[1]蒋志明.MAP4/Mps1信号通路介导miR-21对心肌成纤维化细胞的影响[J].医学信息,2023,36(09):74-77.[doi:10.3969/j.issn.1006-1959.2023.09.013]
 JIANG Zhi-ming.Effect of MAP4/Mps1 Signaling Pathway Mediates miR-21 on Myocardial Fibroblast Cells[J].Journal of Medical Information,2023,36(09):74-77.[doi:10.3969/j.issn.1006-1959.2023.09.013]
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MAP4/Mps1信号通路介导miR-21对心肌成纤维化细胞的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年09期
页码:
74-77
栏目:
论著
出版日期:
2023-05-01

文章信息/Info

Title:
Effect of MAP4/Mps1 Signaling Pathway Mediates miR-21 on Myocardial Fibroblast Cells
文章编号:
1006-1959(2023)09-0074-04
作者:
蒋志明
(长沙市第四医院心血管内一科,湖南 长沙 410006)
Author(s):
JIANG Zhi-ming
(Department of Cardiology,the Fourth Hospital of Changsha,Changsha 410006,Hunan,China)
关键词:
心肌纤维化miR-21MAP4MPS1α-SMAColⅢCol Ⅰ
Keywords:
Myocardial fibrosismiR-21MAP4MPS1α-SMACol ⅢCol Ⅰ
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2023.09.013
文献标志码:
A
摘要:
目的 探讨微管相关蛋白4/人单级纺锤体1信号通路介导miR-21促进心肌纤维化的作用。方法 体外培养心肌成纤维细胞(CFs)并随机分为对照组、TGF-β1组、NC-miR-21组和TGF-β1+si-miR-21组;采用Western Bolt检测MAP4、p-MAP4、Mps1、p-Mps1、Ⅲ型胶原(Col Ⅲ)、Ⅰ型胶原(Col Ⅰ)、α平滑肌肌动蛋白(α-SMA);以试剂盒检测心肌细胞超氧化物歧化酶(SOD)和丙二醛(MDA)含量;酶联免疫吸附法(ELISA)检测各组细胞离心后的血清肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)及转化生长因子-β1(TGF-β1)水平。结果 TGF-β1组MDA、未折叠蛋白MAP4、p-MAP4、Mps1、p-Mps1、TNF-α、IL-6、TGF-β1、Col Ⅲ、ColⅠ及α-SMA胶原蛋白水平高于对照组,SOD水平低于对照组(P<0.05);TGF-β1+si-miR-21组MDA、未折叠蛋白MAP4、p-MAP4、Mps1、p-Mps1、TNF-α、IL-6、TGF-β1、Col Ⅲ、ColⅠ及α-SMA胶原蛋白水平低于NC-miR-21组,SOD水平高于NC-miR-21组(P<0.05)。结论 低表达miR-21可抑制心肌成纤维细胞Col Ⅲ、Col Ⅰ、α-SMA、TGF-β1的生物合成,其机制可能与Mps1/MAP4信号通路的激活调控有关。
Abstract:
Objective To investigate the role of microtubule-associated protein 4/human single spindle 1 signaling pathway in miR-21 promoting myocardial fibrosis.Methods Cardiac fibroblasts (CFs) were cultured in vitro and randomly divided into control group, TGF-β1 group, NC-miR-21 group and TGF-β1+si-miR-21 group. Western Bolt was used to detect MAP4, p-MAP4, Mps1, p-Mps1, collagen type Ⅲ (Col Ⅲ), collagen type Ⅰ (Col Ⅰ), α-smooth muscle actin (α-SMA); the contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in cardiomyocytes were detected by kit. The levels of serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and transforming growth factor-β1 (TGF-β1) were detected by enzyme-linked immunosorbent assay (ELISA).Results The levels of MDA, unfolded protein MAP4, p-MAP4, Mps1, p-Mps1, TNF-α, IL-6, TGF-β1, Col Ⅲ, Col Ⅰ and α-SMA collagen in TGF-β1 group were higher than those in control group, and the level of SOD was lower than that in control group (P<0.05). The levels of MDA, unfolded protein MAP4, p-MAP4, Mps1, p-Mps1, TNF-α, IL-6, TGF-β1, Col Ⅲ, Col Ⅰ and α-SMA collagen in TGF-β1+si-miR-21 group were lower than those in NC-miR-21 group, and the level of SOD was higher than that in NC-miR-21 group (P<0.05).Conclusion Down-regulation miR-21 can inhibit the biosynthesis of Col Ⅲ, Col Ⅰ, α-SMA and TGF-β1 in myocardial fibroblasts, and the mechanism may be related to the activation and regulation of Mps1/MAP4 signaling pathway.

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更新日期/Last Update: 1900-01-01