[1]刘一岚,帅燕蓉,邓 锐,等.碱性成纤维细胞生长因子调节小鼠慢性移植物抗宿主病的作用及其机制[J].医学信息,2021,34(06):78-81.[doi:10.3969/j.issn.1006-1959.2021.06.020]
　LIU Yi-lan,SHUAI Yan-rong,DENG Rui,et al.Effect Basic Fibroblast Growth Factor Regulates Chronic Graft Versus Host Disease in Mice and Its Mechanism[J].Medical Information,2021,34(06):78-81.[doi:10.3969/j.issn.1006-1959.2021.06.020]
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碱性成纤维细胞生长因子调节小鼠慢性移植物抗宿主病的作用及其机制()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年06期
页码:
78-81
栏目:
论著
出版日期:
2021-03-15

文章信息/Info

Title:
Effect Basic Fibroblast Growth Factor Regulates Chronic Graft Versus Host Disease in Mice and Its Mechanism
文章编号:
1006-1959（2021）06-0078-04
作者:
刘一岚; 帅燕蓉; 邓 锐; 等
（解放军西部战区总医院血液科，四川 成都 610038）
Author(s):
LIU Yi-lan; SHUAI Yan-rong; DENG Rui; et al.
(Department of Hematology,General Hospital of the Western Theater Command of the PLA, Chengdu 610038,Sichuan,China)
关键词:
碱性成纤维细胞生长因子; 慢性移植物抗宿主病; 间充质干细胞
Keywords:
Basic fibroblast growth factor; Chronic graft versus host disease; Mesenchymal stem cells
分类号:
R552
DOI:
10.3969/j.issn.1006-1959.2021.06.020
文献标志码:
A
摘要:
目的 分析碱性成纤维细胞生长因子（bFGF）调节小鼠慢性移植物抗宿主病（cGVHD）的作用及其机制。方法 通过外源性MSC培养传代和分离小鼠脾脏淋巴细胞建立单倍体HSCT后cGVHD的小鼠模型，将实验设立空白对照组（n=12）、对照组（n=12）和实验组（n=12）。空白对照组移植后不采取任何预防cGVHD措施，对照组移植后每只小鼠每天输注20 μg/kg外源性MSC，实验组移植后每只小鼠每天注射100 μg/kg bFGF，比较三组小鼠生存情况和cGVHD临床表现，采用流式细胞术cGVHD相关免疫细胞检测CD4+和CD25+细胞水平，采用ELISA酶联免疫细胞因子检测IL-10、IL-12、TGF-β和TNF-α水平，观察间充质干细胞培养结果和CD44、CD31表达结果。结果 实验组小鼠生存时间多于空白对照组和对照组，cGVHD临床症状出现时间晚于对照组和空白对照组，且移植后3 d白细胞计数比较，差异无统计学意义（P＞0.05）；三组移植后6、9、12、14、15 d白细胞计数，移植后7、14 d cGVHD体重减轻情况，IL-10、IL-12、TNF-β和TNF-α水平，CD4+和CD25+细胞比较，差异有统计学意义（P＜0.05）。间充质干细胞培养显示，移植后14 d，对照组和空白对照组细胞无法集落和继续生长，而实验组可见大量细胞集落且良好生长，光镜下见细胞呈长梭状且贴壁，细胞核紧密团结。结论 bFGF对cGVHD具有调节作用，其通过促进单倍体HSCT供者来源的间充质干细胞体内增殖，使调节性T细胞、调节性B细胞和骨髓来源的抑制细胞增殖，从而调节cGVHD，延缓其发生。
Abstract:
Objective To analyze the effect and mechanism of basic fibroblast growth factor (bFGF) in regulating chronic graft-versus-host disease (cGVHD) in mice.Methods A mouse model of cGVHD after haploid HSCT was established by culture and passage of exogenous MSC and isolation of mouse spleen lymphocytes. The experiment was set up as a blank control group (n=12), a control group (n=12) and an experimental group (n=12).After transplantation, the blank control group did not take any measures to prevent cGVHD. After transplantation, each mouse in the control group was infused with 20 μg/kg exogenous MSC per day, and each mouse in the experimental group was injected with 100 μg/kg bFGF per day after transplantation.The survival conditions and clinical manifestations of cGVHD in the three groups of mice were compared, and the levels of CD4+ and CD25+ cells were detected by flow cytometry cGVHD-related immune cells.ELISA was used to detect the levels of IL-10, IL-12, TGF-β and TNF-α, and observe the results of mesenchymal stem cell culture and the expression of CD44 and CD31.Results The survival time of mice in the experimental group was longer than that of the blank control group and the control group, and the clinical symptoms of cGVHD appeared later than the control group and the blank control group. There was no significant difference in white blood cell counts 3 d after transplantation (P>0.05);The white blood cell counts of the three groups at 6, 9, 12, 14, 15 d after transplantation, the weight loss of cGVHD at 7, 14 d after transplantation, the levels of IL-10, IL-12, TNF-β and TNF-α, and the comparison of CD4+ and CD25+ cells,the difference was statistically significant (P<0.05).The mesenchymal stem cell culture showed that 14 d after transplantation, the control group and blank control group could not colonize and continue to grow, while the experimental group saw a large number of cell colonies and grew well.Under the light microscope, the cells were long spindle-shaped and adherent, and the nuclei were tightly united.Conclusion bFGF has a regulatory effect on cGVHD. It promotes the proliferation of mesenchymal stem cells derived from haploid HSCT donors, and makes regulatory T cells, regulatory B cells and bone marrow-derived suppressor cells proliferate, thereby regulating cGVHD and delaying it occur.

