[1]魏 晨,蒋 振.长链非编码RNA-Hexokinase 2的生物信息学分析[J].医学信息,2022,35(19):1-5,10.[doi:10.3969/j.issn.1006-1959.2022.19.001]
　WEI Chen,JIANG Zhen.Bioinformatics Analysis of Long Non-coding RNA-Hexokinase 2[J].Journal of Medical Information,2022,35(19):1-5,10.[doi:10.3969/j.issn.1006-1959.2022.19.001]
点击复制

长链非编码RNA-Hexokinase 2的生物信息学分析()
分享到：

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年19期
页码:
1-5,10
栏目:
生物信息学
出版日期:
2022-10-01

文章信息/Info

Title:
Bioinformatics Analysis of Long Non-coding RNA-Hexokinase 2
文章编号:
1006-1959（2022）19-0001-06
作者:
魏 晨; 蒋 振
（川北医学院基础医学与法医学院生物化学与分子生物学教研室，四川 南充 637000）
Author(s):
WEI Chen; JIANG Zhen
(Department of Biochemistry and Molecular Biology,School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
关键词:
长链非编码RNA-Hexokinase 2; 信号通路; 蛋白磷酸化
Keywords:
Long non-coding RNA-Hexokinase 2; Signal pathway; Protein phosphorylation
分类号:
Q343.1；Q811.4
DOI:
10.3969/j.issn.1006-1959.2022.19.001
文献标志码:
A
摘要:
目的 运用生物信息学分析方法，分析长链非编码RNA-Hexokinase 2（HK2）在细胞内的调控靶基因及信号通路，并对其进行生物学功能注释，阐述lncRNA- HK2在细胞内的功能作用。方法 在miRDB数据库和Starbase3.0数据库中预测与lncRNA-HK2有miRNA-lncRNA互作关系的miRNA，将结果取交集。在Starbase3.0数据库中预测miRNAs靶基因，并进行GO功能注释和Pathway通路富集分析。结果 在miRDB数据库预测到50个miRNAs与lncRNA-HK2相作用，Starbase3.0数据库预测到14个miRNAs与之相作用，取2个数据库的交集得到hsa-miR-374a-5p、hsa-miR-374b-5p 2个miRNAs；GO功能注释显示hsa-miR-374a-5p的靶基因主要调控蛋白磷酸化、肿瘤发生通路、转录调控、DNA损伤反应、脂肪细胞分化及胚胎发育等基因及信号通路； hsa-miR-374b-5p作用的靶基因主要调控基因表达，调控序列特异性DNA结合转录因子的活性、翻译调控、细胞对于胰岛素的反应、细胞粘附及细胞周期等生物学过程，信号通路富集结果主要分布在TNF 信号通路、RAS信号通路、人乳头状瘤病毒感染、转录错误调控及多种肿瘤信号通路等方面。结论 长链非编码RNA HK2在细胞内的重要的调控作用，具体与蛋白磷酸化、肿瘤发生通路、转录调控、DNA损伤反应、翻译以及细胞周期等多条信号通路密切相关，其主要通过上述作用通路调控生理和病理进程。
Abstract:
Objective To analyze the regulatory target genes and signaling pathways of lncRNA-HK2 in cells by bioinformatics analysis, and annotate its biological functions, so as to explain the functions of lncRNA-HK2 in cells.Methods The miRNAs interacting with lncRNA-HK2 were predicted in miRDB database and Starbase3.0 database, and the results were intersected. Target genes of miRNAs were predicted in the Starbase 3.0 database, and GO functional annotation and Pathway enrichment analysis were performed.Results A total of 50 miRNAs were predicted to interact with lncRNA-HK2 in miRDB database, and 14 miRNAs were predicted to interact with lncRNA-HK2 in Starbase3.0 database. A total of 2 miRNAs from the intersection of the two databases were selected for further analysis. GO functional annotation showed that the target genes of hsa-miR-374a-5p mainly regulated protein phosphorylation, tumorigenesis pathway, transcriptional regulation, DNA damage response, adipocyte differentiation and embryonic development. The target genes of hsa-miR-374b-5p mainly regulate gene expression, regulate the activity of sequence-specific DNA binding transcription factors, translation regulation, cell response to insulin, cell adhesion, cell cycle and other biological processes. The results of signal pathway enrichment are mainly distributed in TNF signaling pathway, RAS signaling pathway, human papillomavirus infection, transcriptional error regulation and various tumor signaling pathways.Conclusion The important regulatory role of long non-coding RNA HK2 in cells is closely related to multiple signaling pathways such as protein phosphorylation, tumorigenesis pathways, transcriptional regulation, DNA damage response, translation, and cell cycle. It mainly regulates physiological and pathological processes through the above-mentioned pathways.

