[1]李 江.细菌培养与PCR在细菌性痢疾检测中的应用效果比较[J].医学信息,2019,32(17):176-177.[doi:10.3969/j.issn.1006-1959.2019.17.060]
　LI Jiang.Comparison of Bacterial Culture and PCR in the Detection of Bacterial Dysentery[J].Journal of Medical Information,2019,32(17):176-177.[doi:10.3969/j.issn.1006-1959.2019.17.060]
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细菌培养与PCR在细菌性痢疾检测中的应用效果比较()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年17期
页码:
176-177
栏目:
诊疗技术
出版日期:
2019-09-01

文章信息/Info

Title:
Comparison of Bacterial Culture and PCR in the Detection of Bacterial Dysentery
文章编号:
1006-1959（2019）17-0176-02
作者:
李 江
湖北省荆州市第三人民医院检验科，湖北 荆州 434000
Author(s):
LI Jiang
Department of Clinical Laboratory,the Third People's Hospital of Jingzhou,Jingzhou 434000,Hubei,China
关键词:
细菌培养; 聚合酶链反应; 细菌性痢疾
Keywords:
Key words：Bacterial culture; Polymerase chain reaction; Bacterial dysentery
分类号:
R516.4
DOI:
10.3969/j.issn.1006-1959.2019.17.060
文献标志码:
A
摘要:
目的 对比细菌培养与聚合酶链反应（PCR）检测在细菌性痢疾检测中的应用效果。方法 选取2018年1月~2019年1月在我院诊治的220例腹泻患者作为研究对象，分别采用细菌培养与聚合酶链反应（PCR）对其粪便标本进行检测，对比两种方法检出率及患者基本情况对检出率的影响。结果 PCR检出率50.45%高于细菌培养4.54%，且不同年龄患者PCR的检出率均高于细菌培养法，差异有统计学意义（P＜0.05）；在PCR检测中，培养阴性、非脓血便、年龄≥5岁与培养阳性、脓血便、年龄＜5岁病例PCR平均循环数对比，差异有统计学意义（P＜0.05）。结论 在细菌性痢疾的检测中PCR检测与细菌培养对比具有更准确的评估作用，利于临床对细菌性痢疾人群的评估，具有临床应用的重要意义。同时不同年龄患者PCR检出率存在差异，且脓血便可作为判断志贺菌感染程度的指标之一。
Abstract:
Abstract：Objective To compare the effects of bacterial culture and polymerase chain reaction (PCR) detection in the detection of bacterial dysentery.Methods A total of 220 patients with diarrhea diagnosed in our hospital from January 2018 to January 2019 were enrolled in this study. Bacterial culture and polymerase chain reaction (PCR) were used to detect fecal specimens. Compare the detection rate of two methods and the impact of basic situation of patients on the detection rate.Results The PCR detection rate was 50.45% higher than that of bacterial culture 4.54%, and the detection rate of PCR was higher than that of bacterial culture method,the difference was statistically significant (P<0.05). In PCR detection, culture was negative and non-culture. There was a statistically significant difference in the average number of PCR cycles between pus and bloody stools, age≥5 years and culture-positive, pus and bloody stools, there was a statistically significant difference in the mean number of PCR cycles between the ages <5 years old (P<0.05).Conclusion PCR detection and bacterial culture in the detection of bacterial dysentery have a more accurate evaluation, which is conducive to clinical evaluation of bacterial dysentery, and has important clinical significance. At the same time, the PCR detection rates of patients of different ages are different, and pus and blood can be used as one of the indicators to judge the degree of Shigella infection.

参考文献/References:

[1]许丽霞,黄剑芳,刘集鸿.细菌培养与聚合酶链反应（PCR）检测在细菌性痢疾检测中的应用分析[J].检验医学与临床,2014,15(12):1676-1677. [2]王蒋丽,李彩云,曾强,等.荧光PCR法与细菌培养法在食源性致病检测中的应用[J].中国卫生检验杂志,2015,21(10):2498-2499. [3]王晶晶,陈爱英,阎碧茹.211例细菌性痢疾便标本细菌培养及药敏试验结果[J].山西医科大学学报,2015,29(2):125-127. [4]曾桂芬,龙北国,姜容,等.LAM和PCR法检测痢疾志贺菌的特异性和灵敏性比较[J].热带医学杂志,2014,12(5):547-549 [5]王国富,吴利先,薛士鹏.志贺菌感染的多重PCR快速诊断技术的建立[J].中国现代药物应用,2015,6(12):130-135.

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更新日期/Last Update: 2019-09-01