[1]陈 琛,许安平.EMSCs复合CPC支架体外生物相容性的研究[J].医学信息,2020,33(23):68-72.[doi:10.3969/j.issn.1006-1959.2020.23.020]
　CHEN Chen,XU An-ping.Study on in Vitro Biocompatibility of EMSCs Composite CPC Scaffold[J].Medical Information,2020,33(23):68-72.[doi:10.3969/j.issn.1006-1959.2020.23.020]
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EMSCs复合CPC支架体外生物相容性的研究()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
33卷
期数:
2020年23期
页码:
68-72
栏目:
论著
出版日期:
2020-12-01

文章信息/Info

Title:
Study on in Vitro Biocompatibility of EMSCs Composite CPC Scaffold
文章编号:
1006-1959（2020）23-0068-05
作者:
陈 琛; 许安平
（安徽省铜陵市人民医院骨科中心，安徽 铜陵 244000）
Author(s):
CHEN Chen; XU An-ping
(Orthopedic Center,Tongling People’s Hospital,Tongling 244000,Anhui,China)
关键词:
EMSCs; CPC; 生物相容性
Keywords:
EMSCs; CPC; Biocompatibility
分类号:
R318.08
DOI:
10.3969/j.issn.1006-1959.2020.23.020
文献标志码:
A
摘要:
目的 观察磷酸钙骨水泥（CPC）对鼻粘膜间充质干细胞（EMSCs）体外诱导成骨的影响。方法 取出SD大鼠的全层鼻粘膜行体外培养并扩增EMSCs，选取第三代生长状况良好的EMSCs与CPC混合培养，行成骨诱导14天。将EMSCs/CPC设定为CPC材料组，单纯EMSCs培养为对照组。ALP检测两组EMSCs碱性磷酸酶活性，茜素红染色法测定钙结节面积大小，免疫印迹法检测成骨诱导相关蛋白表达水平；鬼笔环肽染色法动态观察EMSCs在CPC表面的细胞形态；MTT法检测CPC对EMSCs增殖的影响，并进行统计学分析。结果 ①CPC材料组较对照组中碱性磷酸酶活性在成骨诱导14 d后较高，染色程度深；茜素红染色中CPC材料组的钙结节更大；鬼笔环肽染色液染色第3天EMSCs形态呈圆形，细胞形态未完全伸展，第7天EMSCs呈长梭型，第14天后EMSCs成堆生长；免疫印迹法检测CPC材料组表达collagen I和osteocalcin高于对照组（P＜0.05）；②细胞接种后，CPC材料组和对照组随在前5 d内保持正常的增殖速度，两者之间无明显差异，第7天开始可见明显的促进作用，两组比较，差异有统计学意义（P＜0.05）。结论 CPC是一种优良的组织工程支架材料，能够促进EMSCs体外增殖以及诱导成骨，EMSCs与CPC之间具有良好的生物相容性。
Abstract:
Objective To observe the effect of calcium phosphate cement (CPC) on the induction of osteogenesis of nasal mucosal mesenchymal stem cells (EMSCs) in vitro.Methods The full-thickness nasal mucosa of SD rats was taken out and cultured in vitro and expanded EMSCs. The third-generation EMSCs with good growth condition were mixed and cultured with CPC, and osteogenic induction was performed for 14 d. Set EMSCs/CPC as the CPC material group, and cultivate pure EMSCs as the control group. ALP was used to detect the alkaline phosphatase activity of the two groups of EMSCs, the alizarin red staining method was used to determine the size of calcium nodules, the Western blotting method was used to detect the expression level of osteoinduction-related proteins; the phalloidin staining method was used to dynamically observe the cell morphology of EMSCs on the CPC surface; MTT method was used to detect the impact of CPC on the proliferation of EMSCs and perform statistical analysis.Results ①The alkaline phosphatase activity in the CPC material group was higher than that in the control group after 14 d of osteogenic induction, and the staining degree was deeper;In Alizarin Red staining, the calcium nodules in the CPC material group were larger; on the third day of staining with phalloidin staining, the EMSCs were round in shape and the cell morphology was not fully extended. On the 7th day, the EMSCs were in a long spindle shape, and after the 14th day,growth in piles; the expression of collagen I and osteocalcin in the CPC material group detected by western blotting was higher than that of the control group (P＜0.05); ②After cell inoculation, the CPC material group and control group maintained normal proliferation rates within the first 5 d. There was no significant difference between the patients, and the obvious promotion effect was seen on the 7th day,the difference between the two groups was statistically significant(P<0.05).Conclusion CPC is an excellent scaffold material for tissue engineering, which can promote the proliferation of EMSCs and induce bone formation in vitro. There is good biocompatibility between EMSCs and CPC.

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更新日期/Last Update: 1900-01-01