[1]杜雅静,王宇意,章丹婷,等.研磨珠均质仪提取增生性瘢痕组织蛋白质的方法[J].医学信息,2018,31(15):51-54.[doi:10.3969/j.issn.1006-1959.2018.15.017]
　DU Ya-jing,WANG Yu-yi,ZHANG Dan-ting,et al.Method for Extracting Hyperplastic Scar Tissue Protein by Grinding Bead Homogenizer[J].Journal of Medical Information,2018,31(15):51-54.[doi:10.3969/j.issn.1006-1959.2018.15.017]
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研磨珠均质仪提取增生性瘢痕组织蛋白质的方法()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
31卷
期数:
2018年15期
页码:
51-54
栏目:
论著
出版日期:
2018-08-01

文章信息/Info

Title:
Method for Extracting Hyperplastic Scar Tissue Protein by Grinding Bead Homogenizer
文章编号:
1006-1959（2018）15-0051-04
作者:
杜雅静1; 王宇意1; 章丹婷1; 郭丽微1; 方 杰1; 高梦炜1; 何文涓2; 钟晓春1
1.杭州师范大学医学院，浙江 杭州 310036； 2.无锡卫生高等职业技术学校，江苏 无锡 214028
Author(s):
DU Ya-jing1; WANG Yu-yi1; ZHANG Dan-ting1; GUO Li-wei1; FANG Jie1; GAO Meng-wei1; HE Wen-juan2;  ZHONG Xiao-chun1
1.Hangzhou Normal University Medical College,Hangzhou 310036,Zhejiang,China; 2.Wuxi Health Vocational and Technical School,Wuxi 214028,Jiangsu,China
关键词:
增生性瘢痕; 前列腺素; 蛋白提取; 研磨珠均质仪
Keywords:
Key words：Hyperplastic scar; Prostaglandin; Protein extraction; Grinding bead homogenizer
分类号:
R622+.1
DOI:
10.3969/j.issn.1006-1959.2018.15.017
文献标志码:
A
摘要:
目的 利用研磨珠均质仪，从临床增生性瘢痕组织样本中充分提取蛋白质。方法 临床收集烧伤后增生性瘢痕组织样本，通过玻璃匀浆器、研磨珠均质仪等方法，提取样本总蛋白，比较提取后残渣，BCA测定总蛋白浓度，蛋白凝胶电泳考马斯亮蓝染色观察蛋白质条带完整性，和western blot比较前列腺素D合成酶的含量（PTGDS）。结果 玻璃匀浆器与研磨珠均质仪联合方法残渣呈絮状均匀，获得蛋白质量最大，差异具有统计学意义（P＜0.05），蛋白凝胶电泳考马斯亮蓝染色样本均未降解，Western blot测得联合方法提取样本中前列腺素D合成酶含量更高。结论 玻璃匀浆器与研磨珠均质仪的联合提取临床增生性瘢痕组织样本中获得蛋白质较为高效，是一种临床瘢痕组织蛋白样本提取方法。
Abstract:
Abstract：Objective To fully extract proteins from clinical hyperplastic scar tissue samples using a bead homogenizer.Methods The samples of hyperplastic scar tissue after burn were collected clinically,and the total protein of the sample was extracted by means of glass homogenizer and grinding bead homogenizer.The residue after extraction was compared.The total protein concentration was determined by BCA.Protein gel electrophoresis was performed by Coomassie blue staining.Band integrity,and prostaglandin D synthetase content(PTGDS)compared to western blot.Results The residue of the glass homogenizer and the grinding bead homogenizer was flocculated uniformly,and the protein content was the largest,which was statistically significant(P<0.05).The protein gel electrophoresis Coomassie blue stained samples were not degraded,and Western blot was used.The combined method of extracting samples has higher levels of prostaglandin D synthetase.Conclusion The combination of glass homogenizer and grinding bead homogenizer to obtain protein in clinical hyperplastic scar tissue samples is more efficient,and it is a method for extracting clinical scar tissue protein samples.

参考文献/References:

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更新日期/Last Update: 2018-08-01