[1]王雨娜,吴冬桂,赵 贝.基于数据挖掘AGR2在胰腺癌中的表达及预后潜力[J].医学信息,2022,35(22):9-16.[doi:10.3969/j.issn.1006-1959.2022.22.002]
　WANG Yu-na,WU Dong-gui,ZHAO Bei.Expression and Prognostic Potential of AGR2 in Pancreatic Cancer Based on Data Mining[J].Journal of Medical Information,2022,35(22):9-16.[doi:10.3969/j.issn.1006-1959.2022.22.002]
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基于数据挖掘AGR2在胰腺癌中的表达及预后潜力()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年22期
页码:
9-16
栏目:
生物信息学
出版日期:
2022-11-15

文章信息/Info

Title:
Expression and Prognostic Potential of AGR2 in Pancreatic Cancer Based on Data Mining
文章编号:
1006-1959（2022）22-0009-08
作者:
王雨娜; 吴冬桂; 赵 贝
（1.大理大学基础医学院，云南 大理 671000；2.珠海市人民医院外科，广东 珠海 519000）
Author(s):
WANG Yu-na; WU Dong-gui; ZHAO Bei
(1.School of Basic Medical Sciences,Dali University,Dali 671000,Yunnan,China;2.Department of Surgery,Zhuhai People’s Hospital,Zhuhai 519000,Guangdong,China)
关键词:
胰腺癌; 前梯度蛋白; 差异表达基因; 共表达蛋白组
Keywords:
Pancreatic cancer; Anterior gradient; Differentially expressed genes; Co-expression protein group
分类号:
R735.9
DOI:
10.3969/j.issn.1006-1959.2022.22.002
文献标志码:
A
摘要:
目的 探究前梯度蛋白2（AGR2）和非肿瘤胰腺组织与胰腺癌之间的关系，汇总AGR2在胰腺癌组织的表达情况，为后续的肿瘤风险评估和治疗提供有力的依据。方法 利用GEO数据库分析AGR2基因表达及染色体定位，利用Oncomine数据库分析AGR2 mRNA在胰腺癌芯片中的表达状态，基于GEPIA2数据库挖掘出AGR2与胰腺癌病理分期及预后的关系，借助String数据库分析AGR2在癌症基因表达的关键靶蛋白，借助GeneMANIA分析AGR2基因在胰腺癌发展过程中可能的调控通路，通过UALCAN数据库在多种样本综合分析条件下评估AGR2甲基化对胰腺癌患者生存预后的影响。结果 AGR2基因在胰腺癌癌组织中的表达水平高于正常组织，差异有统计学意义（P＜0.05）；不同病理分期胰腺癌患者AGR2基因表达比较，差异有统计学意义（P＜0.05）；AGR2共表达基因分析显示，S100P、CDC25B、C-K-RAS、HER3 等基因与 AGR2 基因表达呈正相关；AGR2基因主要负责蛋白质泛素化的正向调控、Wnt通路的调控以及共表达蛋白组靶向。结论 AGR2在胰腺癌组织中呈高表达，其表达水平与胰腺癌的预后有关，有望成为胰腺癌预后评估和治疗的有效靶点。
Abstract:
Objective To explore the relationship between anterior gradient 2 (AGR2) and non-tumor pancreatic tissue and pancreatic cancer, and to summarize the expression of pre-gradient protein AGR2 and pancreatic cancer tissue, so as to provide a strong therapeutic basis for subsequent tumor risk assessment and treatment.Methods The GEO database was used to analyze the expression and chromosomal localization of AGR2 gene. The Oncomine database was used to analyze the expression status of AGR2 mRNA in pancreatic cancer microarray. The relationship between AGR2 and pathological stage and prognosis of pancreatic cancer was explored based on the GEPIA2 database. The String database was used to analyze the key target proteins of AGR2 in cancer gene expression. The possible regulatory pathways of AGR2 gene in the development of pancreatic cancer were analyzed by So GeneMANIA. The effect of AGR2 methylation on the survival prognosis of patients with pancreatic cancer was evaluated by the UALCAN database under a comprehensive analysis of multiple samples.Results The expression level of AGR2 gene in pancreatic cancer was higher than that in normal tissues (P<0.05). The expression of AGR2 gene in pancreatic cancer patients with different pathological stages was statistically significant (P<0.05). AGR2 co-expression gene analysis showed that S100P, CDC25B, C-K-RAS and HER3 were positively correlated with AGR2 gene expression. AGR2 gene is mainly responsible for the positive regulation of protein ubiquitination, the regulation of Wnt pathway and the targeting of co-expressed proteome.Conclusion AGR2 is highly expressed in pancreatic cancer tissues, and its expression level is related to the prognosis of pancreatic cancer. It is expected to become an effective target for prognosis evaluation and treatment of pancreatic cancer.

