[1]李得意,张路瑶,许 杨,等.基于生物信息学方法探究SERINC2基因在宫颈癌中的表达及其作用机制[J].医学信息,2023,36(14):1-7.[doi:10.3969/j.issn.1006-1959.2023.14.001]
　LI De-yi,ZHANG Lu-yao,XU Yang,et al.The Expression and Mechanism of SERINC2 Gene in Cervical Cancer Based on Bioinformatics Methods[J].Journal of Medical Information,2023,36(14):1-7.[doi:10.3969/j.issn.1006-1959.2023.14.001]
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基于生物信息学方法探究SERINC2基因在宫颈癌中的表达及其作用机制()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年14期
页码:
1-7
栏目:
生物信息学
出版日期:
2023-07-15

文章信息/Info

Title:
The Expression and Mechanism of SERINC2 Gene in Cervical Cancer Based on Bioinformatics Methods
文章编号:
1006-1959（2023）14-0001-07
作者:
李得意; 张路瑶; 许 杨; 等
（大理大学基础医学院，云南 大理 671000）
Author(s):
LI De-yi; ZHANG Lu-yao; XU Yang; et al.
(Basic Medical College of Dali University,Dali 671000,Yunnan,China)
关键词:
宫颈癌; SERINC2; 甲基化; 糖代谢; 生物信息学分析
Keywords:
Cervical cancer; SERINC2; Methylation; Glucose metabolism; Bioinformatics analysis
分类号:
R737.33
DOI:
10.3969/j.issn.1006-1959.2023.14.001
文献标志码:
A
摘要:
目的 通过生物信息学方法分析SERINC2在宫颈癌中的表达及其功能特性。方法 利用基因表达谱数据动态分析（GEPIA）探究SERINC2基因在宫颈癌及癌旁组织的表达，评价其与宫颈癌患者的生存预后关系；并在人蛋白质数据库（HPA）中获取SERINC2蛋白在宫颈癌和癌旁组织的表达情况；基于Ualcan数据库分析SERINC2甲基化水平与宫颈癌临床病理特征之间的相关性；利用Linked Omics数据库探索SERINC2的共表达基因，并进一步分析SERINC2基因在宫颈癌发展过程中可能参与的信号调控通路。结果 宫颈癌组织中SERINC2基因表达高于癌旁组织（P＜0.05），且与患者不良预后相关。宫颈癌组织中的SERINC2甲基化水平低于癌旁组织（P＜0.05）。共表达结果分析显示，GALE、GPRC5A、TINAGL1、C9orf167和CAP1等基因与SERINC2表达呈正相关，SCML2、KDM2B、GLS2、ARHGAP19和SCAL等基因与其表达呈负相关（FDR＜0.05）；SERINC2基因参与了半乳糖代谢、氨基糖和核苷酸糖代谢、聚糖的合成和降解等一系列与糖代谢相关的过程（P＜0.05，FDR＜0.25）。结论 SERINC2基因在宫颈癌中高表达，与宫颈癌的发生发展及不良预后有关，SERINC2基因可能成为宫颈癌预后评估和治疗的靶点。
Abstract:
Objective To analyze the expression and functional characteristics of SERINC2 in cervical cancer by bioinformatics.Methods The expression of SERINC2 gene in cervical cancer and adjacent tissues was explored by Gene Expression Profiling Interactive Analysis (GEPIA), and its relationship with the survival and prognosis of patients with cervical cancer was evaluated. The expression of SERINC2 protein in cervical cancer and adjacent tissues was obtained in the Human Protein Atlas (HPA). The correlation between SERINC2 methylation level and clinicopathological features of cervical cancer was analyzed based on Ualcan database. Using the Linked Omics database to explore the co-expressed genes of SERINC2, and further analyze the signal regulatory pathways that SERINC2 gene may participate in in the development of cervical cancer.Results The expression of SERINC2 gene in cervical cancer tissues was higher than that in adjacent tissues (P<0.05), and it was related to the poor prognosis of patients. The methylation level of SERINC2 in cervical cancer tissues was lower than that in adjacent tissues (P<0.05). The results of co-expression analysis showed that GALE, GPRC5 A, TINAGL1, C9orf167 and CAP1 were positively correlated with SERINC2 expression, while SCML2, KDM2 B, GLS2, ARHGAP19 and SCAL were negatively correlated with SERINC2 expression (FDR<0.05). SERINC2 gene was involved in a series of processes related to glucose metabolism, such as galactose metabolism, amino sugar and nucleotide sugar metabolism, glycan synthesis and degradation (P<0.05, FDR<0.25).Conclusion SERINC2 gene is highly expressed in cervical cancer, which is associated with the development and poor prognosis of cervical cancer. SERINC2 gene may be a target for prognosis evaluation and treatment of cervical cancer.

