[1]付晓春,沈小莉,李红群,等.雷公藤多苷促进斑马鱼肝脏损伤和氧化应激的实验研究[J].医学信息,2019,32(05):79.[doi:10.3969/j.issn.1006-1959.2019.05.024]
　FU Xiao-chun,SHEN Xiao-li,LI Hong-qun,et al.Experimental Study on the Effects of Tripterygium Glycosides on Liver Damage and Oxidative Stress in Zebrafish[J].Medical Information,2019,32(05):79.[doi:10.3969/j.issn.1006-1959.2019.05.024]
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雷公藤多苷促进斑马鱼肝脏损伤和氧化应激的实验研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
32卷
期数:
2019年05期
页码:
79
栏目:
论著
出版日期:
2019-03-01

文章信息/Info

Title:
Experimental Study on the Effects of Tripterygium Glycosides on Liver Damage and Oxidative Stress in Zebrafish
文章编号:
1006-1959（2019）05-0079-05
作者:
付晓春1; 沈小莉1; 李红群2; 朱家乐2; 蒋 平1
（1.广东食品药品职业学院，广东 广州 510520；2.杭州环特生物科技股份有限公司，浙江 杭州 310051）
Author(s):
FU Xiao-chun1; SHEN Xiao-li1; LI Hong-qun2; ZHU Jia-le2; JIANG Ping1
(1.Guangdong Food and Drug Vocational College,Guangzhou 510520,Guangdong,China;2.Hangzhou Huante Biotechnology Co.,Ltd.,Hangzhou 310051,Zhejiang,China)
关键词:
雷公藤多苷; 斑马鱼; 肝损伤; 氧化应激
Keywords:
Tripterygium glycosides; Zebrafish; Liver injury; Oxidative stress
分类号:
R285.5
DOI:
10.3969/j.issn.1006-1959.2019.05.024
文献标志码:
A
摘要:
目的 研究雷公藤多苷诱导斑马鱼肝脏损伤及氧化应激的作用。方法 随机选取正常3 dpf 野生型AB品系斑马鱼，分为7组：正常对照组、溶剂对照组（0.5% DMSO）、雷公藤多苷300、350、400、450和500 μg/ml浓度处理组，每组30尾斑马鱼。雷公藤多苷处理斑马鱼直至120 hpf（3 dpf~5 dpf），观察记录每个实验组斑马鱼的死亡情况并计算死亡率，用Origin 8.0统计学软件绘制最佳的“浓度-死亡率”效应曲线并分别计算雷公藤多苷对斑马鱼的MNLC和LC10。另随机选取正常3 dpf野生型AB品系斑马鱼，分为5组：正常对照组、对乙酰氨基酚（8 mM）组、雷公藤多苷片42.5、128和383 μg/ml浓度组，每组60尾斑马鱼。药物处理48 h后，每组随机选取30条斑马鱼置于4%多聚甲醛固定，固定后将斑马鱼转移到70%乙醇中，进行脱水、包埋、切片、HE染色和封片，在显微镜下对染色后的斑马鱼切片进行肝脏病理学分析；在雷公藤多苷处理48 h后，每组随机取30尾斑马鱼收集并处理检测样本，检测过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。结果 雷公藤多苷对斑马鱼的MNLC为383 μg/ml，LC10为417 μg/ml。HE染色结果提示：对乙酰氨基酚对斑马鱼有明显肝毒性，随着雷公藤多苷片浓度增加，斑马鱼肝脏组织结构破坏程度增大。对乙酰氨基酚组、雷公藤多苷片42.5、128和383 μg/ml浓度组的斑马鱼体内CAT、SOD活力以及MDA含量，与正常对照组比较，差异无统计学意义（P＞0.05）。结论 雷公藤多苷可通过诱导氧化应激促进斑马鱼的肝损伤。
Abstract:
Objective To study the effects of tripterygium glycosides on liver damage and oxidative stress in zebrafish. Methods Normal 3 dpf wild type AB strain zebrafish were randomly selected and divided into 7 groups: normal control group, solvent control group (0.5% DMSO), tripterygium glycosides 300, 350, 400, 450 and 500 μg/ml concentration treatment group, each group of 30 zebrafish. Tripterygium wilfordii treatment of zebrafish up to 120 hpf (3 dpf~5 dpf), observe the death of zebrafish in each experimental group and calculate the mortality, and use the Origin 8.0 statistical software to draw the best "concentration-mortality" The effect curves were calculated and the MNLC and LC10 of zebrafish were calculated for tripterygium glycosides. The normal 3 dpf wild type AB strain zebrafish were randomly selected and divided into 5 groups: normal control group, acetaminophen (8 mM) group, tripterygium glycosides 42.5, 128 and 383 μg/ml concentration group, each group 60 zebrafish. After 48 h of drug treatment, 30 zebrafish were randomly selected from each group and fixed in 4% paraformaldehyde. After fixation, the zebrafish were transferred to 70% ethanol for dehydration, embedding, sectioning, HE staining and sealing. The liver pathological analysis of the stained zebrafish sections was performed under microscope. After 48 hours of treatment with tripterygium glycosides, 30 zebrafish were randomly selected from each group to collect and process the test samples to detect catalase (CAT) and superoxide. Dismutase (SOD) activity and malondialdehyde (MDA) content. Results Tripterygium glycosides had a MNLC of 383 μg/ml for zebrafish and 417 μg/ml for LC10. The results of HE staining indicated that acetaminophen had obvious hepatotoxicity to zebrafish. With the increase of the concentration of tripterygium glycosides, the damage of liver tissue structure of zebrafish increased. The CAT, SOD activity and MDA content of zebrafish in the acetaminophen group and tripterygium glycosides group 42.5, 128 and 383 μg/ml were not significantly different from those in the normal control group (P>0.05). Conclusion Tripterygium glycosides can promote liver damage in zebrafish by inducing oxidative stress.

