[1]李继荣,王志坚,陶保菊.IL-17A基因-197A/G位点顺式调控机制及其对IL-17表达的影响[J].医学信息,2023,36(01):95-98.[doi:10.3969/j.issn.1006-1959.2023.01.018]
 LI Ji-rong,WANG Zhi-jian,TAO Bao-ju.The Cis-regulatory Mechanism of IL-17A Gene-197A/G Site and its Effect on IL-17 Expression[J].Journal of Medical Information,2023,36(01):95-98.[doi:10.3969/j.issn.1006-1959.2023.01.018]
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IL-17A基因-197A/G位点顺式调控机制及其对IL-17表达的影响()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
36卷
期数:
2023年01期
页码:
95-98
栏目:
论著
出版日期:
2023-01-01

文章信息/Info

Title:
The Cis-regulatory Mechanism of IL-17A Gene-197A/G Site and its Effect on IL-17 Expression
文章编号:
1006-1959(2023)01-0095-04
作者:
李继荣王志坚陶保菊
(南部战区海军第二医院感染科,海南 三亚 572000)
Author(s):
LI Ji-rongWANG Zhi-jianTAO Bao-ju
(Department of Infectious Diseases,the Second Naval Hospital,Southern Theater Command,Sanya 572000,Hainan,China)
关键词:
单核苷酸多态性基因调控顺式调控
Keywords:
Single nucleotide polymorphismGene regulationCis-regulation
分类号:
Q344+.14
DOI:
10.3969/j.issn.1006-1959.2023.01.018
文献标志码:
A
摘要:
目的 探讨IL-17A基因-197A/G位点顺式调控作用及其对IL-17表达的影响。方法 采用TaqMan系统对我院血液中心2021年2月1日-6月1日406名健康献血员全血样本进行IL-17A基因-197A/G位点基因分型,采用酶联免疫吸附试剂盒测定不同基因型个体血清IL-17水平,另通过电泳迁移率实验观察IL-17A基因-197A/G位点顺式调控作用。结果 不同性别基因型分布及等位频率比较,差异无统计学意义(P>0.05);不同基因型个体间IL-17水平比较,差异有统计学意义(P<0.05),其中IL-17水平随基因型由AA[(296.12±63.43)pg/ml]→AG[(262.43±56.14)pg/ml]→GG[(159.17±31.46)pg/ml]变化呈递减趋势;电泳迁移率实验显示,一未知核因子与IL-17A基因-197A/G位点(rs2275913)A等位及G等位DNA探针特异性结合,但亲和力差异显著(A等位>G等位)。结论 IL-17A基因-197A/G位点是一个非常关键的功能位点,通过特异性与一未知核转录因子结合而发挥对IL-17表达的顺式调控作用。
Abstract:
Objective To investigate the cis-regulation mechanism of IL-17A gene-197A/G site and its effect on IL-17 expression.Methods The TaqMan system was used to genotype the IL-17A gene-197A / G locus in the whole blood samples of 406 healthy blood donors in the blood center of our hospital from February 1 to June 1,2021. The serum IL-17 levels of individuals with different genotypes were determined by enzyme-linked immunosorbent assay kit. In addition, the cis-regulation of IL-17A gene-197A/G locus was observed by electrophoretic mobility assay.Results There was no significant difference in genotype distribution and allele frequency between different genders (P>0.05). There were significant differences in IL-17 levels among different genotypes (P<0.05), while the level of IL-17 decreased with the change of genotype from AA[(296.12±63.43) pg/ml]→AG[(262.43±56.14)pg/ml]→GG[(159.17±31.46)pg/ml]. Electrophoretic mobility shift assay showed that an unknown nuclear factor could specifically bind to A and G allelic DNA probes of IL-17A gene-197A/G site (rs2275913), but the affinity was significantly different (A allelic>G allelic).Conclusion The IL-17A gene-197A/G site is a very critical functional site, which plays a cis-regulatory role in IL-17 expression by specifically binding to an unknown nuclear transcription factor.

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更新日期/Last Update: 1900-01-01