[1]李 坤,韩 娜,凡志祥,等.DLGAP5作为肾透明细胞癌的诊断标志物及其与CD8+T细胞的相关性[J].医学信息,2023,36(07):1-8.[doi:10.3969/j.issn.1006-1959.2023.07.001]
　LI Kun,HAN Na,FAN Zhi-xiang,et al.DLGAP5 as a Diagnostic Marker of Renal Clear Cell Carcinoma and its Correlation with CD8+ T Cells[J].Journal of Medical Information,2023,36(07):1-8.[doi:10.3969/j.issn.1006-1959.2023.07.001]
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DLGAP5作为肾透明细胞癌的诊断标志物及其与CD8+T细胞的相关性()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年07期
页码:
1-8
栏目:
生物信息学
出版日期:
2023-04-01

文章信息/Info

Title:
DLGAP5 as a Diagnostic Marker of Renal Clear Cell Carcinoma and its Correlation with CD8+ T Cells
文章编号:
1006-1959（2023）07-0001-08
作者:
李 坤; 韩 娜; 凡志祥; 等
（郑州大学第二附属医院肿瘤科，河南 郑州 450014）
Author(s):
LI Kun; HAN Na; FAN Zhi-xiang; et al.
(Department of Oncology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,Henan,China)
关键词:
DLGAP5; CD8+T细胞; KIRC; TCGA数据库; 预后
Keywords:
DLGAP5; CD8+ T cells; KIRC; TCGA database; Prognosis
分类号:
R737.11
DOI:
10.3969/j.issn.1006-1959.2023.07.001
文献标志码:
A
摘要:
目的 探讨Discs大同源物关联蛋白5（DLGAP5）表达在肾透明细胞癌（KIRC）中的作用。方法 基于癌症基因组图谱（TCGA）分析KIRC中DLGAP5的表达情况与临床参数之间的相关性。应用比例风险（Cox）回归模型和Kaplan-Meier（K-M）分析评估了DLGAP5在KIRC患者中的预后价值。CIBERSORT分析DLGAP5和CD8+T细胞之间的相关性，基因集富集分析（GSEA）方法筛选和DLGAP5表达相关的生物学途径。结果 与正常肾脏组织比较，KIRC中DLGAP5的表达呈显著上调。其高表达情况与肿瘤组织的分化程度、病理分期、复发和死亡均显著相关。K-M分析结果表明，DLGAP5高表达与KIRC患者的不良总生存期（OS）和无进展生存期（PFS）相关，且DLGAP5是OS和PFS的独立预后因子。免疫微环境分析表明，DLGAP5的表达量与多种肿瘤免疫细胞相关，尤其是CD8+T细胞。DLGAP5表达在“细胞周期”“泛素介导的蛋白水解”“蛋白酶体通路”和“T细胞受体信号通路”上显著富集。药敏分析结果显示，高危组患者对阿昔替尼、舒尼替尼更敏感。结论 DLGAP5是KIRC的一种潜在生物标志物，与KIRC的诊断与预后显著相关，这将为临床的诊断与治疗提供新的研究方向。
Abstract:
Objective To investigate the role of discs large homolog associated protein 5 (DLGAP5) expression in renal clear cell carcinoma (KIRC).Methods The correlation between the expression of DLGAP5 in KIRC and clinical parameters was analyzed based on The Cancer Genome Atlas (TCGA). The prognostic value of DLGAP5 in KIRC patients was evaluated by proportional risk (Cox) regression model and Kaplan-Meier (K-M) analysis. CIBERSORT was used to analyze the correlation between DLGAP5 and CD8+ T cells. Gene set enrichment analysis (GSEA) was used to screen the biological pathways related to DLGAP5 expression.Results Compared with normal kidney tissues, the expression of DLGAP5 in KIRC was significantly up-regulated. Its high expression was significantly correlated with tumor differentiation, pathological stage, recurrence and death. K-M analysis showed that high expression of DLGAP5 was associated with poor overall survival (OS) and progression-free survival (PFS) in KIRC patients, and DLGAP5 was an independent prognostic factor for OS and PFS. Immune microenvironment analysis showed that the expression of DLGAP5 was associated with a variety of tumor immune cells, especially CD8+ T cells. DLGAP5 expression was significantly enriched in “cell cycle” “ubiquitin-mediated proteolysis” “ proteasome pathway”and “T cell receptor signaling pathway”. The results of drug sensitivity analysis showed that patients in the high-risk group were more sensitive to axitinib and sunitinib.Conclusion DLGAP5 is a potential biomarker of KIRC, which is significantly related to the diagnosis and prognosis of KIRC, and will provide a new research direction for clinical diagnosis and treatment.

参考文献/References:

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更新日期/Last Update: 1900-01-01