参考文献/References:


[1]刘升.雷公藤多苷治疗慢性特发性荨麻疹效果分析[J].中国卫生标准管理,2018,9(18):104-105.
[2]陈菁,王彤.雷公藤多苷片用于慢性肾炎治疗的效果评价[J].中国处方药,2018,16(9):77-78.
[3]张红,薛中柱.雷公藤多苷联合甲氨蝶呤治疗类风湿性关节炎短期疗效研究[J].吉林中医药,2018,38(6):660-663.
[4]王萍.雷公藤多苷联合激素治疗中度活动型系统性红斑狼疮的临床效果分析[J].中国实用医药,2018,13(13):99-100.
[5]杨冬梅,刘俊.雷公藤多苷临床应用及不良反应的研究进展[J].中国医院药学杂志,2018,38(20):1-6.
[6]印成霞.55例雷公藤多甙片/雷公藤多苷片致不良反应文献分析[J].中国药物警戒,2013,10(8):478-482.
[7]赵叶.182例雷公藤多苷片不良反应分析及预防措施[J].山东中医杂志,2012,31(8):572-574.
[8]杨威,黄远铿,雷夏凌,等. 雷公藤多苷对消化道的毒性研究[C]//中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛论文集.2015.
[9]曹艳,运乃茹,邹爱英.雷公藤多苷片致不良反应的Meta分析[J].中国药房,2018,29(1):125-130.
[10]李向阳,张静岩.雷公藤引起纯红细胞再生障碍性贫血1例[J].中国误诊学杂志,2010,10(22):5537.
[11]罗岚,江振洲,张陆勇.雷公藤多苷肝毒性发生机制及减毒相关研究进展[J].药物评价研究,2017,40(10):1504-1509.
[12]王晓东,叶桃,金城,等.雷公藤多苷肝毒性血清药物化学的初步研究[J].中国现代中药,2017,19(3):362-371.
[13]罗岚,俞沁玮,江振洲,等.雷公藤多苷肝毒性机制及物质基础研究[C]//2016年第六届全国药物毒理学年会论文集.2016.
[14]唐旭霞,谌晶晶,李斌,等.雷公藤多甙对斑马鱼听毒性和肾毒性的实验研究[C]//中华中医药学会耳鼻喉科分会第二十三次学术年会、世界中联耳鼻喉口腔科专业委员会第九次学术年会论文集.2017.
[15]任强,岑国栋,高永翔.雷公藤多苷诱导大鼠肾细胞凋亡的NF-кB信号转导通路机制研究[J].成都中医药大学学报,2011,34(2):39-44.
[16]信长慧,张竞超,张付菊.雷公藤多苷片对高脂小鼠血脂的影响[J].中国现代应用药学,2018,35(9):1351-1354.
[17]谢佳宏.雷公藤多苷对PCI患者冠脉支架内再狭窄发生率及远期预后的影响[D].青岛大学,2017.
[18]郭健敏,黄远铿,雷夏凌,等.雷公藤多苷对雄性SD大鼠生殖系统损伤的动态变化及其可能机制[J].中国药理学与毒理学杂志,2018,32(6):469-476.
[19]任献青,郑贵珍,苏杭,等.菟丝子黄酮对雷公藤多苷片致生精细胞周期阻滞、凋亡及相关蛋白表达降低的影响[J].药物评价研究,2018,41(1):55-60.
[20]张婷婷,马晓莉.雷公藤多苷致卵巢早衰1例[J].解放军医学院学报,2017,38(12):1167-1168.
[21]张妍君.系统性红斑狼疮与狼疮性肾炎的药物治疗进展[D].遵义医学院,2018.
[22]马一旻,卜建宏.狼疮性肾炎的治疗进展[J].医学综述,2018,24(7):1334-1338.
