[1]张 震,魏屹东,汪伟基,等.酒精联合激素加速兔早期股骨头坏死的模型建立及病理学研究[J].医学信息,2020,33(08):67-69.[doi:10.3969/j.issn.1006-1959.2020.08.022]
　ZHANG Zhen,WEI Yi-dong,WANG Wei-ji,et al.Alcohol Combined with Hormones Accelerates Early Femoral Head Necrosis Model Establishment and Pathological Study in Rabbits[J].Medical Information,2020,33(08):67-69.[doi:10.3969/j.issn.1006-1959.2020.08.022]
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酒精联合激素加速兔早期股骨头坏死的模型建立及病理学研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年08期
页码:
67-69
栏目:
论著
出版日期:
2020-04-15

文章信息/Info

Title:
Alcohol Combined with Hormones Accelerates Early Femoral Head Necrosis Model Establishment and Pathological Study in Rabbits
文章编号:
1006-1959（2020）08-0067-03
作者:
张 震; 魏屹东; 汪伟基; 等
（新疆军区总医院创伤骨科，新疆 乌鲁木齐 830000）
Author(s):
ZHANG Zhen; WEI Yi-dong; WANG Wei-ji; et al
(Department of Traumatology and Orthopedics,Xinjiang Military Region General Hospital,Urumqi 830000,Xinjiang,China)
关键词:
激素性股骨头坏死; 酒精性股骨头坏死; 病理学检查; 动物模型; 兔
Keywords:
Hormonal femoral head necrosis; Alcoholic femoral head necrosis; Pathological examination; Animal model; Rabbit
分类号:
R681.8
DOI:
10.3969/j.issn.1006-1959.2020.08.022
文献标志码:
A
摘要:
目的 检测长期较大剂量摄入酒精联合应用大剂量糖皮质激素能否加速早期股骨头坏死病程的进展，并探讨该方法的有效性。方法 取6月龄新西兰大白兔30只，体重2~3 kg，随机分为三组，空白对照组10只，实验1组（单纯使用糖皮质激素）10只，实验2组（联合使用糖皮质激素及酒精）10只。实验1组于双侧臀肌交替肌肉注射地塞米松磷酸钠注射液10 ml/kg，每3 d 1次，共10次；实验2组在实验1组基础上给予饮用酒灌胃8 ml/kg，每3 d 1次，共10次；空白对照组于双侧臀肌交替注射等量生理盐水，正常饮食、饮水，造模期间观察实验动物一般情况，30 d后观察股骨头病理学检查。结果 空白对照组动物未出现脱毛、食量减小及运动改变；实验1组出现轻微脱毛，无跛行，无食量改变；实验2组饮食、饮水明显减少，有脱毛及轻微跛行。三组股骨头进行冠状面剖开肉眼观察未见明显差别。病理学观察示，空白对照组骨小梁粗大，骨陷窝内骨细胞形态正常，脂肪细胞较少；实验1组骨小梁变薄，空骨陷窝增多，骨细胞核固缩；实验2组骨小梁变薄，核固缩的骨细胞及空骨陷窝明显增多。实验1组，实验2组，空白对照组空骨陷窝率分别为（8.85±0.99）%、（33.20±1.51）%、（51.30±1.87）%，差异有统计学意义（P＜0.05）。结论 短期、大剂量给予地塞米松成功构建兔早期激素性股骨头坏死模型，同时在给予激素的过程中加用较大剂量酒精能够加速股骨头坏死的病程，可通过病理学检查对模型进行有效性评价。
Abstract:
Objective To investigate whether long-term large-dose alcohol intake combined with high-dose glucocorticoids can accelerate the progression of early femoral head necrosis and explore the effectiveness of this method.Methods 30 New Zealand white rabbits of 6 months old, weighing 2 to 3 kg, were randomly divided into three groups,there were 10 blank control groups, 10 in the experimental group 1 (using glucocorticoids alone), and 10 in the experimental group 2(using glucocorticoids and alcohol together). The experimental group 1 was alternately intramuscularly injected with 10 ml/kg of dexamethasone sodium phosphate injection into both gluteal muscles, once every 3 d for a total of 10 times; the experimental group 2 was given 8 ml/kg of drinking wine on the basis of the experimental group 1, Once every 3 d, a total of 10 times; the control group was alternately injected with bilateral saline muscles with the same amount of normal saline, normal diet and drinking water, and observed the general conditions of experimental animals during the modeling period, and observed the femoral head pathology after 30 d.Results The animals in the control group showed no hair loss, reduced food intake and exercise changes; the experimental group 1 had slight hair loss, no claudication, and no changes in food intake; the experimental group 2 had significantly reduced diet and drinking water, and had hair loss and slight lameness. The three groups of femoral heads showed no obvious difference by coronal plane dissection.Pathological observation showed that the bone trabecula in the control group was thick, the bone cell morphology was normal in the bone lacuna, and there were fewer fat cells; the bone trabecula in the experimental group 1 was thinner, the empty bone lacuna increased, and the bone cell nucleus was contracted,in the experimental group 2, the trabecular bones became thinner, and the bone cells and empty bone lacunae increased significantly. The rates of empty bone lacunae in the experimental group 1 and experimental group 2 were (8.85±0.99)%, (33.20±1.51)%, and (51.30±1.87)%, respectively,the difference was statistically significant (P<0.05).Conclusion Short-term and high-dose administration of dexamethasone successfully constructed a rabbit model of early hormonal femoral head necrosis. At the same time, the addition of larger doses of alcohol during the administration of hormones can accelerate the course of femoral head necrosis. Pathological examination can be effective for the model Sexual evaluation.

参考文献/References:

[1]张立岩,孙新,田丹,等.兔早期激素性股骨头缺血性坏死模型建立及其MRI与病理特征研究[J].中国修复重建外科杂志,2015,29(10):1240-1243.[2]董维,郭杨,马勇,等.甲强龙联合脂多糖诱导兔股骨头坏死模型的建立[J].中国骨质疏松杂志,2016,22(4):402-405.[3]曹良权,杜斌,孙光权,等.不同剂量糖皮质激素联合马血清构建激素诱导型股骨头缺血性坏死兔模型[J].中国组织工程研究,2017,21(8):1229-1235.[4]李超,尚希福,李旭,等.提高激素性股骨头坏死兔模型动物存活率的实验研究[J].安徽医科大学学报,2014,49(12):1726-1729.[5]陈雁飞,吴吉荣.糖皮质激素致股骨头坏死的病理生理机制研究进展[J].解放军药学学报,2017,33(6):566-569,587.[6]陈亦轩,朱道宇,殷俊辉,等.酒精性股骨头坏死研究进展[J].国际骨科学杂志,2018,39(1):28-32.[7]席立成,李宏宇.酒精对股骨头局部微环境影响的机制研究进展[J].中国组织工程研究,2017,21(7):1137-1142.[8]Wang YH,Zhang CL,Tian SQ,et al.Establishment of steroid-induced necrosis of femoral head [J].Journal of Clinical Rehabilitative Tissue Engineering Research,2013,17(50):8729-8734.[9]赵道洲,李兴国,代长泉,等.兔激素性股骨头坏死造模中死亡因素与对策[J].影像研究与医学应用,2017,1(18):149-151.[10]Powell C,Chang C,Gershwin ME.Current concepts on the pathogenesis and natural history of steroid-induced osteonecrosis[J].Clin Rev Allergy Immunol,2011,41(1):102-113.[11]曲春涛.构建双足类大型鸟类鸸鹋股骨头坏死模型[J].中国组织工程研究,2016,20(5):611-615.

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更新日期/Last Update: 2020-04-15