[1]邹震海,程 琪,李 中,等.基于生物信息学筛选膀胱癌预后相关的关键miRNAs并验证[J].医学信息,2022,35(22):1-8.[doi:10.3969/j.issn.1006-1959.2022.22.001]
　ZOU Zhen-hai,CHENG Qi,LI Zhong,et al.Screening and Verification Key miRNAs Related to Prognosis of Bladder Cancerbased on Bioinformatics[J].Journal of Medical Information,2022,35(22):1-8.[doi:10.3969/j.issn.1006-1959.2022.22.001]
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基于生物信息学筛选膀胱癌预后相关的关键miRNAs并验证()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
35卷
期数:
2022年22期
页码:
1-8
栏目:
生物信息学
出版日期:
2022-11-15

文章信息/Info

Title:
Screening and Verification Key miRNAs Related to Prognosis of Bladder Cancerbased on Bioinformatics
文章编号:
1006-1959（2022）22-0001-08
作者:
邹震海; 程 琪; 李 中; 等
（蚌埠医学院第一附属医院泌尿外科，安徽 蚌埠 233004）
Author(s):
ZOU Zhen-hai; CHENG Qi; LI Zhong; et al.
(Department of Urology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,Anhui,China)
关键词:
膀胱肿瘤; miRNA; 生物信息学; miR-944
Keywords:
Bladder cancer; miRNA; Bioinformatics; miR-944
分类号:
R737.14
DOI:
10.3969/j.issn.1006-1959.2022.22.001
文献标志码:
A
摘要:
目的 筛选并分析与膀胱癌预后相关的关键microRNAs（miRNAs）及其靶基因并进行实验验证。方法 从TCGA数据库中下载膀胱癌及正常组织的miRNAs数据及相应的预后信息；利用edgeR包、survival包获取既存在表达差异又有OS临床预后意义的miRNAs分子；将优选的miRNAs进行临床相关性分析，得到关键miRNAs。利用3大常用miRNA预测靶基因的数据库（miRDB、miRWalk、TargetScan）预测关键miRNAs的靶基因，采用DAVID数据库对靶基因进行GO和KEGG富集分析。qRT-PCR检测关键miRNA在膀胱癌细胞中的表达水平；通过转染过表达T24细胞中miR-1307-5p和miR-944，检测细胞增殖、迁移、侵袭和凋亡能力的变化。结果 从TCGA数据库中共下载418例膀胱癌及19例正常对照的miRNA数据，共筛选出293个差异miRNAs，高表达224个和低表达69个。通过单因素分析优选出与患者预后显著相关的9个miRNAs分子，进一步分析出与患者临床病理信息密切相关的2个miRNA，分别为miR-1307-5p和miR-944。GO和KEGG分析显示，这些miRNAs的靶基因主要与细胞内的细胞质、细胞器以及金属阳离子的结合有关，并且参与了多种与致癌相关的信号通路。qRT-PCR分析显示，miR-944在膀胱癌细胞中的表达下调（P＜0.05），miR-1307-5p的表达水平无明显变化（P＞0.05）；过表达miR-944可抑制膀胱癌细胞增殖、迁移和侵袭，促进细胞凋亡，但过表达miR-1307-5p后膀胱癌细胞的增殖、迁移、侵袭和凋亡无明显变化。结论 基于生物信息学分析获得具有重要临床意义的2个关键miRNAs分子，分别是miR-1307-5p和miR-944，其中miR-944可以抑制膀胱癌细胞的生物行为，可能作为膀胱癌潜在的治疗靶点。
Abstract:
Objective To screen and analyze the key microRNAs (miRNAs) and their target genes related to the prognosis of bladder cancer, and carry out experimental verification.Methods The miRNAs data of BLCA and normal tissues and the corresponding prognosis information were downloaded from TCGA database. Use edgeR package and survival package to obtain miRNAs molecules with both expression differences and clinical prognostic significance of OS; clinical correlation analysis of the preferred miRNAs was carried out to obtain the key miRNAs. Three commonly used databases (miRDB, miRWalk and TargetScan) were used to predict the target genes of key miRNA, and DAVID database was used for GO and KEGG enrichment analysis of the target genes. The expression level of key miRNA in bladder cancer cells was detected by qRT-PCR. By transfecting the expression of miR-1307-5p and miR-944 in the overexpressed T24 cells, the changes of cell proliferation, migration, invasion and apoptosis were detected.Results A total of 418 cases of bladder cancer and 19 normal controls were downloaded from TCGA database, and 293 differential miRNAs were screened, of which 224 were highly expressed and 69 were low expressed. Through single factor analysis, nine miRNAs molecules which were significantly related to the prognosis of patients were selected, and two miRNAs which were closely related to the clinicopathological information of patients were further analyzed, namely miR-1307-5p and miR-944. GO and KEGG analysis showed that the target genes of these miRNAs were mainly related to the combination of cytoplasm, organelles and metal cations in cells, and participated in a variety of signal pathways related to carcinogenesis. The results of qRT-PCR showed that the expression of miR-944 in bladder cancer cells was significantly down-regulated (P<0.05), but the expression level of miR-1307-5p had no significant change (P>0.05). Over-expression of miR-944 could inhibit the proliferation, migration and invasion of bladder cancer cells and promote apoptosis, but over-expression of miR-1307-5p had no obvious changes in the proliferation, migration, invasion and apoptosis of bladder cancer cells.Conclusion Based on bioinformatics analysis, two key miRNAs molecules with important clinical significance are obtained, namely miR-1307-5p and miR-944. miR-944 can inhibit the biological behavior of bladder cancer cells, and may be a potential therapeutic target for bladder cancer.

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更新日期/Last Update: 1900-01-01