[1]郑 梅,梁广忠,赵晓萌,等.倒置显微镜观察MDCK细胞分离流感病毒 过程中的状态变化[J].医学信息,2019,32(16):163-164.[doi:10.3969/j.issn.1006-1959.2019.16.055]
　ZHENG Mei,LIANG Guang-zhong,ZHAO Xiao-meng,et al.Inverted Microscope to Observe the State Changes in the Process of Isolation of Influenza Virus by MDCK Cells[J].Journal of Medical Information,2019,32(16):163-164.[doi:10.3969/j.issn.1006-1959.2019.16.055]
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倒置显微镜观察MDCK细胞分离流感病毒 过程中的状态变化()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年16期
页码:
163-164
栏目:
诊疗技术
出版日期:
2019-08-15

文章信息/Info

Title:
Inverted Microscope to Observe the State Changes in the Process of Isolation of Influenza Virus by MDCK Cells
文章编号:
1006-1959（2019）16-0163-02
作者:
郑 梅; 梁广忠; 赵晓萌; 孙雅娜; 赵 颖; 杨振河
天津市津南区疾病预防控制中心，天津 300000
Author(s):
ZHENG Mei; LIANG Guang-zhong; ZHAO Xiao-meng; SUN Ya-na; ZHAO Ying; YANG Zhen-he
Tianjin Jinnan District Center for Disease Control and Prevention,Tianjin 300000,China
关键词:
倒置显微镜; 流感病毒; MDCK细胞
Keywords:
Key words：Inverted microscope; Influenza virus; MDCK cells
分类号:
R373.1
DOI:
10.3969/j.issn.1006-1959.2019.16.055
文献标志码:
A
摘要:
目的 探讨倒置显微镜下观察狗肾上皮细胞（MDCK）分离流感病毒状态变化，为科学防控流感提供技术支持。方法 选取天津市津南区疾病预防控制中心流感网络实验室于2018年10月11日~20日流感病毒检测阳性的咽拭子样本20例，接种到MDCK传代细胞中，于接种后使用倒置显微镜观察细胞形态变化，包括细胞肿胀、间隙增大、核固缩或破裂、细胞部分或全部脱落，用血凝实验方法测定MDCK细胞不同分离状态下的流感病毒滴度状况。结果 倒置显微镜可以观察到MDCK细胞分离流感病毒状态的变化，阴性对照与未出现倒置显微镜镜下病变的MDCK细胞不能使人“O”型红细胞凝集；出现病变的MDCK细胞可以使人“O”型红细胞凝集，产生HA滴度。结论 倒置显微镜在观察MDCK细胞分离流感病毒过程中起到重要作用，可提前判断病毒的滴度与活力，为科学防控流感提供技术支持。
Abstract:
Abstract：Objective To investigate the changes of influenza virus status in dog kidney epithelial cells (MDCK) under inverted microscope and provide technical support for scientific prevention and control of influenza. Methods 20 cases of swallow swab samples with positive influenza virus detection were selected from the influenza network laboratory of Tianjin Jinnan District Center for Disease Control and Prevention from October 11 to 20, 2018. They were inoculated into MDCK passage cells and observed under inverted microscope after inoculation. Cell morphology changes, including cell swelling, gap enlargement, nuclear pyknosis or rupture, partial or total detachment of cells, blood clotting experiments were used to determine the influenza virus titer status of MDCK cells under different separation conditions. Results Inverted microscope can observe the changes of MDCK cells in the state of influenza virus isolation. Negative control and MDCK cells without inverted microscopic lesions cannot agglutinate human "O" type red blood cells; MDCK cells with lesions can make human "O" type Red blood cells aggregate to produce HA titers. Conclusion Inverted microscope plays an important role in the observation of MDCK cells in the isolation of influenza virus. It can judge the titer and vitality of virus in advance and provide technical support for scientific prevention and control of influenza.

参考文献/References:

[1]郭丽茹,孔梅,李晓燕,等.2010年-2013年天津市流感病原学监测分析[J].中国卫生检验杂志,2013,23(13):2795-2797. [2]董晓春,李琳,徐文体,等.流感病毒MDCK细胞分离阳性率的影响因素分析[J].中国卫生检验杂志,2014,24(11):1582-1583,1587. [3]马萍,李欣玥.流感病毒在狗肾上皮细胞上的最优培养条件[J].现代预防医学,2017,44(23):4326-4331. [4]崔志英.倒置显微镜技术发展趋势[J].科技传播,2016,8(13):148-149. [5]许沙沙,田鹏,曹开源.甲型流感病毒的研究进展[J].热带医学杂志,2015,15(10):1450-1452,1455.

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更新日期/Last Update: 2019-08-15