参考文献/References:

[1]Zhu H,Wei M,Xu J,et al.PARP inhibitors in pancreatic cancer: molecular mechanisms and clinical applications[J].Mol Cancer,2020,19(1):49. [2]Moidu NA,A Rahman NS,Syafruddin SE,et al.Secretion of pro-oncogenic AGR2 protein in cancer[J].Heliyon.2020,6(9):e05000.[3]Ye Z,Zhuo Q,Hu QX,et al.FBW7-NRA41-SCD1 axis synchronously regulates apoptosis and ferroptosis in pancreatic cancer cells[J].Redox Biol,2021,38:101807.[4]Singh HM,Bailey P,Hübschmann D,et al.Poly(ADP-ribose) polymerase inhibition in pancreatic cancer[J].Genes Chromosomes Cancer,2021,60(5):373-384. [5]Roger E,Gout J,Arnold F,et al.Maintenance Therapy for ATM-Deficient Pancreatic Cancer by Multiple DNA Damage Response Interferences after Platinum-Based Chemotherapy[J].Cells,2020,9(9):2110. [6]Li K,Du Y,Li L,et al.Bioinformatics Approaches for Anti-cancer Drug Discovery[J].Curr Drug Targets,2020,21(1):3-17. [7]Pittard WS,Villaveces CK,Li S.A Bioinformatics Primer to Data Science, with Examples for Metabolomics[J].Methods Mol Biol,2020,2104:245-263.[8]Bai J,Bandla C,Guo J,et al.BioContainers Registry: Searching Bioinformatics and Proteomics Tools, Packages, and Containers[J].J Proteome Res,2021,20(4):2056-2061. [9]Zheng HJ,Hu XT.Multiscale network-based approaches in bioinformatics and biomedicine[J].Methods,2020,173:1-2. [10]Yu S,Zhang C,Xie KP.Therapeutic resistance of pancreatic cancer: Roadmap to its reversal[J].Biochim Biophys Acta Rev Cancer,2021,1875(1):188461. [11]Hu Y,Guo M.Synthetic lethality strategies: Beyond BRCA1/2 mutations in pancreatic cancer[J].Cancer Sci,2020,111(9):3111-3121.[12]Rawat M,Kadian K,Gupta Y,et al.MicroRNA in Pancreatic Cancer: From Biology to Therapeutic Potential[J].Genes (Basel),2019,10(10):752.[13]Tian S,Chu Y,Hu J,et al.Tumour-associated neutrophils secrete AGR2 to promote colorectal cancer metastasis via its receptor CD98hc-xCT[J].Gut,2022:gutjnl-2021-325137. [14]Zhang Z,Li H,Deng Y,et al.AGR2-Dependent Nuclear Import of RNA Polymerase II Constitutes a Specific Target of Pancreatic Ductal Adenocarcinoma in the Context of Wild-Type p53[J].Gastroenterology,2021,161(5):1601-1614.[15]Jach D,Cheng Y,Prica F,et al.T. From development to cancer-an ever-increasing role of AGR2[J].Am J Cancer Res,2021,11(11):5249-5262.[16]Fessart D,de Barbeyrac C,Boutin I,et al.Extracellular AGR2 triggers lung tumour cell proliferation through repression of p21CIP1[J].Biochim Biophys Acta Mol Cell Resm,2021,1868(3):118920. [17]Qu X,Yeung C,Coleman I,et al.Comparison of four next generation sequencing platforms for fusion detection: Oncomine by ThermoFisher, AmpliSeq by illumina, FusionPlex by ArcherDX, and QIAseq by QIAGEN[J].Cancer Genet,2020,243:11-18. [18]Tian Z,He W,Tang J,et al.Identification of Important Modules and Biomarkers in Breast Cancer Based on WGCNA[J].Onco Targets Ther,2020,13:6805-6817. [19]Tang Z,Kang B,Li C,et al.GEPIA2: an enhanced web server for large-scale expression profiling and interactive analysis[J].Nucleic Acids Res,2019,47(W1):W556-W560. [20]Szklarczyk D,Gable AL,Nastou KC,et al.The STRING database in 2021:customizable protein-protein networks, and functional characterization of user-uploaded gene/measurement sets[J].Nucleic Acids Res,2021,49(D1):D605-D612.[21]陈展,崔强,齐闯,等.基于数据挖掘分析PEG3基因在肾透明细胞癌中的表达及预后意义[J].现代肿瘤医学,2018(4):548-553.[22]梅雯,孙如丽,李云,等.基于数据挖掘分析TOP2A在胰腺癌中的表达及预后意义[J].大理大学学报,2020,5(10):40-47.[23]Huang X,Tang T,Zhang G,et al.Identification of tumor antigens and immune subtypes of cholangiocarcinoma for mRNA vaccine development[J].Mol Cancer,2021,20(1):50.

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更新日期/Last Update: 1900-01-01