[1]江 燕,白 雪,赵立志,等.一种改良兔心房纤颤模型建立方法的研究[J].医学信息,2020,33(18):51-53，65.[doi:10.3969/j.issn.1006-1959.2020.18.016]
　JIANG Yan,BAI Xue,ZHAO Li-zhi,et al.Study on an Improved Method for Establishing Rabbit Atrial Fibrillation Model[J].Medical Information,2020,33(18):51-53，65.[doi:10.3969/j.issn.1006-1959.2020.18.016]
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一种改良兔心房纤颤模型建立方法的研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年18期
页码:
51-53，65
栏目:
论著
出版日期:
2020-09-15

文章信息/Info

Title:
Study on an Improved Method for Establishing Rabbit Atrial Fibrillation Model
文章编号:
1006-1959（2020）18-0051-04
作者:
江 燕; 白 雪; 赵立志; 等
（西南医科大学附属中医院心内科，四川 泸州 646000）
Author(s):
JIANG Yan; BAI Xue; ZHAO Li-zhi; et al
(Department of Cardiology,the Affiliated Hospital of Traditional Chinese Medicine,Southwest Medical University,Luzhou 646000,Sichuan,China)
关键词:
心房纤颤; 兔; 动物模型; 改良
Keywords:
Atrial fibrillation; Rabbit; Animal model; Improvement
分类号:
R541.75
DOI:
10.3969/j.issn.1006-1959.2020.18.016
文献标志码:
A
摘要:
目的 寻找一种操作简便、可重复性高的房颤建模方法，为研究心房纤颤的发生机制以及治疗方案奠定基础。方法 将新西兰兔30只随机分为正常对照组、常规模型组、改良组，每组各10只，正常对照组仅暴露心房和分离肺静脉，J形双极起搏电极置于左心房，仅置入电极而不起搏；常规模型组暴露心房分离肺静脉后将起搏电极缝在左心房，高频起搏左心房起搏12 h；改良组暴露心房，分离肺静脉，然后在肺静脉主干任意处使用自制双电极电刺激，进行程序期前刺激12 h，比较三组存活情况、房颤诱发率、房颤发作的心室率及房颤持续时间，测量完毕后分离左心耳进行HE染色，观察三组左心耳纤维化程度。结果 三组死亡率均为10.00%，差异无统计学意义（P＞0.05）；正常对照组未诱发出心律失常，常规模型组房颤有诱发率为50.00%，改良组诱发率为80.00%，差异有统计学意义（P＜0.05）；常规模型组房颤发作时平均心室率为（121.00±15.35）次/min，低于改良组的（154.00±18.49）次/min，差异有统计学意义（P＜0.05）；常规模型组房颤持续时间为（425.40±49.51）s，低于改良组的（817.60±39.16）s，差异有统计学意义（P＜0.05）；改良组每倍镜下心肌纤维化及细胞空泡程度高于常规模型组及对照组。结论 兔心房纤颤时左心耳的纤维化程度较正常窦性心律时增加，房颤发生时心耳也出现结构重构，本次改良方法可成功建立心房纤颤模型，且成功率高、方法简单、重复性好，值得推广。
Abstract:
Objective To find a simple and reproducible atrial fibrillation modeling method to lay the foundation for the study of the mechanism of atrial fibrillation and treatment options. Methods 30 New Zealand rabbits were randomly divided into normal control group, conventional model group, and modified group, each with 10 rabbits. The normal control group only exposed the atrium and separated the pulmonary vein. The J-shaped bipolar pacing electrode was placed in the left atrium and only placed the electrode is inserted without pacing; the conventional model group was exposed to the atrium and the pulmonary vein is separated, the pacing electrode was sewn to the left atrium, and the left atrium was paced with high frequency pacing for 12 h; the modified group was exposed to the atrium, separates the pulmonary vein, and then use it anywhere in the pulmonary vein self-made dual-electrode electrical stimulation, performed pre-procedural stimulation for 12 h, compared the survival, atrial fibrillation induction rate, ventricular rate of atrial fibrillation and the duration of atrial fibrillation in the three groups. After the measurement, the left atrial appendage was separated for HE staining, and the three groups were observed The degree of left atrial appendage fibrosis.Results The mortality rate of the three groups was 10.00%,the difference was not statistically significant (P>0.05); the normal control group did not induce arrhythmia, the induction rate of atrial fibrillation in the conventional model group was 50.00%, and the induction rate of the modified group was 80.00%,the difference was statistically significant (P<0.05); the average ventricular rate during the onset of atrial fibrillation in the conventional model group was (121.00±15.35) beats/min, which was lower than that of the modified group (154.00±18.49) beats/min,the difference was statistically significant (P＜0.05); The duration of atrial fibrillation in the conventional model group was (425.40±49.51) s, which was lower than (817.60±39.16) s in the modified group,the difference was statistically significant (P＜0.05); The degree of muscle fibrosis and cell vacuolation was higher than that of the conventional model group and the control group.Conclusion The degree of fibrosis of the left atrial appendage in rabbits with atrial fibrillation was higher than that of normal sinus rhythm, and the structure of the atrial appendage was also remodeled when atrial fibrillation occurs. This modified method could successfully establish atrial fibrillation model with high success rate and simple method,good repeatability, worthy of promotion.

参考文献/References:

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更新日期/Last Update: 1900-01-01