[1]李 南,曾凡业,仝梦婷,等.应用流式细胞术检测胃癌移植瘤小鼠脾脏中MDSC及T淋巴细胞亚群的变化[J].医学信息,2021,34(16):77-80.[doi:10.3969/j.issn.1006-1959.2021.16.021]
　LI Nan,ZENG Fan-ye,TONG Meng-ting,et al.Application of Flow Cytometry to Detect the Changes of MDSC and T Lymphocyte Subsets in the Spleen of Mice with Gastric Cancer Transplantation[J].Medical Information,2021,34(16):77-80.[doi:10.3969/j.issn.1006-1959.2021.16.021]
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应用流式细胞术检测胃癌移植瘤小鼠脾脏中MDSC及T淋巴细胞亚群的变化()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年16期
页码:
77-80
栏目:
论著
出版日期:
2021-08-15

文章信息/Info

Title:
Application of Flow Cytometry to Detect the Changes of MDSC and T Lymphocyte Subsets in the Spleen of Mice with Gastric Cancer Transplantation
文章编号:
1006-1959（2021）16-0077-04
作者:
李 南; 曾凡业; 仝梦婷; 等
（新疆医科大学第四附属医院肿瘤二科，新疆 乌鲁木齐 830000）
Author(s):
LI Nan; ZENG Fan-ye; TONG Meng-ting; et al.
(Second Department of Oncology,the Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,Xinjiang,China)
关键词:
骨髓源性抑制细胞; T淋巴细胞亚群; 胃癌; CD4+T细胞; CD8+T细胞
Keywords:
Bone marrow-derived suppressor cells; T lymphocyte subsets; Gastric cancer; CD4+T cells; CD8+T cells
分类号:
R392
DOI:
10.3969/j.issn.1006-1959.2021.16.021
文献标志码:
A
摘要:
目的 观察胃荷瘤小鼠脾脏中髓源性抑制细胞（MDSC)、调节性T细胞(Treg)和传统T淋巴细胞群的变化。方法 随机将30只C57BL/6J小鼠分成荷瘤组和正常组，荷瘤组小鼠模型右侧背部皮下注射小鼠前胃癌细胞，正常组注射等量生理盐水，待肿瘤形成后，采用多色流式分析检测两组小鼠脾脏中MDSC、Treg以及CD4+和CD8+T细胞数量和相应比例，膜联蛋白-V（Annexin-V）染色检测CD4+和CD8+T细胞凋亡变化，5-溴脱氧尿嘧啶核苷（BrdU）染色检测CD4+和CD8+T细胞增殖变化。结果 荷瘤组小鼠脾脏中MDSC和Treg占总脾脏细胞中的数量和比例高于正常组，CD4+和CD8+T细胞数量和比例低于正常组，差异有统计学意义（P＜0.05）。荷瘤组小鼠脾脏中CD4+和CD8+T细胞增殖低于正常组，CD8+T细胞凋亡高于正常组，差异有统计学意义（P＜0.05）。结论 MDSC和Treg细胞可积聚在胃荷瘤小鼠脾脏中，且其能抑制CD4+和CD8+T细胞增殖和促进CD8+T细胞凋亡。
Abstract:
Objective To observe the changes of myeloid-derived suppressor cells (MDSC), regulatory T cells (Treg) and traditional T lymphocyte populations in the spleen of mice bearing gastric tumors.Methods A total of 30 C57BL/6J mice were randomly divided into tumor-bearing group and normal group. The right back of the tumor-bearing group was injected with mouse forestomach carcinona cell, and the normal group was injected with the same amount of normal saline. After the tumor formed, polychromatic flow was used. Formula analysis was used to detect the number and corresponding proportions of MDSC, Treg, CD4+ and CD8+ T cells in the spleens of the two groups of mice. Annexin-V (Annexin-V) staining was used to detect the changes of CD4+ and CD8+ T cell apoptosis, 5-bromodeoxy Uracil (BrdU) staining was used to detect the proliferation of CD4+ and CD8+ T cells.Results The number and proportion of MDSC and Treg in the total spleen cells in the spleen of the tumor-bearing mice were higher than that of the normal group, and the number and proportion of CD4+ and CD8+ T cells were lower than the normal group,the difference was statistically significant(P<0.05).The proliferation of CD4+ and CD8+ T cells in the spleen of mice in the tumor-bearing group was lower than that in the normal group, and the apoptosis of CD8+ T cells was higher than that in the normal group,the difference was statistically significant (P<0.05).Conclusion MDSC and Treg cells can accumulate in the spleen of gastric tumor-bearing mice, and they can inhibit the proliferation of CD4+ and CD8+ T cells and promote the apoptosis of CD8+ T cells.

参考文献/References:

[1]Machlowska J,Baj J,Sitarz M,et al.Gastric Cancer: Epidemiology, Risk Factors, Classification, Genomic Characteristics and Treatment Strategies[J].Int J Mol Sci,2020,21(11):4012. [2]Law A,Valdes-Mora F,Gallego-Ortega D.Myeloid-Derived Suppressor Cells as a Therapeutic Target for Cancer[J].Cells,2020,9(3):561. [3]Yang L,Wang B,Qin J,et al. Blockade of CCR5-mediated myeloid derived suppressor cell accumulation enhances anti-PD1 efficacy in gastric cancer[J]. Immunopharmacol Immunotoxicol,2018,40(1):91-97. [4]陈思文,王翎,苏楠,等.老年荷瘤小鼠髓源性抑制细胞亚群检测及功能研究[J].中华老年医学杂志,2016,35(6):651-655. [5]Sabine H,Loh J,Cristina R,et al.Synergy between CD8 T Cells and Th1 or Th2 Polarised CD4 T Cells for Adoptive Immunotherapy of Brain Tumours[J].PLoS One,2013,8(5):e63933. [6]Diaz-Montero CM,Finke J,Montero AJ.Myeloid-derived suppressor cells in cancer: therapeutic, predictive, and prognostic implications[J].Semin Oncol,2014,41(2):174-184. [7]Lindau D,Gielen P,Kroesen M,et al.The immunosuppressive tumour network: myeloid-derived suppressor cells, regulatory T cells and natural killer T cells[J].Immunology,2013,138(2):105-115. [8]Onyilagha C,Kuriakose S,Ikeogu N,et al.Myeloid-Derived Suppressor Cells Contribute to Susceptibility to Trypanosoma congolense Infection by Suppressing CD4+ T Cell Proliferation and IFN-γ Production[J].J Immunol,2018,201(2):507-515. [9]Ornstein MC,Diaz-Montero CM,Rayman P,et al.Myeloid-derived suppressors cells (MDSC) correlate with clinicopathologic factors and pathologic complete response (pCR) in patients with urothelial carcinoma (UC) undergoing cystectomy[J].Urol Oncol,2018,36(9):405-412. [10]Ibánez-Vea M,Zuazo M,Gato M,et al.Myeloid-Derived Suppressor Cells in the Tumor Microenvironment: Current Knowledge and Future Perspectives[J].Arch Immunol Ther Exp (Warsz),2018,66(2):113-123.

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更新日期/Last Update: 1900-01-01