参考文献/References:

[1]Zhang Y,Zhao L,Yang S,et al.CircCDKN2B-AS1 interacts with IMP3 to stabilize hexokinase 2 mRNA and facilitate cervical squamous cell carcinoma aerobic glycolysis progression[J].J Exp Clin Cancer Res,2020,39(1):281.[2]Wen JF,Jiang YQ,Li C,et al.LncRNA-SARCC sensitizes osteosarcoma to cisplatin through the miR-143-mediated glycolysis inhibition by targeting Hexokinase 2[J].Cancer Biomark,2020,28(2):231-246.[3]Chen J,Yu Y,Li H,et al.Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer[J].Mol Cancer,2019,18(1):33.[4]Gao Y,Wang Z,Tong J,et al.LncRNA loc285194 inhibits tumor growth of laryngeal squamous cell carcinoma cells by downregulating hexokinase 2[J].Exp Ther Med,2019,18(4):2378-2384.[5]Gao SJ,Ren SN,Liu YT,et al.Targeting EGFR sensitizes 5-Fu-resistant colon cancer cells through modification of the lncRNA-FGD5-AS1-miR-330-3p-Hexokinase 2 axis[J].Mol Ther Oncolytics,2021,23:14-25.[6]Chen J,Yu Y,Li H,et al.Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer[J].Mol Cancer,2019,18(1):33.[7]Zhang X,Cui Y,Ding X,et al.Analysis of mRNA lncRNA and mRNA lncRNA-pathway co expression networks based on WGCNA in developing pediatric sepsis[J].Bioengineered,2021,12(1):1457-1470.[8]Song H,Li D,Wang X,et al.HNF4A-AS1/hnRNPU/CTCF axis as a therapeutic target for aerobic glycolysis and neuroblastoma progression[J].J Hematol Oncol,2020,13(1):24.[9]Zhang J,Chen G,Gao Y,et al.HOTAIR/miR-125 axis-mediated Hexokinase 2 expression promotes chemoresistance in human glioblastoma[J].J Cell Mol Med,2020,24(10):5707-5717.[10]Fan JL,Zhu TT,Xue ZY,et al.lncRNA-XIST protects the hypoxia-induced cardiomyocyte injury through regulating the miR-125b-hexokianse 2 axis[J].In Vitro Cell Dev Biol Anim,2020,56(4):349-357.[11]Zhang Y,Liu Y,Xu X.Knockdown of LncRNA-UCA1 suppresses chemoresistance of pediatric AML by inhibiting glycolysis through the microRNA-125a/hexokinase 2 pathway[J].J Cell Biochem,2018,119(7):6296-6308.[12]Chen C,Wei M,Wang C,et al.Long noncoding RNA KCNQ1OT1 promotes colorectal carcinogenesis by enhancing aerobic glycolysis via hexokinase-2[J].Aging (Albany NY),2020,12(12):11685-11697. [13]Wang L,Huang Q,Lin Q,et al.Knockdown of long non-coding RNA small nucleolar RNA host gene 9 or hexokinase 2 both suppress endometrial cancer cell proliferation and glycolysis[J].J Obstet Gynaecol Res,2021,47(6):2196-2203.[14]Yu P,Wilhelm K,Dubrac A,et al.FGF-dependent metabolic control of vascular development[J].Nature,2017,545:224-228.[15]Yin X,Choudhury M,Kang JH,et al.Hexokinase 2 couples glycolysis with the profibrotic actions of TGF-beta[J].Signal,2019,12:eaax4067. [16]Podar K,Anderson KC.A therapeutic role for targeting c-akt/Hif-1-dependent signaling pathways[J].Cell Cycle,2010,9:1722-1728.[17]Corona JC,Gimenez-Cassina A,Lim F,et al.Hexokinase II gene transfer protects against neurodegeneration in the rotenone and MPTP mouse models of Parkinson’s disease[J].J Neurosci Res,2010,88:1943-1950.[18]Li C,Wang P,Du J,et al.LncRNA RAD51-AS1/miR-29b/c-3p/NDRG2 crosstalk repressed proliferation, invasion and glycolysis of colorectal cancer[J].IUBMB Life,2021,73(1):286-298.

