[1]覃媛媛,沈嘉华.HPLC法测定复方大红袍止血胶囊中没食子酸的含量[J].医学信息,2021,34(16):98-101.[doi:10.3969/j.issn.1006-1959.2021.16.026]
　QIN Yuan-yuan,SHEN Jia-hua.Determination of Gallic Acid in Compound Dahongpao Zhixue Capsules by HPLC[J].Medical Information,2021,34(16):98-101.[doi:10.3969/j.issn.1006-1959.2021.16.026]
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HPLC法测定复方大红袍止血胶囊中没食子酸的含量()
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医学信息[ISSN:1006-1959/CN:61-1278/R]

卷:
34卷
期数:
2021年16期
页码:
98-101
栏目:
论著
出版日期:
2021-08-15

文章信息/Info

Title:
Determination of Gallic Acid in Compound Dahongpao Zhixue Capsules by HPLC
文章编号:
1006-1959（2021）16-0098-04
作者:
覃媛媛; 沈嘉华
（楚雄州食品药品检验所，云南 楚雄 675000）
Author(s):
QIN Yuan-yuan; SHEN Jia-hua
(Chuxiong Prefecture Food and Drug Inspection Institute,Chuxiong 675000,Yunnan,China)
关键词:
复方大红袍止血胶囊; 质量标准; 没食子酸; 高效液相色谱法
Keywords:
Compound dahongpao zhixue capsules; Quality standard; Gallic acid; High performance liquid chromatography
分类号:
R284.1
DOI:
10.3969/j.issn.1006-1959.2021.16.026
文献标志码:
A
摘要:
目的 建立HPLC方法测定复方大红袍止血胶囊中没食子酸的含量。方法 采用Agilent ZORBAX SB-C18色谱柱（5 μm，4.6 mm×250 mm）；流动相为甲醇-0.05%磷酸溶液（5∶95）；流速1.0 ml/min；检测波长272 nm；进样量10 μl；柱温30 ℃。结果 本方法进样量在1.832~27.485 μg/ml范围内与峰面积呈良好的线性关系（r2=1.0000）；平均加样回收率（n=6）为96.58%（RSD=1.42%）；采用外标法计算10批样品中的没食子酸含量测定结果为0.28~0.35 mg/粒。结论 HPLC法简便可行，结果准确可靠，重复性好，可用于复方大红袍止血胶囊的质量控制。
Abstract:
Objective To establish an HPLC method for the determination of gallic acid in compound dahongpao zhixue capsules.Methods Agilent ZORBAX SB-C18 column (5 μm, 4.6 mm×250 mm) was used;The mobile phase is methanol-0.05% phosphoric acid solution (5:95); the flow rate was 1.0 ml/min; the detection wavelength was 272 nm; the injection volume was 10 μl; the column temperature was 30 ℃.Results The sample injection volume of this method showed a good linear relationship with the peak area in the range of 1.832~27.485 μg/ml (r2=1.0000); the average sample recovery (n=6) was 96.58% (RSD=1.42%);The external standard method was used to calculate the gallic acid content in 10 batches of samples and the results were 0.28~0.35 mg/grain.Conclusion HPLC method is simple, feasible, accurate, reliable, and reproducible. It can be used for the quality control of compounddahongpao zhixue capsules.

参考文献/References:

[1]国家药典委员会.中华人民共和国药典:2015年版一部[M].北京:中国医药科技出版社,2015. [2]黄文平,黄陆强,宋永贵,等.不同产地柿蒂药材中没食子酸含量的比较[J].江西中医药大学学报,2014,26(3):58-59,62. [3]Smrke T,Persic M,Veberic R,et al.Influence of reflective foil on persimmon (Diospyros kaki Thunb.) fruit peel colour and selected bioactive compounds[J].Sci Rep,2019,9(1):19069. [4]吴茱萸.没食子酸对肺癌细胞增殖凋亡影响及可能机制研究[D].郑州:郑州大学,2017. [5]田衍,舒若,罗华友.没食子酸对PI3K/AKT基因表达的影响及其对胃癌细胞的抗转移作用[J].基因组学与应用生物学,2020,39(2):884-889.

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更新日期/Last Update: 1900-01-01