参考文献/References:

[1]Merera D,Jima GH.Precancerous Cervical Lesions and Associated Factors Among Women Attending Cervical Screening at Adama Hospital Medical College,Central Ethiopia[J].Cancer Manag Res,2021,13:2181-2189.[2]刘佳琪,李朋飞,纪妹,等.基于中国子宫颈癌临床诊疗大数据的子宫颈癌诊疗规范化调查分析[J].中国实用妇科与产科杂志,2021,37(1):82-86.[3]da Costa SCS,Bonadio RC,Gabrielli FCG,et al.Neoadjuvant Chemotherapy With Cisplatin and Gemcitabine Followed by Chemoradiation Versus Chemoradiation for Locally Advanced Cervical Cancer: A Randomized Phase II Trial[J].J Clin Oncol,2019,37(33):3124-3131.[4]Inuzuka M,Hayakawa M,Ingi T.Serinc,an activity-regulated protein family,incorporates serine into membrane lipid synthesis[J].J Biol Chem,2005,280(42):35776-35783.[5]Ren WH,Yang CY,Yang XM,et al.siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21[J].Oncol Rep,2014,31(3):1314-1322.[6]Chang GH,Barbaro NM,Pieper RO.Phosphatidylserine-dependent phagocytosis of apoptotic glioma cells by normal human microglia,astrocytes,and glioma cells[J].Neuro Oncol,2000,2(3):174-183.[7]Liang Z,Riaz A,Chachar S,et al.Epigenetic Modifications of mRNA and DNA in Plants[J].Mol Plant,2020,13(1):14-30.[8]Carroll DJ.Integrating experience with databases,bioinformatics,and wet lab exercises for students in an introductory genetics course[J].Biochem Mol Biol Educ,2022,50(5):457-459.[9]Orton RJ,Gu Q,Hughes J,et al.Bioinformatics tools for analysing viral genomic data[J].Rev Sci Tech,2016,35(1):271-285.[10]Zhao XT,Zhu Y,Zhou JF,et al.Development of a novel 7 immune-related genes prognostic model for oral cancer: A study based on TCGA database[J].Oral Oncol,2021,112:105088.[11]Cao W,Chen HD,Yu YW,et al.Changing profiles of cancer burden worldwide and in China: a secondary analysis of the global cancer statistics 2020[J].Chin Med J (Engl),2021,134(7):783-791.[12]de Sanjosé S,Serrano B,Castellsagué X,et al.Human papillomavirus (HPV) and related cancers in the Global Alliance for Vaccines and Immunization (GAVI) countries.A WHO/ICO HPV Information Centre Report[J].Vaccine,2012,30 Suppl 4:D1-83,vi.[13]Xu S,Zheng Z,Pathak JL,et al.The Emerging Role of the Serine Incorporator Protein Family in Regulating Viral Infection[J].Front Cell Dev Biol,2022,10:856468.[14]Digumarti R,Bapsy PP,Suresh AV,et al.Bavituximab plus paclitaxel and carboplatin for the treatment of advanced non-small-cell lung cancer[J].Lung Cancer,2014,86(2):231-236.[15]Yin Y,Huang X,Lynn KD,et al.Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation[J].Cancer Immunol Res,2013,1(4):256-268.[16]Bondanza A,Zimmermann VS,Rovere-Querini P,et al.Inhibition of phosphatidylserine recognition heightens the immunogenicity of irradiated lymphoma cells in vivo[J].J Exp Med,2004,200(9):1157-1165.[17]Kok JW,Sietsma H.Sphingolipid metabolism enzymes as targets for anticancer therapy[J].Curr Drug Targets,2004,5(4):375-382.[18]Proia RL,Hla T.Emerging biology of sphingosine-1-phosphate: its role in pathogenesis and therapy[J].J Clin Invest,2015,125(4):1379-1387.[19]Ogretmen B,Hannun YA.Biologically active sphingolipids in cancer pathogenesis and treatment[J].Nat Rev Cancer,2004,4(8):604-616.[20]Zeng Y,Xiao D,He H,et al.SERINC2-knockdown inhibits proliferation,migration and invasion in lung adenocarcinoma[J].Oncol Lett,2018,16(5):5916-5922.[21]Pan Y,Liu G,Zhou F,et al.DNA methylation profiles in cancer diagnosis and therapeutics[J].Clin Exp Med,2018,18(1):1-14.[22]傅柳陶,王青元,卫兵,等.PGAM1通过激活Warburg效应调控宫颈癌细胞恶性生物学行为的机制研究[J].安徽医科大学报,2018,53(9):1398-1402.[23]Broussard A,Florwick A,Desbiens C,et al.Human UDP-galactose 4’-epimerase (GALE) is required for cell-surface glycome structure and function[J].J Biol Chem,2020,295(5):1225-1239.[24]da Silveira Mitteldorf CA,de Sousa-Canavez JM,Leite KR,et al.FN1,GALE,MET,and QPCT overexpression in papillary thyroid carcinoma:molecular analysis using frozen tissue and routine fine-needle aspiration biopsy samples[J].Diagn Cytopathol,2011,39(8):556-561.[25]孙晓朋.miR-let-7i-5p靶向GALE抑制人胶质母细胞瘤增殖和侵袭迁移特性的机制研究[D].济南:山东大学,2021.

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更新日期/Last Update: 1900-01-01