[1]徐 光,晏三妹,徐 锐.细菌常量生化鉴定法联合荧光定量PCR检测对多种食源性致病菌的鉴别检测价值[J].医学信息,2026,39(10):135-139.[doi:10.3969/j.issn.1006-1959.2026.10.024]
　XU Guang,YAN Sanmei,XU Rui.Value of Routine Bacterial Biochemical Identification Method Combined with Fluorescent Quantitative PCR for the Identification and Detection of Multiple Foodborne Pathogens[J].Journal of Medical Information,2026,39(10):135-139.[doi:10.3969/j.issn.1006-1959.2026.10.024]
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细菌常量生化鉴定法联合荧光定量PCR检测对多种食源性致病菌的鉴别检测价值()

医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
39卷
期数:
2026年10期
页码:
135-139
栏目:
临床证据信息
出版日期:
2026-05-15

文章信息/Info

Title:
Value of Routine Bacterial Biochemical Identification Method Combined with Fluorescent Quantitative PCR for the Identification and Detection of Multiple Foodborne Pathogens
文章编号:
1006-1959（2026）10-0135-05
作者:
徐 光1; 晏三妹2; 徐 锐3
1.上高县疾病预防控制中心检验科，江西 上高 336400；2.上高县人民医院检验科，江西 上高 336400；3.辽宁中医药大学杏林学院中医系，辽宁 沈阳 110000
Author(s):
XU Guang1;  YAN Sanmei2;  XU Rui3
1.Department of Clinical Laboratory, Shanggao County Center for Disease Control and Prevention, Shanggao 336400, Jiangxi, China;2.Department of Clinical Laboratory, Shanggao County People′s Hospital, Shanggao 336400, Jiangxi, China;3.Department of Traditional Chinese Medicine, Xinglin College, Liaoning University of Traditional Chinese Medicine,Shenyang 110000, Liaoning, China
关键词:
细菌常量生化鉴定法; 荧光定量PCR检测; 食源性致病菌
Keywords:
Routine bacterial biochemical identification method;  Fluorescence quantitative PCR detection;  Foodborne pathogen
分类号:
R155.5
DOI:
10.3969/j.issn.1006-1959.2026.10.024
文献标志码:
A
摘要:
目的 探讨细菌常量生化鉴定法联合荧光定量普通聚合酶链式反应（PCR）检测对多种食源性致病菌的鉴别检测价值。方法 选取2022年1月-2024年12月于上高县疾病预防控制中心采集的100份食品样本，均进行细菌常量生化鉴定法与荧光定量PCR检测，以金标准（微生物培养鉴定法）结果为对照，比较两种方法对沙门氏菌、金黄色葡萄球菌、大肠杆菌、志贺氏菌、李斯特菌的检测准确率、灵敏度、特异度及检测时间。结果 细菌常量生化鉴定法联合荧光定量PCR检测对沙门氏菌、金黄色葡萄球菌、大肠杆菌、志贺氏菌、李斯特菌的检测准确率、灵敏度、特异度均高于单一检测方法，且检测时间短于细菌常量生化鉴定法，与金标准符合率也高于单一检测方法（P＜0.05）。结论 细菌常量生化鉴定法联合荧光定量PCR检测可显著提高多种食源性致病菌的检出率，缩短检测时间，在食源性致病菌检测中具有重要的应用价值。
Abstract:
Objective To investigate the value of routine bacterial biochemical identification method combined with fluorescent quantitative polymerase chain reaction (PCR) in the differential detection of multiple foodborne pathogens. Methods A total of 100 food samples collected at the Shanggao County Center for Disease Control and Prevention from January 2022 to December 2024 were selected. All samples were tested by routine bacterial biochemical identification method and fluorescent quantitative PCR. Using the results of the gold standard (microbial culture identification method) as the reference, the two methods were compared in terms of detection accuracy, sensitivity, specificity, and detection time for Salmonella, Staphylococcus aureus, Escherichia coli, Shigella, and Listeria. Results The detection accuracy, sensitivity, and specificity of routine bacterial biochemical identification method combined with fluorescent quantitative PCR for Salmonella, Staphylococcus aureus, Escherichia coli, Shigella, and Listeria were all higher than those of either single detection method. Moreover, the combined method had a shorter detection time than routine bacterial biochemical identification method alone, and its agreement rate with the gold standard was also higher than that of either single method alone (P<0.05). Conclusion Routine bacterial biochemical identification method combined with fluorescent quantitative PCR can significantly improve the detection rate of multiple foodborne pathogens and shorten the detection time, demonstrating important application value in the detection of foodborne pathogens.

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更新日期/Last Update: 1900-01-01