相似文献/References:

[1]侯梦月,黄金智,张玲莉.SIRT2及其与子宫内膜癌关系的研究进展[J].医学信息,2019,32(05):31.[doi:10.3969/j.issn.1006-1959.2019.05.012]
　HOU Meng-yue,HUANG Jin-zhi,ZHANG Ling-li.Progress in the Study of SIRT2 and its Relationship with Endometrial Cancer[J].Journal of Medical Information,2019,32(19):31.[doi:10.3969/j.issn.1006-1959.2019.05.012]
[2]崔方强.足细胞上皮间质转分化研究[J].医学信息,2019,32(22):21.[doi:10.3969/j.issn.1006-1959.2019.22.008]
　CUI Fang-qiang.Epithelial Transdifferentiation of Podocytes[J].Journal of Medical Information,2019,32(19):21.[doi:10.3969/j.issn.1006-1959.2019.22.008]
[3]张伟斌,荆国杰.胶质母细胞瘤信号通路的研究[J].医学信息,2019,32(23):33.[doi:10.3969/j.issn.1006-1959.2019.23.009]
　ZHANG Wei-bin,JING Guo-jie.Study on Glioblastoma Signaling Pathway[J].Journal of Medical Information,2019,32(19):33.[doi:10.3969/j.issn.1006-1959.2019.23.009]
[4]王东梅,张展英.小细胞肺癌中信号通路及蛋白相互作用网络分析[J].医学信息,2020,33(01):81.[doi:10.3969/j.issn.1006-1959.2020.01.025]
　WANG Dong-mei,ZHANG Zhan-ying.Analysis of Signal Pathways and Protein Interaction Networks in Small Cell Lung Cancer[J].Journal of Medical Information,2020,33(19):81.[doi:10.3969/j.issn.1006-1959.2020.01.025]
[5]焦 乐.PEBP4蛋白在复发性脑胶质瘤患者中的表达情况及临床意义分析[J].医学信息,2020,33(08):131.[doi:10.3969/j.issn.1006-1959.2020.08.042]
　JIAO Le.Expression and Clinical Significance of PEBP4 Protein in Patients with Recurrent Glioma[J].Journal of Medical Information,2020,33(19):131.[doi:10.3969/j.issn.1006-1959.2020.08.042]
[6]朱明明,李占全,崔 森.MMP-9信号通路调控乳腺癌发展的机制[J].医学信息,2020,33(13):22.[doi:10.3969/j.issn.1006-1959.2020.13.007]
　ZHU Ming-ming,LI Zhan-quan,CUI Sen.Mechanism of MMP-9 Signaling Pathway Regulating Breast Cancer Development[J].Journal of Medical Information,2020,33(19):22.[doi:10.3969/j.issn.1006-1959.2020.13.007]
[7]廖 伟,蔡崇明,陈金明,等.miR-200b-Notch信号通路对诱导性多能干细胞向神经干细胞分化的调控作用[J].医学信息,2020,33(16):65.[doi:10.3969/j.issn.1006-1959.2020.16.020]
　LIAO Wei,CAI Chong-ming,CHEN Jin-ming,et al.Regulation of miR-200b-Notch Signaling Pathway on the Differentiation of Induced Pluripotent Stem Cells into Neural Stem Cells[J].Journal of Medical Information,2020,33(19):65.[doi:10.3969/j.issn.1006-1959.2020.16.020]
[8]文先丽,施会敏,张爱青.长链非编码RNA相关信号通路参与肾脏纤维化的研究[J].医学信息,2020,33(21):26.[doi:10.3969/j.issn.1006-1959.2020.21.009]
　WEN Xian-li,SHI Hui-min,ZHANG Ai-qing.Involvement of Long-chain Non-coding RNA Related Signaling Pathways in Renal Fibrosis[J].Journal of Medical Information,2020,33(19):26.[doi:10.3969/j.issn.1006-1959.2020.21.009]
[9]阳华妹.程序性死亡通路在胆红素脑病机制中的研究[J].医学信息,2021,34(10):43.[doi:10.3969/j.issn.1006-1959.2021.10.012]
　YANG Hua-mei.The Study of Programmed Death Pathway in the Mechanism of Bilirubin Encephalopathy[J].Journal of Medical Information,2021,34(19):43.[doi:10.3969/j.issn.1006-1959.2021.10.012]
[10]吴亚娟,赵 楠,钱科佳,等.基于网络生物信息学筛选精神分裂症关键基因[J].医学信息,2021,34(16):1.[doi:10.3969/j.issn.1006-1959.2021.16.001]
　WU Ya-juan,ZHAO Nan,QIAN Ke-jia,et al.Screening Key Genes of Schizophrenia Based on Network Bioinformatics[J].Journal of Medical Information,2021,34(19):1.[doi:10.3969/j.issn.1006-1959.2021.16.001]

更新日期/Last Update: 1900-01-01