参考文献/References:

[1]Nunes NS,Kanakry CG.Mechanisms of Graft-versus-Host Disease Prevention by Post-transplantation Cyclophosphamide:An Evolving Understanding[J].Front Immunol,2019(10):2668. [2]许志锋,李敬来,韩振,等.碱性成纤维细胞生长因子基因修饰骨骼肌卫星细胞自体移植急性心肌梗死组织的血管新生[J].中国组织工程研究,2017,21(9):1397-1401. [3]蒋依琳,高洪瑞.个体化综合治疗慢性移植物抗宿主病相关干眼的临床研究[J].眼科新进展,2017,37(10):958-961. [4]于宁,王彦生,齐常萍.碱性成纤维细胞生长因子基因转染骨髓间充质干细胞在失神经肌肉萎缩中的应用[J].中国组织工程研究,2016,20(1):89-94. [5]Khoder A,Sarvaria A,Alsuliman A.Regulatory B cells are enriched within the IgM memory and transitional subsets in healthy donors but are deficient in chronic GVHD[J].Blood,2014,124(13):2034-2045. [6]仇利红,韩悦,郑惠,等.碱性成纤维细胞生长因子联合血管内皮细胞生长因子促进自体脂肪移植成活的动物实验研究[J].中国美容整形外科杂志,2016,27(12):759-762. [7]崔利德,李铁民.碱性成纤维细胞生长因子基因修饰骨髓间充质干细胞移植修复急性肾损伤[J].中国组织工程研究,2016,20(28):4169-4175. [8]黄江南,张文宇,焦杨.碱性成纤维细胞生长因子21与心血管疾病关系的研究进展[J].广西医科大学学报,2018,35(7):1022-1025. [9]孙艳,蔡雁.碱性成纤维细胞生长因子在产科疾病中的研究进展[J].医学综述,2016,22(15):2946-2949. [10]孔庆玲,陈伟,苏贵珍,等.Caspase1抑制剂Ac-YVAD-CMK在防治小鼠急性移植物抗宿主病中的作用[J].中国实验血液学杂志,2017,25(1):203-208. [11]吕长遥,伍静,龚翰林.六合丹对家兔皮肤感染创面愈合过程中SP MMP-1及bFGF表达的影响[J].西部医学,2017,29(11):1492-1497. [12]李潇,王昀,陈韬亮,等.靶向成纤维细胞生长因子受体抑制胶质瘤中血管生成拟态的生成[J].实用医学杂志,2017,33(11):1735-1738. [13]高茜,刘丹,彭雷,等.野黄芩苷对苯并芘致人胚肺成纤维细胞DNA损伤的保护作用[J]. 医学分子生物学杂志,2018(1):30-34. [14]王雪梦,吴雷,顾绍庆.人巨细胞病毒感染对人胚肺成纤维细胞自噬的影响[J].江苏大学学报(医学版),2017,27(5):416-419. [15]邹力勇,胥光热,刘丽.骨化三醇可抑制大鼠心肌间质成纤维细胞增殖[J].西部医学,2017,29(9):1208-1210. [16]邓陶然,肖毅.慢性移植物抗宿主病的治疗进展[J].器官移植,2016,7(1):67-71. [17]王飞雁,高春记.慢性移植物抗宿主病的治疗进展[J].中国实验血液学杂志,2017,25(1):286-290. [18]黄河,周道斌,于力,等.原始骨髓间充质干细胞治疗慢性移植物抗宿主病临床研究方案[J].基础医学与临床,2017,37(3):296-299. [19]聂倩倩,朱紫菱,姜支农,等.原代癌相关成纤维细胞和正常成纤维细胞特征比较及对结直肠癌细胞EMT影响[J].医学分子生物学杂志,2016,13(3):136-142. [20]徐文倩,黄岩彬,沙苗,等.阳离子化金针菇多糖的制备及其在大鼠胚胎成纤维细胞中的基因转染效率评价[J].江苏大学学报(医学版),2017,27(4):344-349.

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更新日期/Last Update: 1900-01-01