[1]李艳娜,王静一,陈琦蕴,等.B族链球菌基因cpsG影响人类宫颈癌细胞蛋白组学分析[J].医学信息,2023,36(14):8-15.[doi:10.3969/j.issn.1006-1959.2023.14.002]
　LI Yan-na,WANG Jing-yi,CHEN Qi-yun,et al.Proteomic Analysis of Group B Streptococcus Gene cpsG Affecting Human Cervical Cancer Cells[J].Journal of Medical Information,2023,36(14):8-15.[doi:10.3969/j.issn.1006-1959.2023.14.002]
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B族链球菌基因cpsG影响人类宫颈癌细胞蛋白组学分析()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年14期
页码:
8-15
栏目:
生物信息学
出版日期:
2023-07-15

文章信息/Info

Title:
Proteomic Analysis of Group B Streptococcus Gene cpsG Affecting Human Cervical Cancer Cells
文章编号:
1006-1959（2023）14-0008-08
作者:
李艳娜; 王静一; 陈琦蕴; 等
（同济大学医学院，上海 200092）
Author(s):
LI Yan-na; WANG Jing-yi; CHEN Qi-yun; et al.
(School of Medicine,Tongji University,Shanghai 200092,China)
关键词:
B族链球菌相关基因; cpsG; 人类宫颈癌细胞; 蛋白质组学; 质谱分析
Keywords:
Group B Streptococcus genes; cpsG; Human cervical cancer cells; Proteomics; Mass spectrometry analysis
分类号:
R34
DOI:
10.3969/j.issn.1006-1959.2023.14.002
文献标志码:
A
摘要:
目的 探讨B族链球菌基因cpsG影响人类宫颈癌细胞蛋白组学。方法 通过人工合成的方法将cpsG的读码框序列（ORF）融合HA标签连接到质粒pLVX-mCMV-ZsGreen的限制性内切酶EcoRI和BamHI之间构建稳定表达cpsG人类宫颈癌Hela细胞系。提取rLV组和rLV-cpsG组的总蛋白质，并通过12%SDS-PAGE电泳分析后，进行非标记（Label-free）蛋白质酶解肽段质谱分析。结果 经蛋白质酶解肽段质谱分析显示，rLV组鉴定出3552个蛋白，rLV-cpsG组鉴定出3702个蛋白；与rLV组相比，rLV-cpsG组中上调的蛋白为229个，下调的蛋白为225个。GO分析结果显示，细胞组分主要包括膜完整性、细胞核、核糖体；分子功能主要包括蛋白结合、ATP结合、GTP结合、DNA结合、RNA结合；生物过程主要包括翻译、代谢、蛋白磷酸化、跨膜。KEGG通路富集分析显示，Cellular Processes通路主要包括细胞生长与凋亡（179个）、细胞活力（72个）、真核细胞群（140个）、转运和代谢（270个）；Environmental Information Processing通路有膜转运（11个）、信号转导（324个）；Genetic Information Processing通路有复制与修复（51个）、转录（133个）、翻译（328个）；Human Diseases通路有癌症（258个）；Metabolism通路有氨基酸代谢（110个）、糖代谢（144个）；Organismal Systems通路包括内分泌系统（174个）。蛋白质-蛋白质相互作用结果显示，下调的蛋白互作网络主要包括prot1（MGST1）∶prot2（GCLC）、prot1（MGST1）∶prot2（MYH10）、prot1（MGST1）∶prot2（UGDH）、prot1（MGST1）∶prot2（GCLM）、prot1（PKP2）∶prot2（CDH2）、prot1（PKP2）∶prot2（A8K2T7）；上调的蛋白互作网络主要包括prot1（SLC7A2）∶prot2（TXN）、prot1（SLC7A2）∶prot2（KYNU）、prot1（SLC7A2）∶prot2（MCAT）、prot1（SLC7A2）∶prot2（JUNB）、prot1SLC7A2）∶prot2（BOLA2）、prot1（SLC7A2）∶prot2（FN1）。结论 B族链球菌基因cpsG影响了人类宫颈癌细胞的蛋白组学，该研究为明确cpsG在人类宫颈癌细胞中发生作用机制及其临床应用提供了基础资料。
Abstract:
Objective To investigate the effect of group B streptococcus gene cpsG on the proteomics of human cervical cancer cells.Methods A stable CPSG-expressing human cervical cancer Hela cell line was constructed by linking the cpsG Read frame sequence (ORF) fusion HA tag to the restriction enzymes EcoRI and BamHI of plasmid pLVX-mCMV-ZsGreen. The total proteins of rLV group and RLV-CPSG group were extracted and analyzed by 12% SDS-PAGE electrophoresis, then Label-free protein enzymolytic peptide mass spectrometry was performed.Results According to mass spectrometry, 3552 proteins were identified in rLV group and 3702 proteins were identified in rLV-cpsG group. Compared with the rLV group, 229 proteins were up-regulated and 225 proteins were down-regulated in the rLV-cpsG group. The results of GO analysis showed that the cell components mainly included membrane integrity, nucleus and ribosome; the molecular functions mainly include protein binding, ATP binding, GTP binding, DNA binding and RNA binding; biological processes mainly include translation, metabolism, protein phosphorylation, and transmembrane. Enrichment analysis of KEGG pathways showed that Cellular Processes mainly included cell growth and apoptosis (179), cell viability (72), eukaryotic cell population (140), and transport and metabolism (270); Environmental Information Processing pathways included membrane transport (11) and signal transduction (324); Genetic Information Processing pathways were replication and repair (51), transcription (133) and translation (328). There were cancers in the Human Diseases pathway (258); The Metabolism pathways include amino acid metabolism (110) and glucose metabolism (144). Organismal Systems channels include the endocrine system (174). The protein-protein interaction results showed that the down-regulated protein interaction networks mainly included prot1(MGST1)∶prot2(GCLC)、prot1(MGST1)∶prot2(MYH10)、prot1(MGST1)∶prot2(UGDH)、prot1(MGST1)∶prot2(GCLM)、prot1(PKP2)∶prot2(CDH2)、prot1(PKP2)∶prot2(A8K2T7); the up-regulated protein interaction networks mainly included prot1(SLC7A2)∶prot2(TXN)、prot1(SLC7A2)∶prot2(KYNU)、prot1(SLC7A2)∶prot2(MCAT)、prot1(SLC7A2)∶prot2(JUNB)、prot1SLC7A2)∶prot2(BOLA2)、prot1(SLC7A2)∶prot2(FN1).Conclusion Group B streptococcus gene cpsG affects the proteomics of human cervical cancer cells. This study provides basic data for clarifying the role of cpsG in human cervical cancer cells and its clinical application.

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更新日期/Last Update: 1900-01-01