biochim Biophys Acta Gene Regul Mech,2020,1863(4):194419.[6]Paraskevopoulou MD,Hatzigeorgiou AG.Analyzing MiRNA-LncRNA Interactions[J].Methods Mol Biol,2016,1402:271-286.[7]Walker LC,Harris GC,Holloway AJ,et al.Cytokeratin KRT8/18 expression differentiates distinct subtypes of grade 3 invasive ductal carcinoma of the breast[J].Cancer Genet Cytogenet,2007,178(2):94-103.[8]Stanke F,Hedtfeld S,Becker T,et al.An association study on contrasting cystic fibrosis endophenotypes recognizes KRT8 but not KRT18 as a modifier of cystic fibrosis disease severity and CFTR mediated residual chloride secretion[J].BMC Med Genet,2011,12:62.[9]Magalh?觔es-Novais S,Bermejo-Millo JC,Loureiro R,et al.Cell quality control mechanisms maintain stemness and differentiation potential of P19 embryonic carcinoma cells[J].Autophagy,2020,16(2):313-333.[10]Thorolfsdottir RB,Sveinbjornsson G,Aegisdottir HM,et al.DBDS Genomic Consortium[J].Eur Heart J,2021,42(20):1959-1971.[11]Park ES,Xiang D,Xie Y,et al.Oncogenic Events Dictate the Types and Locations of Gynecological Malignancies Originating from Krt8+ Mesothelial and Müllerian-Derived Epithelial Cells[J].Cancers (Basel),2022,14(3):841.[12]Schleiss MR.Letermovir and HCT: too much of a good thing?[J].Blood,2021,138(1):1-2.[13]Chan ACY,Wong HY,Chong YF,et al.Novel Autoantibodies in Idiopathic Small Fiber Neuropathy[J].Ann Neurol,2022,91(1):66-77.[14]Murray KA,Hughes MP,Hu CJ,et al.Identifying amyloid-related diseases by mapping mutations in low-complexity protein domains to pathologies[J].Nat Struct Mol Biol,2022,29(6):529-536.[15]Strnad P,Lienau TC,Tao GZ,et al.Denaturing temperature selection may underestimate keratin mutation detection by DHPLC[J].Hum Mutat,2006,27(5):444-452.[16]Kacham S,Bhure TS,Eswaramoorthy SD,et al.Human Umbilical Cord-Derived Mesenchymal Stem Cells Promote Corneal Epithelial Repair In Vitro[J].Cells,2021,10(5):1254.[17]Jian F,Hao W,Yun L,et al.High KRT8 expression promotes tumor progression and metastasis of Gastric Cancer[J].Cancer Sci,2017,108:178-186.[18]Tan HS,Jiang WH,He Y,et al.KRT8 upregulation promotes tumor metastasis and is predictive of a poor prognosis in clear cell renal cell carcinoma[J].Oncotarget,2017,76189-76203.[19]Guo D,Xu Q,Pabla S,et al.Cytokeratin-8 in anaplastic thyroid carcinoma: More than a simple structural cytoskeletal protein[J].Int J Mol Sci,2018,19:577.[20]Golob-Schwarzl N,Bettermann K,Mehta AK,et al.High keratin 8/18 ratio predicts aggressive hepatocellular cancer phenotype[J].Transl Oncol,2018,12:256-268.

相似文献/References:

[1]杨森果,毛大华,杨海松,等.E-cadherin与肿瘤侵袭、转移相关性研究进展[J].医学信息,2018,31(03):3.[doi:10.3969/j.issn.1006-1959.2018.04.002]
　YANG Sen-guo,MAO Da-hua,YANG Hai-song,et al.Research Progress of the Correlation between E-cadherin and Tumor Invasion and Metastasis[J].Journal of Medical Information,2018,31(19):3.[doi:10.3969/j.issn.1006-1959.2018.04.002]
[2]江立基,李先明.碳酸酐酶Ⅸ在肿瘤中的研究进展[J].医学信息,2018,31(03):7.[doi:10.3969/j.issn.1006-1959.2018.03.003]
　JIANG Li-ji,LI Xian-ming.Research Progress of Carbonic Anhydrase IX in Cancer[J].Journal of Medical Information,2018,31(19):7.[doi:10.3969/j.issn.1006-1959.2018.03.003]
[3]靳雪芹,邓正聪.代谢综合征与肿瘤关系的研究进展[J].医学信息,2018,31(05):35.[doi:10.3969/j.issn.1006-1959.2018.05.014]
　JIN Xue-qin,DENG Zheng-cong.Research Progress on the Relationship between Metabolic Syndrome and Tumor[J].Journal of Medical Information,2018,31(19):35.[doi:10.3969/j.issn.1006-1959.2018.05.014]
[4]白 雪,邓 琦.血液系统肿瘤的细胞治疗研究进展[J].医学信息,2018,31(10):41.[doi:10.3969/j.issn.1006-1959.2018.10.013]
　BAI Xue,DENG Qi.Advances in Cell Therapy for Blood System Tumor[J].Journal of Medical Information,2018,31(19):41.[doi:10.3969/j.issn.1006-1959.2018.10.013]
[5]李 轩,王子为,赵靖萱,等.肿瘤预后预测领域机器学习应用的文献计量与热点可视化分析[J].医学信息,2022,35(09):90.[doi:10.3969/j.issn.1006-1959.2022.09.023]
　LI Xuan,WANG Zi-wei,ZHAO Jing-xuan,et al.Visualization Analysis on Bibliometrics and Hot Spots of Machine Learning Applications in the Field of Tumor Prognosis Prediction[J].Journal of Medical Information,2022,35(19):90.[doi:10.3969/j.issn.1006-1959.2022.09.023]
[6]王 静,刘 梅,焦菊凤.知-信-行健康教育模式对PICC置管肿瘤 患者自我护理能力的影响[J].医学信息,2018,31(11):170.[doi:10.3969/j.issn.1006-1959.2018.11.056]
　WANG Jing,LIU Mei,JIAO Ju-feng.Effect of Knowledge-belief-behavioral Health Education Model on the Self-care Ability of Patients with Cancer in PICC Catheterization[J].Journal of Medical Information,2018,31(19):170.[doi:10.3969/j.issn.1006-1959.2018.11.056]
[7]史红娟,李群锋,赵 华,等.舒林酸抗肿瘤作用的研究进展[J].医学信息,2018,31(12):35.[doi:10.3969/j.issn.1006-1959.2018.12.012]
　SHI Hong-juan,LI Qun-feng,ZHAO Hua,et al.Advances in Research on Anti-tumor Effect of Sulindac[J].Journal of Medical Information,2018,31(19):35.[doi:10.3969/j.issn.1006-1959.2018.12.012]
[8]庞小坤,韩 梅,江心灿,等.当归补血汤治疗肿瘤化疗不良反应随机对照临床试验的Meta分析[J].医学信息,2019,32(02):69.[doi:10.3969/j.issn.1006-1959.2019.02.021]
　Pang Xiao-kun,Han Mei,Jiang Xin-can,et al.Meta-analysis of a Randomized Controlled Clinical Trial of Angelica Buxue Decoction in the Treatment of Adverse Reactions to Chemotherapy[J].Journal of Medical Information,2019,32(19):69.[doi:10.3969/j.issn.1006-1959.2019.02.021]
[9]张 慧,胡广越,殷 红.菌群干预与免疫治疗的研究进展[J].医学信息,2022,35(11):54.[doi:10.3969/j.issn.1006-1959.2022.11.016]
　ZHAHG Hui,HU Guang-yue,YIN Hong.Research Progress in Microbial Intervention and Immunotherapy[J].Journal of Medical Information,2022,35(19):54.[doi:10.3969/j.issn.1006-1959.2022.11.016]
[10]郑文灿,法艳梅,吕亚青,等.苦参碱抗肿瘤机制的分子生物学研究进展[J].医学信息,2018,31(19):51.[doi:10.3969/j.issn.1006-1959.2018.19.017]
　ZHENG Wen-can,FA Yan-mei,LV Ya-qing,et al.Advances in Molecular Biology of Matrine Antitumor Mechanism[J].Journal of Medical Information,2018,31(19):51.[doi:10.3969/j.issn.1006-1959.2018.19.017]

更新日期/Last Update: 1900-01-01