[23]余志谋.类风湿性关节炎的临床诊断及药物治疗进展[J].现代诊断与治疗,2018,29(5):704-705.
[24]Wiegand C,Pflugmacher S,Giese M,et al.Uptake, toxicity, and effects on detoxication enzymes of atrazine and trifluoroacetate in embryos of zebrafish[J].Ecotoxicol Environ Saf,2000,45(2):122-131.
[25]Braunbeck T,Gorge G,Storch V,et al.Hepatic steatosis in zebra fish (Brachydanio rerio) induced by long-term exposure to gamma-hexachlorocyclohexane[J].Ecotoxicol Environ Saf,1990,19(3):355-374.
[26]禄保平,胡丹华,杨晓慧.保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠自由基脂质过氧化反应的影响[J].西安交通大学学报(医学版),2008,29(3):333-335.
[27]Wheeler MD,Nakagami M,Bradford BU,et al.Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat[J].J Biol Chem,2001,276(39):36664-36672.
[28]赵小梅,刘歆颖,续畅,等.基于LC-MS代谢组学的雷公藤多苷致肝毒性生物标志物的初步筛查[J].中国中药杂志,2015,40(19):3851-3858.
[29]Yao J,Jiang Z,Duan W,et al.Involvement of mitochondrial pathway in triptolide-induced cytotoxicity in human normal liver L-02 cells[J].Biol Pharm Bull,2008,31(4):592-597.
[30]Mei Z,Li X,Wu Q,et al.The research on the anti-inflammatory activity and hepatotoxicity of triptolide-loaded solid lipid nanoparticle[J].Pharmacol Res,2005,51(4):345-351.
[31]刘史佳,刘子修,周玲,等.雷公藤活性成分对大鼠体内P450酶活性的影响[J].中国药理学通报,2011,27(2):276-280.
[32]夏军,夏宗玲.雷公藤多苷对大鼠与人肝微粒体CYP3A酶活性的体外抑制作用[J].中国药房,2015,26(4):473-476.
[33]吴利平.氧化应激与心血管疾病关系的研究进展[J].中国处方药,2017,15(7):9-10.
[34]李健,司丽芳,李梦云,等.氧化应激损伤雄性生殖功能的研究进展[C]//中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集.2016.

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更新日期/Last Update: 2019-03-01